Metabolomics and traditional indicators unveil stress of a seagrass (Cymodocea nodosa) meadow at intermediate distance from a fish farm

Willemien de Kock, Harald Hasler-Sheetal, Marianne Holmer, Manolis Tsapakis, Eugenia T. Apostolaki

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Seasonal variation of structural, physiological and growth indicators and the metabolome of the seagrass Cymodocea nodosa, as well as biogeochemical conditions of underlying sediment were studied in two meadows growing at increasing distance downstream from a fish farm in the Aegean Sea in order to assess seagrass performance under stress. Horizontal rhizome production decreased significantly with proximity to the fish farm (0.67 and 1.57 g DW m−2 d−1 close and far from the fish-farm, respectively). This coincided with observed effects on ecophyiological indicators, such as rhizome nitrogen, leaf carbon and leaf δ13C, which were elevated with proximity to the fish-farm. Seasonality was shown by some indicators being elevated in either in the warm (C of all tissues and leaf δ34S) or the cold period (N of all tissues). Growth promoting metabolites (sucrose, fructose, myo-inositol, heptacosane, tetracosane, stigmasterol, catechin and alpha-tocopherol) were lower close to the zone, whereas metabolites involved with stress-response (alanine, serine, proline, putrescine, ornithine, 3,4-dihydroxybenzoic acid and cinnamic acid) were higher. We found that growth-promoting metabolites were positively correlated with horizontal rhizome production, whereas the metabolites related to stress were negatively correlated. Metabolomic fingerprinting of seagrass provides opportunities for early detection of environmental degradation in marine ecological studies.
OriginalsprogEngelsk
TidsskriftEcological Indicators
Vol/bind109
ISSN1470-160X
DOI
StatusUdgivet - 2020

Fingeraftryk

fish farms
metabolomics
seagrass
meadow
meadows
metabolite
rhizome
farm
rhizomes
metabolites
fish
leaves
metabolome
Aegean Sea
stigmasterol
ornithine
cinnamic acid
environmental degradation
acid
putrescine

Citer dette

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title = "Metabolomics and traditional indicators unveil stress of a seagrass (Cymodocea nodosa) meadow at intermediate distance from a fish farm",
abstract = "Seasonal variation of structural, physiological and growth indicators and the metabolome of the seagrass Cymodocea nodosa, as well as biogeochemical conditions of underlying sediment were studied in two meadows growing at increasing distance downstream from a fish farm in the Aegean Sea in order to assess seagrass performance under stress. Horizontal rhizome production decreased significantly with proximity to the fish farm (0.67 and 1.57 g DW m−2 d−1 close and far from the fish-farm, respectively). This coincided with observed effects on ecophyiological indicators, such as rhizome nitrogen, leaf carbon and leaf δ13C, which were elevated with proximity to the fish-farm. Seasonality was shown by some indicators being elevated in either in the warm (C of all tissues and leaf δ34S) or the cold period (N of all tissues). Growth promoting metabolites (sucrose, fructose, myo-inositol, heptacosane, tetracosane, stigmasterol, catechin and alpha-tocopherol) were lower close to the zone, whereas metabolites involved with stress-response (alanine, serine, proline, putrescine, ornithine, 3,4-dihydroxybenzoic acid and cinnamic acid) were higher. We found that growth-promoting metabolites were positively correlated with horizontal rhizome production, whereas the metabolites related to stress were negatively correlated. Metabolomic fingerprinting of seagrass provides opportunities for early detection of environmental degradation in marine ecological studies.",
keywords = "Mediterranean, Early warning indicators, Coastal management, Eutrophication, Omics, Seagrass",
author = "{de Kock}, Willemien and Harald Hasler-Sheetal and Marianne Holmer and Manolis Tsapakis and Apostolaki, {Eugenia T.}",
year = "2020",
doi = "10.1016/j.ecolind.2019.105765",
language = "English",
volume = "109",
journal = "Ecological Indicators",
issn = "1470-160X",
publisher = "Elsevier",

}

Metabolomics and traditional indicators unveil stress of a seagrass (Cymodocea nodosa) meadow at intermediate distance from a fish farm. / de Kock, Willemien; Hasler-Sheetal, Harald; Holmer, Marianne; Tsapakis, Manolis; Apostolaki, Eugenia T.

I: Ecological Indicators, Bind 109, 2020.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Metabolomics and traditional indicators unveil stress of a seagrass (Cymodocea nodosa) meadow at intermediate distance from a fish farm

AU - de Kock, Willemien

AU - Hasler-Sheetal, Harald

AU - Holmer, Marianne

AU - Tsapakis, Manolis

AU - Apostolaki, Eugenia T.

PY - 2020

Y1 - 2020

N2 - Seasonal variation of structural, physiological and growth indicators and the metabolome of the seagrass Cymodocea nodosa, as well as biogeochemical conditions of underlying sediment were studied in two meadows growing at increasing distance downstream from a fish farm in the Aegean Sea in order to assess seagrass performance under stress. Horizontal rhizome production decreased significantly with proximity to the fish farm (0.67 and 1.57 g DW m−2 d−1 close and far from the fish-farm, respectively). This coincided with observed effects on ecophyiological indicators, such as rhizome nitrogen, leaf carbon and leaf δ13C, which were elevated with proximity to the fish-farm. Seasonality was shown by some indicators being elevated in either in the warm (C of all tissues and leaf δ34S) or the cold period (N of all tissues). Growth promoting metabolites (sucrose, fructose, myo-inositol, heptacosane, tetracosane, stigmasterol, catechin and alpha-tocopherol) were lower close to the zone, whereas metabolites involved with stress-response (alanine, serine, proline, putrescine, ornithine, 3,4-dihydroxybenzoic acid and cinnamic acid) were higher. We found that growth-promoting metabolites were positively correlated with horizontal rhizome production, whereas the metabolites related to stress were negatively correlated. Metabolomic fingerprinting of seagrass provides opportunities for early detection of environmental degradation in marine ecological studies.

AB - Seasonal variation of structural, physiological and growth indicators and the metabolome of the seagrass Cymodocea nodosa, as well as biogeochemical conditions of underlying sediment were studied in two meadows growing at increasing distance downstream from a fish farm in the Aegean Sea in order to assess seagrass performance under stress. Horizontal rhizome production decreased significantly with proximity to the fish farm (0.67 and 1.57 g DW m−2 d−1 close and far from the fish-farm, respectively). This coincided with observed effects on ecophyiological indicators, such as rhizome nitrogen, leaf carbon and leaf δ13C, which were elevated with proximity to the fish-farm. Seasonality was shown by some indicators being elevated in either in the warm (C of all tissues and leaf δ34S) or the cold period (N of all tissues). Growth promoting metabolites (sucrose, fructose, myo-inositol, heptacosane, tetracosane, stigmasterol, catechin and alpha-tocopherol) were lower close to the zone, whereas metabolites involved with stress-response (alanine, serine, proline, putrescine, ornithine, 3,4-dihydroxybenzoic acid and cinnamic acid) were higher. We found that growth-promoting metabolites were positively correlated with horizontal rhizome production, whereas the metabolites related to stress were negatively correlated. Metabolomic fingerprinting of seagrass provides opportunities for early detection of environmental degradation in marine ecological studies.

KW - Mediterranean

KW - Early warning indicators

KW - Coastal management

KW - Eutrophication

KW - Omics

KW - Seagrass

U2 - 10.1016/j.ecolind.2019.105765

DO - 10.1016/j.ecolind.2019.105765

M3 - Journal article

VL - 109

JO - Ecological Indicators

JF - Ecological Indicators

SN - 1470-160X

ER -