Mass spectrometry in epigenetic research

Hans Christian Beck

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

Resumé

The inhibition of the histone deacetylase enzymes induces hyperacetylation of the histone proteins. This hyperacetylation causes cell cycle arrest and cell death in cancer cells but not in normal cells. Therefore, the development of histone deacetylase inhibitors for the treatment of various cancers has gained tremendous interest in recent years, and many of these inhibitors are currently undergoing clinical trials. Despite intense research, however, the exact molecular mechanisms of action of these molecules remain, to a wide extent, unclear. The recent application of mass spectrometry-based proteomics techniques to histone biology has gained new insight into the function of the nucleosome: Novel posttranslational modifications have been discovered at the lateral surface of the nucleosome. These modifications regulate histone-DNA interactions, adding a new dimension to the epigenetic regulation of nucleosome mobility.
OriginalsprogEngelsk
TidsskriftMethods in Molecular Biology
Vol/bind593
Sider (fra-til)263-82
Antal sider20
ISSN1064-3745
DOI
StatusUdgivet - 2010

Fingeraftryk

Nucleosomes
Epigenomics
Histones
Research
Histone Deacetylase Inhibitors
Histone Deacetylases
Cell Cycle Checkpoints
Neoplasms
Cell Death
Clinical Trials
DNA
Enzymes
Proteins

Citer dette

Beck, Hans Christian. / Mass spectrometry in epigenetic research. I: Methods in Molecular Biology. 2010 ; Bind 593. s. 263-82.
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abstract = "The inhibition of the histone deacetylase enzymes induces hyperacetylation of the histone proteins. This hyperacetylation causes cell cycle arrest and cell death in cancer cells but not in normal cells. Therefore, the development of histone deacetylase inhibitors for the treatment of various cancers has gained tremendous interest in recent years, and many of these inhibitors are currently undergoing clinical trials. Despite intense research, however, the exact molecular mechanisms of action of these molecules remain, to a wide extent, unclear. The recent application of mass spectrometry-based proteomics techniques to histone biology has gained new insight into the function of the nucleosome: Novel posttranslational modifications have been discovered at the lateral surface of the nucleosome. These modifications regulate histone-DNA interactions, adding a new dimension to the epigenetic regulation of nucleosome mobility.",
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Beck, HC 2010, 'Mass spectrometry in epigenetic research', Methods in Molecular Biology, bind 593, s. 263-82. https://doi.org/10.1007/978-1-60327-194-3_13

Mass spectrometry in epigenetic research. / Beck, Hans Christian.

I: Methods in Molecular Biology, Bind 593, 2010, s. 263-82.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

TY - JOUR

T1 - Mass spectrometry in epigenetic research

AU - Beck, Hans Christian

PY - 2010

Y1 - 2010

N2 - The inhibition of the histone deacetylase enzymes induces hyperacetylation of the histone proteins. This hyperacetylation causes cell cycle arrest and cell death in cancer cells but not in normal cells. Therefore, the development of histone deacetylase inhibitors for the treatment of various cancers has gained tremendous interest in recent years, and many of these inhibitors are currently undergoing clinical trials. Despite intense research, however, the exact molecular mechanisms of action of these molecules remain, to a wide extent, unclear. The recent application of mass spectrometry-based proteomics techniques to histone biology has gained new insight into the function of the nucleosome: Novel posttranslational modifications have been discovered at the lateral surface of the nucleosome. These modifications regulate histone-DNA interactions, adding a new dimension to the epigenetic regulation of nucleosome mobility.

AB - The inhibition of the histone deacetylase enzymes induces hyperacetylation of the histone proteins. This hyperacetylation causes cell cycle arrest and cell death in cancer cells but not in normal cells. Therefore, the development of histone deacetylase inhibitors for the treatment of various cancers has gained tremendous interest in recent years, and many of these inhibitors are currently undergoing clinical trials. Despite intense research, however, the exact molecular mechanisms of action of these molecules remain, to a wide extent, unclear. The recent application of mass spectrometry-based proteomics techniques to histone biology has gained new insight into the function of the nucleosome: Novel posttranslational modifications have been discovered at the lateral surface of the nucleosome. These modifications regulate histone-DNA interactions, adding a new dimension to the epigenetic regulation of nucleosome mobility.

KW - Animals

KW - Drug Screening Assays, Antitumor

KW - Epigenesis, Genetic

KW - Histone Deacetylase Inhibitors

KW - Histone Deacetylases

KW - Histones

KW - Humans

KW - Mass Spectrometry

KW - Neoplasms

KW - Protein Processing, Post-Translational

KW - Proteomics

KW - Research

U2 - 10.1007/978-1-60327-194-3_13

DO - 10.1007/978-1-60327-194-3_13

M3 - Journal article

VL - 593

SP - 263

EP - 282

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

SN - 1064-3745

ER -

Beck HC. Mass spectrometry in epigenetic research. Methods in Molecular Biology. 2010;593:263-82. https://doi.org/10.1007/978-1-60327-194-3_13

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