Measurement of total CO2 in microliter samples of urine and other biological fluids using infrared detection of CO2

Francesco  Trepiccione; Francesco Maria Iena; Laura Catalini; Francesco Martino  Carpi; Mogens  Koed; Sebastian  Frische

doi:10.1007/s00424-017-1997-8

Measurement of total CO2 in microliter samples of urine and other biological fluids using infrared detection of CO2

Francesco Trepiccione, Francesco Maria Iena, Laura Catalini, Francesco Martino Carpi, Mogens Koed, Sebastian Frische^*

^*Kontaktforfatter

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

Abstrakt

The purpose of this study is to describe a low-cost and simply made instrument capable of measuring the total CO ₂ content of microliter volumes of biological fluids utilizing a commercially available CO ₂ sensor based on a NDIR detector. The described instrument is based on transformation of dissolved HCO ₃ ⁻ to CO ₂ by acidification and subsequent measurement of the produced CO ₂. The instrument has a linear response in the range 0.025–10 μmol HCO ₃ ⁻, which enables measurements in fresh urine and plasma samples down to 5 μl. The values from plasma were compared to measurements made on 65 μl whole blood in an automatic blood gas analyzer and found not to differ significantly. Compared to currently commercially available instruments applying the same principles to measure total CO ₂, this study provides a simple and robust alternative which even can be used on smaller sample volumes.

Originalsprog	Engelsk
Tidsskrift	Pflügers Archiv - European Journal of Physiology
Vol/bind	469
Udgave nummer	10
Sider (fra-til)	1267-1275
ISSN	0031-6768
DOI	https://doi.org/10.1007/s00424-017-1997-8
Status	Udgivet - 1. okt. 2017
Udgivet eksternt	Ja

Dokumenter og links

10.1007/s00424-017-1997-8

Citationsformater

@article{e9ada26400cd4563ae12aed7c10f8146,

title = "Measurement of total CO2 in microliter samples of urine and other biological fluids using infrared detection of CO2",

abstract = "The purpose of this study is to describe a low-cost and simply made instrument capable of measuring the total CO 2 content of microliter volumes of biological fluids utilizing a commercially available CO 2 sensor based on a NDIR detector. The described instrument is based on transformation of dissolved HCO 3 − to CO 2 by acidification and subsequent measurement of the produced CO 2. The instrument has a linear response in the range 0.025–10 μmol HCO 3 −, which enables measurements in fresh urine and plasma samples down to 5 μl. The values from plasma were compared to measurements made on 65 μl whole blood in an automatic blood gas analyzer and found not to differ significantly. Compared to currently commercially available instruments applying the same principles to measure total CO 2, this study provides a simple and robust alternative which even can be used on smaller sample volumes. ",

keywords = "Acid/base status, Bicarbonate, NDIR, Total CO, Urine",

author = "Francesco Trepiccione and Iena, {Francesco Maria} and Laura Catalini and Carpi, {Francesco Martino} and Mogens Koed and Sebastian Frische",

year = "2017",

month = oct,

day = "1",

doi = "10.1007/s00424-017-1997-8",

language = "English",

volume = "469",

pages = "1267--1275",

journal = "Pfl{\"u}gers Archiv - European Journal of Physiology",

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Measurement of total CO2 in microliter samples of urine and other biological fluids using infrared detection of CO2. / Trepiccione, Francesco ; Iena, Francesco Maria; Catalini, Laura et al.

I: Pflügers Archiv - European Journal of Physiology, Bind 469, Nr. 10, 01.10.2017, s. 1267-1275.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

TY - JOUR

T1 - Measurement of total CO2 in microliter samples of urine and other biological fluids using infrared detection of CO2

AU - Trepiccione, Francesco

AU - Iena, Francesco Maria

AU - Catalini, Laura

AU - Carpi, Francesco Martino

AU - Koed, Mogens

AU - Frische, Sebastian

PY - 2017/10/1

Y1 - 2017/10/1

N2 - The purpose of this study is to describe a low-cost and simply made instrument capable of measuring the total CO 2 content of microliter volumes of biological fluids utilizing a commercially available CO 2 sensor based on a NDIR detector. The described instrument is based on transformation of dissolved HCO 3 − to CO 2 by acidification and subsequent measurement of the produced CO 2. The instrument has a linear response in the range 0.025–10 μmol HCO 3 −, which enables measurements in fresh urine and plasma samples down to 5 μl. The values from plasma were compared to measurements made on 65 μl whole blood in an automatic blood gas analyzer and found not to differ significantly. Compared to currently commercially available instruments applying the same principles to measure total CO 2, this study provides a simple and robust alternative which even can be used on smaller sample volumes.

AB - The purpose of this study is to describe a low-cost and simply made instrument capable of measuring the total CO 2 content of microliter volumes of biological fluids utilizing a commercially available CO 2 sensor based on a NDIR detector. The described instrument is based on transformation of dissolved HCO 3 − to CO 2 by acidification and subsequent measurement of the produced CO 2. The instrument has a linear response in the range 0.025–10 μmol HCO 3 −, which enables measurements in fresh urine and plasma samples down to 5 μl. The values from plasma were compared to measurements made on 65 μl whole blood in an automatic blood gas analyzer and found not to differ significantly. Compared to currently commercially available instruments applying the same principles to measure total CO 2, this study provides a simple and robust alternative which even can be used on smaller sample volumes.

KW - Acid/base status

KW - Bicarbonate

KW - NDIR

KW - Total CO

KW - Urine

U2 - 10.1007/s00424-017-1997-8

DO - 10.1007/s00424-017-1997-8

M3 - Journal article

VL - 469

SP - 1267

EP - 1275

JO - Pflügers Archiv - European Journal of Physiology

JF - Pflügers Archiv - European Journal of Physiology

SN - 0031-6768

IS - 10

ER -

Measurement of total CO2 in microliter samples of urine and other biological fluids using infrared detection of CO2

Abstrakt

Dokumenter og links

Fingeraftryk

Citationsformater