Metabolic programming determines the lineage-differentiation fate of murine bone marrow stromal progenitor cells

Michaela Tencerova*, Elizabeth Rendina-Ruedy, Ditte Neess, Nils Færgeman, Florence Figeac, Dalia Ali, Morten Danielsen, Anders Haakonsson, Clifford J. Rosen, Moustapha Kassem

*Kontaktforfatter for dette arbejde

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

43 Downloads (Pure)

Abstrakt

Enhanced bone marrow adipogenesis and impaired osteoblastogenesis have been observed in obesity, suggesting that the metabolic microenvironment regulates bone marrow adipocyte and osteoblast progenitor differentiation fate. To determine the molecular mechanisms, we studied two immortalized murine cell lines of adipocyte or osteoblast progenitors (BMSCsadipo and BMSCsosteo, respectively) under basal and adipogenic culture conditions. At baseline, BMSCsadipo, and BMSCsosteo exhibit a distinct metabolic program evidenced by the presence of specific global gene expression, cellular bioenergetics, and metabolomic signatures that are dependent on insulin signaling and glycolysis in BMSCsosteo versus oxidative phosphorylation in BMSCsadipo. To test the flexibility of the metabolic program, we treated BMSCsadipo with parathyroid hormone, S961 (an inhibitor of insulin signaling) and oligomycin (an inhibitor of oxidative phosphorylation). The treatment induced significant changes in cellular bioenergetics that were associated with decreased adipocytic differentiation. Similarly, 12 weeks of a high-fat diet in mice led to the expansion of adipocyte progenitors, enhanced adipocyte differentiation and insulin signaling in cultured BMSCs. Our data demonstrate that BMSC progenitors possess a distinct metabolic program and are poised to respond to exogenous metabolic cues that regulate their differentiation fate.

OriginalsprogEngelsk
Artikelnummer35
TidsskriftBone Research
Vol/bind7
Antal sider14
ISSN2095-4700
DOI
StatusUdgivet - 1. dec. 2019

Fingeraftryk Dyk ned i forskningsemnerne om 'Metabolic programming determines the lineage-differentiation fate of murine bone marrow stromal progenitor cells'. Sammen danner de et unikt fingeraftryk.

Citationsformater