Measuring molecular order for lipid membrane phase studies

Linear relationship between Laurdan generalized polarization and deuterium NMR order parameter

Sherry See Wai Leung, Jonathan Brewer, Luis A. Bagatolli, Jenifer L. Thewalt*

*Kontaktforfatter for dette arbejde

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Two dimensional phase separation in lipid membranes and cell membranes is of interest to biology because of the idea of membrane rafts — compositionally heterogeneous liquid crystal domains with cellular functions. Few quantitative tools exist for characterizing and differentiating coexisting phases on a molecular scale. Lipid acyl chain order can be measured directly using deuterium nuclear magnetic resonance spectroscopy (2H NMR), or inferred using fluorescence microscopy along with the environment-sensitive probe Laurdan. We found a linear relationship between the 2H NMR order parameter and Laurdan generalized polarization. This observed correlation supports the idea that lipid chain order is tightly associated with the amount and dynamics of water molecules at the glycerol backbone level of the membrane.

OriginalsprogEngelsk
Artikelnummer183053
TidsskriftBiochimica et Biophysica Acta - Biomembranes
Vol/bind1861
Udgave nummer12
Antal sider8
ISSN0005-2736
DOI
StatusUdgivet - 1. dec. 2019

Fingeraftryk

Deuterium
Membrane Lipids
Nuclear magnetic resonance
Polarization
Membranes
Lipids
Liquid Crystals
Fluorescence microscopy
Cell membranes
Fluorescence Microscopy
Phase separation
Glycerol
Nuclear magnetic resonance spectroscopy
Cell Membrane
Molecules
Water
laurdan

Citer dette

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title = "Measuring molecular order for lipid membrane phase studies: Linear relationship between Laurdan generalized polarization and deuterium NMR order parameter",
abstract = "Two dimensional phase separation in lipid membranes and cell membranes is of interest to biology because of the idea of membrane rafts — compositionally heterogeneous liquid crystal domains with cellular functions. Few quantitative tools exist for characterizing and differentiating coexisting phases on a molecular scale. Lipid acyl chain order can be measured directly using deuterium nuclear magnetic resonance spectroscopy (2H NMR), or inferred using fluorescence microscopy along with the environment-sensitive probe Laurdan. We found a linear relationship between the 2H NMR order parameter and Laurdan generalized polarization. This observed correlation supports the idea that lipid chain order is tightly associated with the amount and dynamics of water molecules at the glycerol backbone level of the membrane.",
keywords = "Deuterium nuclear magnetic resonance spectroscopy, Fluorescence microscopy, Laurdan, Lipid model membrane, Liquid disordered-liquid ordered coexistence, Membrane order",
author = "Leung, {Sherry See Wai} and Jonathan Brewer and Bagatolli, {Luis A.} and Thewalt, {Jenifer L.}",
year = "2019",
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journal = "B B A - Biomembranes",
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Measuring molecular order for lipid membrane phase studies : Linear relationship between Laurdan generalized polarization and deuterium NMR order parameter. / Leung, Sherry See Wai; Brewer, Jonathan; Bagatolli, Luis A.; Thewalt, Jenifer L.

I: Biochimica et Biophysica Acta - Biomembranes, Bind 1861, Nr. 12, 183053, 01.12.2019.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Measuring molecular order for lipid membrane phase studies

T2 - Linear relationship between Laurdan generalized polarization and deuterium NMR order parameter

AU - Leung, Sherry See Wai

AU - Brewer, Jonathan

AU - Bagatolli, Luis A.

AU - Thewalt, Jenifer L.

PY - 2019/12/1

Y1 - 2019/12/1

N2 - Two dimensional phase separation in lipid membranes and cell membranes is of interest to biology because of the idea of membrane rafts — compositionally heterogeneous liquid crystal domains with cellular functions. Few quantitative tools exist for characterizing and differentiating coexisting phases on a molecular scale. Lipid acyl chain order can be measured directly using deuterium nuclear magnetic resonance spectroscopy (2H NMR), or inferred using fluorescence microscopy along with the environment-sensitive probe Laurdan. We found a linear relationship between the 2H NMR order parameter and Laurdan generalized polarization. This observed correlation supports the idea that lipid chain order is tightly associated with the amount and dynamics of water molecules at the glycerol backbone level of the membrane.

AB - Two dimensional phase separation in lipid membranes and cell membranes is of interest to biology because of the idea of membrane rafts — compositionally heterogeneous liquid crystal domains with cellular functions. Few quantitative tools exist for characterizing and differentiating coexisting phases on a molecular scale. Lipid acyl chain order can be measured directly using deuterium nuclear magnetic resonance spectroscopy (2H NMR), or inferred using fluorescence microscopy along with the environment-sensitive probe Laurdan. We found a linear relationship between the 2H NMR order parameter and Laurdan generalized polarization. This observed correlation supports the idea that lipid chain order is tightly associated with the amount and dynamics of water molecules at the glycerol backbone level of the membrane.

KW - Deuterium nuclear magnetic resonance spectroscopy

KW - Fluorescence microscopy

KW - Laurdan

KW - Lipid model membrane

KW - Liquid disordered-liquid ordered coexistence

KW - Membrane order

U2 - 10.1016/j.bbamem.2019.183053

DO - 10.1016/j.bbamem.2019.183053

M3 - Journal article

VL - 1861

JO - B B A - Biomembranes

JF - B B A - Biomembranes

SN - 0005-2736

IS - 12

M1 - 183053

ER -