Mixmer LNA/DNA fluorescent probes containing the 1-(phenylethynyl)pyrene fluorophore attached to 2'-arabino-uridine were synthesized and studied. The conjugates displayed significantly higher hybridization affinity to target DNA, increased fluorescence quantum yields of single-stranded oligonucleotides and their duplexes, and improved ability to form an interstrand excimer compared to analogous non-LNA probes.
Astakhova, I. V., Ustinov, A. V., Korshun, V. A., & Wengel, J. (2011). LNA for Optimization of Fluorescent Oligonucleotide Probes: Improved Spectral Properties and Target Binding. Bioconjugate Chemistry, 22(4), 533-9. https://doi.org/10.1021/bc1005027