Locked nucleic acid (LNA): High affinity targeting of RNA for diagnostics and therapeutics

S. Kauppinen, Birte Vester, Jesper Wengel

Publikation: Bidrag til tidsskriftReviewForskning

Resumé

Locked nucleic acid (LNA) is a nucleic acid analogue
containing one or more LNA nucleotide monomers
with a bicyclic furanose unit locked in an RNA mimicking
sugar conformation. This conformational restriction
results in unprecedented hybridization affinity
towards complementary single stranded RNA and
thus, makes LNA uniquely suited for mimicking RNA
structures and sequence specific targeting of RNA in
vitro or in vivo. The focus of this paper is on LNAantisense,
LNA-modified siRNA (siLNA), and detection
and analysis of microRNAs by LNA-modified oligonucleotide
probes.
OriginalsprogEngelsk
TidsskriftDrug Discovery Today
Vol/bind2
Udgave nummer3
Sider (fra-til)287-290
Antal sider4
ISSN1359-6446
DOI
StatusUdgivet - 2005

Fingeraftryk

RNA
MicroRNAs
Nucleic Acids
Small Interfering RNA

Citer dette

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Locked nucleic acid (LNA): High affinity targeting of RNA for diagnostics and therapeutics. / Kauppinen, S.; Vester, Birte; Wengel, Jesper.

I: Drug Discovery Today, Bind 2, Nr. 3, 2005, s. 287-290.

Publikation: Bidrag til tidsskriftReviewForskning

TY - JOUR

T1 - Locked nucleic acid (LNA): High affinity targeting of RNA for diagnostics and therapeutics

AU - Kauppinen, S.

AU - Vester, Birte

AU - Wengel, Jesper

PY - 2005

Y1 - 2005

N2 - Locked nucleic acid (LNA) is a nucleic acid analoguecontaining one or more LNA nucleotide monomerswith a bicyclic furanose unit locked in an RNA mimickingsugar conformation. This conformational restrictionresults in unprecedented hybridization affinitytowards complementary single stranded RNA andthus, makes LNA uniquely suited for mimicking RNAstructures and sequence specific targeting of RNA invitro or in vivo. The focus of this paper is on LNAantisense,LNA-modified siRNA (siLNA), and detectionand analysis of microRNAs by LNA-modified oligonucleotideprobes.

AB - Locked nucleic acid (LNA) is a nucleic acid analoguecontaining one or more LNA nucleotide monomerswith a bicyclic furanose unit locked in an RNA mimickingsugar conformation. This conformational restrictionresults in unprecedented hybridization affinitytowards complementary single stranded RNA andthus, makes LNA uniquely suited for mimicking RNAstructures and sequence specific targeting of RNA invitro or in vivo. The focus of this paper is on LNAantisense,LNA-modified siRNA (siLNA), and detectionand analysis of microRNAs by LNA-modified oligonucleotideprobes.

U2 - 10.1016/j.ddtec.2005.08.012

DO - 10.1016/j.ddtec.2005.08.012

M3 - Review

VL - 2

SP - 287

EP - 290

JO - Drug Discovery Today

JF - Drug Discovery Today

SN - 1359-6446

IS - 3

ER -