LNA for Optimization of Fluorescent Oligonucleotide Probes: Improved Spectral Properties and Target Binding

Irina V Astakhova, Alexey V Ustinov, Vladimir A Korshun, Jesper Wengel

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Abstrakt

Mixmer LNA/DNA fluorescent probes containing the 1-(phenylethynyl)pyrene fluorophore attached to 2'-arabino-uridine were synthesized and studied. The conjugates displayed significantly higher hybridization affinity to target DNA, increased fluorescence quantum yields of single-stranded oligonucleotides and their duplexes, and improved ability to form an interstrand excimer compared to analogous non-LNA probes.
OriginalsprogEngelsk
TidsskriftBioconjugate Chemistry
Vol/bind22
Udgave nummer4
Sider (fra-til)533-9
Antal sider7
ISSN1043-1802
DOI
StatusUdgivet - 2011

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