Low hepatitis B prevalence among pre-school children in Denmark: saliva anti-HBc screening in day care centres

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Although Denmark has a low hepatitis B virus (HBV) prevalence, HBV transmission has been reported in Danish day-care centres. The aim of this study was to validate saliva anti-HBc testing as a method for HBV screening, the applicability of saliva sampling to pre-school children, and to determine the HBV prevalence in Danish day-care centres with a high proportion of immigrants. For validation, paired saliva and plasma samples were obtained from blood donors and injecting drug users. Employees and children in day-care centres with a high proportion of immigrant children were offered saliva screening followed by blood test if positive. The specificity and sensitivity of anti-HBc tests on saliva was 100% (102 blood donors and four injecting drug users) and 85.9% (61 of 71 anti-HBc-positive injecting drug users), respectively. In all samples from HBsAg (n = 7) or anti-HBc IgM-positives (n = 9), anti-HBc was detected in saliva. Adequate saliva samples were obtained from 93% (588/634) of children and 100% (166/166) of employees participating in the day-care centre survey. Among children 55% were of non-Scandinavian origin and only one (0.2%, 95% CI [0.0; 1.0]) was HBV positive. Among employees the corresponding values were 22% and 7 (4.2%). The positive predictive value of the saliva test was 25% (1/4) among children and 88% (7/8) among adults. In conclusion, saliva testing is feasible for HBV screening among children in low prevalence populations, but any anti-HBc reactivity should be confirmed by plasma analysis. The HBV prevalence in pre-school children in Denmark is low even among immigrants from endemic areas.

OriginalsprogEngelsk
TidsskriftJournal of Medical Virology
Vol/bind68
Udgave nummer4
Sider (fra-til)500-504
ISSN0146-6615
DOI
StatusUdgivet - 2002

Fingeraftryk

Denmark
Hepatitis B
Saliva
Hepatitis B virus
Drug Users
Child Day Care Centers
Predictive Value of Tests
Hepatitis B Surface Antigens

Citer dette

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title = "Low hepatitis B prevalence among pre-school children in Denmark: saliva anti-HBc screening in day care centres",
abstract = "Although Denmark has a low hepatitis B virus (HBV) prevalence, HBV transmission has been reported in Danish day-care centres. The aim of this study was to validate saliva anti-HBc testing as a method for HBV screening, the applicability of saliva sampling to pre-school children, and to determine the HBV prevalence in Danish day-care centres with a high proportion of immigrants. For validation, paired saliva and plasma samples were obtained from blood donors and injecting drug users. Employees and children in day-care centres with a high proportion of immigrant children were offered saliva screening followed by blood test if positive. The specificity and sensitivity of anti-HBc tests on saliva was 100{\%} (102 blood donors and four injecting drug users) and 85.9{\%} (61 of 71 anti-HBc-positive injecting drug users), respectively. In all samples from HBsAg (n = 7) or anti-HBc IgM-positives (n = 9), anti-HBc was detected in saliva. Adequate saliva samples were obtained from 93{\%} (588/634) of children and 100{\%} (166/166) of employees participating in the day-care centre survey. Among children 55{\%} were of non-Scandinavian origin and only one (0.2{\%}, 95{\%} CI [0.0; 1.0]) was HBV positive. Among employees the corresponding values were 22{\%} and 7 (4.2{\%}). The positive predictive value of the saliva test was 25{\%} (1/4) among children and 88{\%} (7/8) among adults. In conclusion, saliva testing is feasible for HBV screening among children in low prevalence populations, but any anti-HBc reactivity should be confirmed by plasma analysis. The HBV prevalence in pre-school children in Denmark is low even among immigrants from endemic areas.",
keywords = "Child, Child Day Care Centers, Child, Preschool, Data Collection, Denmark, Hepatitis B, Hepatitis B Antibodies, Hepatitis B Core Antigens, Hepatitis B Surface Antigens, Humans, Saliva, Seroepidemiologic Studies",
author = "Niels Fisker and J{\o}rgen Georgsen and Torsten Stolborg and Mohammad Khalil and Christensen, {Peer Brehm}",
note = "Copyright 2002 Wiley-Liss, Inc.",
year = "2002",
doi = "10.1002/jmv.10242",
language = "English",
volume = "68",
pages = "500--504",
journal = "Journal of Medical Virology",
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Low hepatitis B prevalence among pre-school children in Denmark : saliva anti-HBc screening in day care centres. / Fisker, Niels; Georgsen, Jørgen; Stolborg, Torsten; Khalil, Mohammad; Christensen, Peer Brehm.

