TY - JOUR
T1 - Microstructural changes in acid milk gels due to temperature-controlled high-intensity ultrasound treatment
T2 - Quantification by analysis of super-resolution microscopy images
AU - James Glover, Zachary
AU - Gregersen, Sandra Beyer
AU - Wiking, Lars
AU - Hammershøj, Mariannne
AU - Simonsen, Adam Cohen
N1 - Funding Information:
This work was funded by the Danish Dairy Research Fund, Arla Foods amba, Arla Foods Ingredients P/S, Aarhus University and University of Southern Denmark.
Publisher Copyright:
© 2021 Society of Dairy Technology
PY - 2022/5
Y1 - 2022/5
N2 - High-intensity ultrasound (HIU) can be applied to food materials to create novel structures and textures, and as a method of processing. HIU treatment of milk modifies the gelation behaviour, but the microstructural impact is poorly understood. Milk samples were treated with HIU at three temperatures: 27°C, 50°C, and 70°C. Acid milk gels were formed from the milk and imaged using super-resolution stimulated emission depletion and confocal microscopy. Quantitative image analysis was applied and demonstrated that HIU treatment leads to measurable changes in both protein network morphology and degree of association of the fat droplets with the protein network.
AB - High-intensity ultrasound (HIU) can be applied to food materials to create novel structures and textures, and as a method of processing. HIU treatment of milk modifies the gelation behaviour, but the microstructural impact is poorly understood. Milk samples were treated with HIU at three temperatures: 27°C, 50°C, and 70°C. Acid milk gels were formed from the milk and imaged using super-resolution stimulated emission depletion and confocal microscopy. Quantitative image analysis was applied and demonstrated that HIU treatment leads to measurable changes in both protein network morphology and degree of association of the fat droplets with the protein network.
KW - High-intensity ultrasound
KW - milk acid gel microstructure
KW - Quantitative image analysis
KW - Stimulated emission depletion microscopy
KW - Super-resolution microscopy
U2 - 10.1111/1471-0307.12838
DO - 10.1111/1471-0307.12838
M3 - Journal article
AN - SCOPUS:85120825139
VL - 75
SP - 321
EP - 328
JO - International Journal of Dairy Technology
JF - International Journal of Dairy Technology
SN - 1364-727X
IS - 2
ER -