Longevity is independent of common variations in genes associated with cardiovascular risk.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

 
Udgivelsesdato: 1999-Sep
OriginalsprogEngelsk
TidsskriftThrombosis and Haemostasis
Vol/bind82
Udgave nummer3
Sider (fra-til)1100-1105
Antal sider5
ISSN0340-6245
StatusUdgivet - 1. sep. 1999

Fingeraftryk

Introns
Plasminogen Activator Inhibitor 1
Prothrombin
Alleles
Platelet Glycoprotein GPIIb-IIIa Complex
DNA
Tissue Plasminogen Activator
Peptidyl-Dipeptidase A
Restriction Fragment Length Polymorphisms
Polymerase Chain Reaction

Citer dette

@article{ac62a3d0ec1b11dc86ef000ea68e967b,
title = "Longevity is independent of common variations in genes associated with cardiovascular risk.",
abstract = "Do extremely old persons have a genetically favourable profile which has protected them from cardiovascular death? We have tried to answer this question by measuring DNA polymorphisms of selected cardiovascular risk indicators [factor VII, FVII (R/Q353, intron 7 (37bp)n, and -323ins10), beta fibrinogen (-455G/A), plasminogen activator inhibitor type 1, PAI-1 (-675(4G/5G)), tissue plasminogen activator, t-PA (intron 8 ins311), platelet receptor glycoprotein IIb/IIIa, GPIIb/IIIa (L/P33), prothrombin (20210G/A), methylene tetrahydrofolate reductase, MTHFR (A/V114), angiotensin converting enzyme, ACE (intron 16 ins287), and angiotensinogen (M/T235)]. Blood was collected from 187 unselected Danish centenarians, and 201 healthy Danish blood donors, aged 20-64 years (mean age 42 years). Genomic DNA was amplified using PCR and the genotype was determined by RFLP methods or allele-specific amplification followed by agarose gel electrophoresis. The frequencies of the high-risk alleles in centenarians were: for FVII R/Q353 0.91; for FVII intron 7 (37bp)n 0.67; for FVII-323 ins10 0.90; for fibrinogen 0.16; for PAI-1 0.52; for t-PA 0.59; for GPIIb/IIIa 0.16; for prothrombin 0.008; for MTHFR 0.33; for ACE 0.52; and for angiotensinogen 0.36. Comparable frequencies were observed in the blood donors. Subgroup analysis of men and women separately gave similar results. The genotype frequencies in the centenarians and the blood donors were similar for all polymorphisms, and this study suggests that common variations in genes associated with cardiovascular risk do not contribute significantly to longevity.",
keywords = "Adult, Aged, Aged, 80 and over, Alleles, Blood Pressure, Cardiovascular Diseases, DNA, Denmark, Female, Gene Frequency, Hemostasis, Humans, Longevity, Male, Middle Aged, Polymorphism, Genetic, Risk Factors, Variation (Genetics)",
author = "Bladbjerg, {E M} and K Andersen-Ranberg and Maat, {M de} and Kristensen, {S R} and B Jeune and J Gram and J Jespersen",
year = "1999",
month = "9",
day = "1",
language = "English",
volume = "82",
pages = "1100--1105",
journal = "Thrombosis and Haemostasis",
issn = "0340-6245",
publisher = "Schattauer",
number = "3",

}

Longevity is independent of common variations in genes associated with cardiovascular risk. / Bladbjerg, E M; Andersen-Ranberg, K; Maat, M de; Kristensen, S R; Jeune, B; Gram, J; Jespersen, J.

I: Thrombosis and Haemostasis, Bind 82, Nr. 3, 01.09.1999, s. 1100-1105.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Longevity is independent of common variations in genes associated with cardiovascular risk.