I: Journal of Medical Virology, Bind 68, Nr. 4, 2002, s. 500-504.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Low hepatitis B prevalence among pre-school children in Denmark

T2 - saliva anti-HBc screening in day care centres

AU - Fisker, Niels

AU - Georgsen, Jørgen

AU - Stolborg, Torsten

AU - Khalil, Mohammad

AU - Christensen, Peer Brehm

N1 - Copyright 2002 Wiley-Liss, Inc.

PY - 2002

Y1 - 2002

N2 - Although Denmark has a low hepatitis B virus (HBV) prevalence, HBV transmission has been reported in Danish day-care centres. The aim of this study was to validate saliva anti-HBc testing as a method for HBV screening, the applicability of saliva sampling to pre-school children, and to determine the HBV prevalence in Danish day-care centres with a high proportion of immigrants. For validation, paired saliva and plasma samples were obtained from blood donors and injecting drug users. Employees and children in day-care centres with a high proportion of immigrant children were offered saliva screening followed by blood test if positive. The specificity and sensitivity of anti-HBc tests on saliva was 100% (102 blood donors and four injecting drug users) and 85.9% (61 of 71 anti-HBc-positive injecting drug users), respectively. In all samples from HBsAg (n = 7) or anti-HBc IgM-positives (n = 9), anti-HBc was detected in saliva. Adequate saliva samples were obtained from 93% (588/634) of children and 100% (166/166) of employees participating in the day-care centre survey. Among children 55% were of non-Scandinavian origin and only one (0.2%, 95% CI [0.0; 1.0]) was HBV positive. Among employees the corresponding values were 22% and 7 (4.2%). The positive predictive value of the saliva test was 25% (1/4) among children and 88% (7/8) among adults. In conclusion, saliva testing is feasible for HBV screening among children in low prevalence populations, but any anti-HBc reactivity should be confirmed by plasma analysis. The HBV prevalence in pre-school children in Denmark is low even among immigrants from endemic areas.

AB - Although Denmark has a low hepatitis B virus (HBV) prevalence, HBV transmission has been reported in Danish day-care centres. The aim of this study was to validate saliva anti-HBc testing as a method for HBV screening, the applicability of saliva sampling to pre-school children, and to determine the HBV prevalence in Danish day-care centres with a high proportion of immigrants. For validation, paired saliva and plasma samples were obtained from blood donors and injecting drug users. Employees and children in day-care centres with a high proportion of immigrant children were offered saliva screening followed by blood test if positive. The specificity and sensitivity of anti-HBc tests on saliva was 100% (102 blood donors and four injecting drug users) and 85.9% (61 of 71 anti-HBc-positive injecting drug users), respectively. In all samples from HBsAg (n = 7) or anti-HBc IgM-positives (n = 9), anti-HBc was detected in saliva. Adequate saliva samples were obtained from 93% (588/634) of children and 100% (166/166) of employees participating in the day-care centre survey. Among children 55% were of non-Scandinavian origin and only one (0.2%, 95% CI [0.0; 1.0]) was HBV positive. Among employees the corresponding values were 22% and 7 (4.2%). The positive predictive value of the saliva test was 25% (1/4) among children and 88% (7/8) among adults. In conclusion, saliva testing is feasible for HBV screening among children in low prevalence populations, but any anti-HBc reactivity should be confirmed by plasma analysis. The HBV prevalence in pre-school children in Denmark is low even among immigrants from endemic areas.

KW - Child

KW - Child Day Care Centers

KW - Child, Preschool

KW - Data Collection

KW - Denmark

KW - Hepatitis B

KW - Hepatitis B Antibodies

KW - Hepatitis B Core Antigens

KW - Hepatitis B Surface Antigens

KW - Humans

KW - Saliva

KW - Seroepidemiologic Studies

U2 - 10.1002/jmv.10242

DO - 10.1002/jmv.10242

M3 - Journal article

C2 - 12376957

VL - 68

SP - 500

EP - 504

JO - Journal of Medical Virology

JF - Journal of Medical Virology

SN - 0146-6615

IS - 4

ER -