LNA (locked nucleic acid) and analogs as triplex-forming oligonucleotides

Torben Højland, Surender Kumar, Bolle Ravindra Babu, Tadashi Umemoto, Nanna Albaek, Pawan K Sharma, Poul Nielsen, Jesper Wengel

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

 
Udgivelsesdato: 2007-Aug-7
OriginalsprogEngelsk
TidsskriftOrganic & Biomolecular Chemistry
Vol/bind5
Udgave nummer15
Sider (fra-til)2375-9
Antal sider4
ISSN1477-0520
DOI
StatusUdgivet - 2007

Fingeraftryk

Oligonucleotides
Monomers
Conformations
Nucleotides
locked nucleic acid
propylene

Citer dette

Højland, Torben ; Kumar, Surender ; Babu, Bolle Ravindra ; Umemoto, Tadashi ; Albaek, Nanna ; Sharma, Pawan K ; Nielsen, Poul ; Wengel, Jesper. / LNA (locked nucleic acid) and analogs as triplex-forming oligonucleotides. I: Organic & Biomolecular Chemistry. 2007 ; Bind 5, Nr. 15. s. 2375-9.
@article{d806dc20df9d11dca57f000ea68e967b,
title = "LNA (locked nucleic acid) and analogs as triplex-forming oligonucleotides",
abstract = "The triplex-forming abilities of some conformationally restricted nucleotide analogs are disclosed and compared herein. 2'-Amino-LNA monomers proved to be less stabilising to triplexes than LNA monomers when incorporated into a triplex-forming third strand. N2'-functionalisation of 2'-amino-LNA monomers with a glycyl unit induced the formation of exceptionally stable triplexes. Nucleotide analogs containing a C2',C3'-oxymethylene linker (E-type furanose conformation) or a C2',C4'-propylene linker (N-type furanose conformation) had no significant effect on triplex stability proving that conformational restriction per se is insufficient to stabilise triplexes.",
keywords = "DNA, Hydrogen Bonding, Nucleic Acid Conformation, Oligonucleotides, Oligonucleotides, Antisense, Transition Temperature",
author = "Torben H{\o}jland and Surender Kumar and Babu, {Bolle Ravindra} and Tadashi Umemoto and Nanna Albaek and Sharma, {Pawan K} and Poul Nielsen and Jesper Wengel",
year = "2007",
doi = "10.1039/b706101c",
language = "English",
volume = "5",
pages = "2375--9",
journal = "Organic & Biomolecular Chemistry",
issn = "1477-0520",
publisher = "Royal Society of Chemistry",
number = "15",

}

LNA (locked nucleic acid) and analogs as triplex-forming oligonucleotides. / Højland, Torben; Kumar, Surender; Babu, Bolle Ravindra; Umemoto, Tadashi; Albaek, Nanna; Sharma, Pawan K; Nielsen, Poul; Wengel, Jesper.

I: Organic & Biomolecular Chemistry, Bind 5, Nr. 15, 2007, s. 2375-9.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - LNA (locked nucleic acid) and analogs as triplex-forming oligonucleotides

AU - Højland, Torben

AU - Kumar, Surender

AU - Babu, Bolle Ravindra

AU - Umemoto, Tadashi

AU - Albaek, Nanna

AU - Sharma, Pawan K

AU - Nielsen, Poul

AU - Wengel, Jesper

PY - 2007

Y1 - 2007

N2 - The triplex-forming abilities of some conformationally restricted nucleotide analogs are disclosed and compared herein. 2'-Amino-LNA monomers proved to be less stabilising to triplexes than LNA monomers when incorporated into a triplex-forming third strand. N2'-functionalisation of 2'-amino-LNA monomers with a glycyl unit induced the formation of exceptionally stable triplexes. Nucleotide analogs containing a C2',C3'-oxymethylene linker (E-type furanose conformation) or a C2',C4'-propylene linker (N-type furanose conformation) had no significant effect on triplex stability proving that conformational restriction per se is insufficient to stabilise triplexes.

AB - The triplex-forming abilities of some conformationally restricted nucleotide analogs are disclosed and compared herein. 2'-Amino-LNA monomers proved to be less stabilising to triplexes than LNA monomers when incorporated into a triplex-forming third strand. N2'-functionalisation of 2'-amino-LNA monomers with a glycyl unit induced the formation of exceptionally stable triplexes. Nucleotide analogs containing a C2',C3'-oxymethylene linker (E-type furanose conformation) or a C2',C4'-propylene linker (N-type furanose conformation) had no significant effect on triplex stability proving that conformational restriction per se is insufficient to stabilise triplexes.

KW - DNA

KW - Hydrogen Bonding

KW - Nucleic Acid Conformation

KW - Oligonucleotides

KW - Oligonucleotides, Antisense

KW - Transition Temperature

U2 - 10.1039/b706101c

DO - 10.1039/b706101c

M3 - Journal article

VL - 5

SP - 2375

EP - 2379

JO - Organic & Biomolecular Chemistry

JF - Organic & Biomolecular Chemistry

SN - 1477-0520

IS - 15

ER -