AU - Bladbjerg, E M

AU - Andersen-Ranberg, K

AU - Maat, M de

AU - Kristensen, S R

AU - Jeune, B

AU - Gram, J

AU - Jespersen, J

PY - 1999/9/1

Y1 - 1999/9/1

N2 - Do extremely old persons have a genetically favourable profile which has protected them from cardiovascular death? We have tried to answer this question by measuring DNA polymorphisms of selected cardiovascular risk indicators [factor VII, FVII (R/Q353, intron 7 (37bp)n, and -323ins10), beta fibrinogen (-455G/A), plasminogen activator inhibitor type 1, PAI-1 (-675(4G/5G)), tissue plasminogen activator, t-PA (intron 8 ins311), platelet receptor glycoprotein IIb/IIIa, GPIIb/IIIa (L/P33), prothrombin (20210G/A), methylene tetrahydrofolate reductase, MTHFR (A/V114), angiotensin converting enzyme, ACE (intron 16 ins287), and angiotensinogen (M/T235)]. Blood was collected from 187 unselected Danish centenarians, and 201 healthy Danish blood donors, aged 20-64 years (mean age 42 years). Genomic DNA was amplified using PCR and the genotype was determined by RFLP methods or allele-specific amplification followed by agarose gel electrophoresis. The frequencies of the high-risk alleles in centenarians were: for FVII R/Q353 0.91; for FVII intron 7 (37bp)n 0.67; for FVII-323 ins10 0.90; for fibrinogen 0.16; for PAI-1 0.52; for t-PA 0.59; for GPIIb/IIIa 0.16; for prothrombin 0.008; for MTHFR 0.33; for ACE 0.52; and for angiotensinogen 0.36. Comparable frequencies were observed in the blood donors. Subgroup analysis of men and women separately gave similar results. The genotype frequencies in the centenarians and the blood donors were similar for all polymorphisms, and this study suggests that common variations in genes associated with cardiovascular risk do not contribute significantly to longevity.

AB - Do extremely old persons have a genetically favourable profile which has protected them from cardiovascular death? We have tried to answer this question by measuring DNA polymorphisms of selected cardiovascular risk indicators [factor VII, FVII (R/Q353, intron 7 (37bp)n, and -323ins10), beta fibrinogen (-455G/A), plasminogen activator inhibitor type 1, PAI-1 (-675(4G/5G)), tissue plasminogen activator, t-PA (intron 8 ins311), platelet receptor glycoprotein IIb/IIIa, GPIIb/IIIa (L/P33), prothrombin (20210G/A), methylene tetrahydrofolate reductase, MTHFR (A/V114), angiotensin converting enzyme, ACE (intron 16 ins287), and angiotensinogen (M/T235)]. Blood was collected from 187 unselected Danish centenarians, and 201 healthy Danish blood donors, aged 20-64 years (mean age 42 years). Genomic DNA was amplified using PCR and the genotype was determined by RFLP methods or allele-specific amplification followed by agarose gel electrophoresis. The frequencies of the high-risk alleles in centenarians were: for FVII R/Q353 0.91; for FVII intron 7 (37bp)n 0.67; for FVII-323 ins10 0.90; for fibrinogen 0.16; for PAI-1 0.52; for t-PA 0.59; for GPIIb/IIIa 0.16; for prothrombin 0.008; for MTHFR 0.33; for ACE 0.52; and for angiotensinogen 0.36. Comparable frequencies were observed in the blood donors. Subgroup analysis of men and women separately gave similar results. The genotype frequencies in the centenarians and the blood donors were similar for all polymorphisms, and this study suggests that common variations in genes associated with cardiovascular risk do not contribute significantly to longevity.

KW - Adult

KW - Aged

KW - Aged, 80 and over

KW - Alleles

KW - Blood Pressure

KW - Cardiovascular Diseases

KW - DNA

KW - Denmark

KW - Female

KW - Gene Frequency

KW - Hemostasis

KW - Humans

KW - Longevity

KW - Male

KW - Middle Aged

KW - Polymorphism, Genetic

KW - Risk Factors

KW - Variation (Genetics)

M3 - Journal article

VL - 82

SP - 1100

EP - 1105

JO - Thrombosis and Haemostasis

JF - Thrombosis and Haemostasis

SN - 0340-6245

IS - 3

ER -