Resumé
Two dimensional phase separation in lipid membranes and cell membranes is of interest to biology because of the idea of membrane rafts — compositionally heterogeneous liquid crystal domains with cellular functions. Few quantitative tools exist for characterizing and differentiating coexisting phases on a molecular scale. Lipid acyl chain order can be measured directly using deuterium nuclear magnetic resonance spectroscopy (2H NMR), or inferred using fluorescence microscopy along with the environment-sensitive probe Laurdan. We found a linear relationship between the 2H NMR order parameter and Laurdan generalized polarization. This observed correlation supports the idea that lipid chain order is tightly associated with the amount and dynamics of water molecules at the glycerol backbone level of the membrane.
| Originalsprog | Engelsk |
|---|---|
| Artikelnummer | 183053 |
| Tidsskrift | Biochimica et Biophysica Acta - Biomembranes |
| Vol/bind | 1861 |
| Udgave nummer | 12 |
| Antal sider | 8 |
| ISSN | 0005-2736 |
| DOI | |
| Status | Udgivet - 1. dec. 2019 |
Fingeraftryk
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Measuring molecular order for lipid membrane phase studies : Linear relationship between Laurdan generalized polarization and deuterium NMR order parameter. / Leung, Sherry See Wai; Brewer, Jonathan; Bagatolli, Luis A.; Thewalt, Jenifer L.
I: Biochimica et Biophysica Acta - Biomembranes, Bind 1861, Nr. 12, 183053, 01.12.2019.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review
TY - JOUR
T1 - Measuring molecular order for lipid membrane phase studies
T2 - Linear relationship between Laurdan generalized polarization and deuterium NMR order parameter
AU - Leung, Sherry See Wai
AU - Brewer, Jonathan
AU - Bagatolli, Luis A.
AU - Thewalt, Jenifer L.
PY - 2019/12/1
Y1 - 2019/12/1
N2 - Two dimensional phase separation in lipid membranes and cell membranes is of interest to biology because of the idea of membrane rafts — compositionally heterogeneous liquid crystal domains with cellular functions. Few quantitative tools exist for characterizing and differentiating coexisting phases on a molecular scale. Lipid acyl chain order can be measured directly using deuterium nuclear magnetic resonance spectroscopy (2H NMR), or inferred using fluorescence microscopy along with the environment-sensitive probe Laurdan. We found a linear relationship between the 2H NMR order parameter and Laurdan generalized polarization. This observed correlation supports the idea that lipid chain order is tightly associated with the amount and dynamics of water molecules at the glycerol backbone level of the membrane.
AB - Two dimensional phase separation in lipid membranes and cell membranes is of interest to biology because of the idea of membrane rafts — compositionally heterogeneous liquid crystal domains with cellular functions. Few quantitative tools exist for characterizing and differentiating coexisting phases on a molecular scale. Lipid acyl chain order can be measured directly using deuterium nuclear magnetic resonance spectroscopy (2H NMR), or inferred using fluorescence microscopy along with the environment-sensitive probe Laurdan. We found a linear relationship between the 2H NMR order parameter and Laurdan generalized polarization. This observed correlation supports the idea that lipid chain order is tightly associated with the amount and dynamics of water molecules at the glycerol backbone level of the membrane.
KW - Deuterium nuclear magnetic resonance spectroscopy
KW - Fluorescence microscopy
KW - Laurdan
KW - Lipid model membrane
KW - Liquid disordered-liquid ordered coexistence
KW - Membrane order
U2 - 10.1016/j.bbamem.2019.183053
DO - 10.1016/j.bbamem.2019.183053
M3 - Journal article
VL - 1861
JO - B B A - Biomembranes
JF - B B A - Biomembranes
SN - 0005-2736
IS - 12
M1 - 183053
ER -