Living whole-cell catalysis in compartmentalized emulsion

Qingcai Zhao, Marion B. Ansorge-Schumacher, Rainer Haag*, Changzhu Wu

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Resumé

Whole-cell biocatalysis plays an important role in biotransformation with unique features such as good tolerance of solvents and easy recycling. However, the relatively low catalytic efficiency limits their use in real production. In this study, a multi-compartmentalized emulsion in organic solvent was constructed to encapsulate living cells for enhanced catalytic performance. Extraordinary large interfacial area of the emulsion improved the bioactivity of Escherichia coli (E. Coli) cells up to 137 times compared to a standard biphasic system. The emulsion was stabilized by a biocompatible polymer and prepared by gentle shaking by hand, which resulted in good cell viability. Moreover, the encapsulated cells could be easily recycled, and the activity remained more than 70% after five cycles. This work provides a promising approach for utilizing whole-cell catalysts for efficient organic catalysis.

OriginalsprogEngelsk
Artikelnummer122221
TidsskriftBioresource Technology
Vol/bind295
Antal sider6
ISSN0960-8524
DOI
StatusUdgivet - jan. 2020

Fingeraftryk

catalysis
emulsion
Emulsions
Catalysis
Cells
bioactivity
biotransformation
Bioactivity
Organic solvents
Escherichia coli
Recycling
Polymers
viability
recycling
polymer
tolerance
catalyst
Catalysts

Citer dette

Zhao, Qingcai ; Ansorge-Schumacher, Marion B. ; Haag, Rainer ; Wu, Changzhu. / Living whole-cell catalysis in compartmentalized emulsion. I: Bioresource Technology. 2020 ; Bind 295.
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abstract = "Whole-cell biocatalysis plays an important role in biotransformation with unique features such as good tolerance of solvents and easy recycling. However, the relatively low catalytic efficiency limits their use in real production. In this study, a multi-compartmentalized emulsion in organic solvent was constructed to encapsulate living cells for enhanced catalytic performance. Extraordinary large interfacial area of the emulsion improved the bioactivity of Escherichia coli (E. Coli) cells up to 137 times compared to a standard biphasic system. The emulsion was stabilized by a biocompatible polymer and prepared by gentle shaking by hand, which resulted in good cell viability. Moreover, the encapsulated cells could be easily recycled, and the activity remained more than 70{\%} after five cycles. This work provides a promising approach for utilizing whole-cell catalysts for efficient organic catalysis.",
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author = "Qingcai Zhao and Ansorge-Schumacher, {Marion B.} and Rainer Haag and Changzhu Wu",
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Living whole-cell catalysis in compartmentalized emulsion. / Zhao, Qingcai; Ansorge-Schumacher, Marion B.; Haag, Rainer; Wu, Changzhu.

I: Bioresource Technology, Bind 295, 122221, 01.2020.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Living whole-cell catalysis in compartmentalized emulsion

AU - Zhao, Qingcai

AU - Ansorge-Schumacher, Marion B.

AU - Haag, Rainer

AU - Wu, Changzhu

PY - 2020/1

Y1 - 2020/1

N2 - Whole-cell biocatalysis plays an important role in biotransformation with unique features such as good tolerance of solvents and easy recycling. However, the relatively low catalytic efficiency limits their use in real production. In this study, a multi-compartmentalized emulsion in organic solvent was constructed to encapsulate living cells for enhanced catalytic performance. Extraordinary large interfacial area of the emulsion improved the bioactivity of Escherichia coli (E. Coli) cells up to 137 times compared to a standard biphasic system. The emulsion was stabilized by a biocompatible polymer and prepared by gentle shaking by hand, which resulted in good cell viability. Moreover, the encapsulated cells could be easily recycled, and the activity remained more than 70% after five cycles. This work provides a promising approach for utilizing whole-cell catalysts for efficient organic catalysis.

AB - Whole-cell biocatalysis plays an important role in biotransformation with unique features such as good tolerance of solvents and easy recycling. However, the relatively low catalytic efficiency limits their use in real production. In this study, a multi-compartmentalized emulsion in organic solvent was constructed to encapsulate living cells for enhanced catalytic performance. Extraordinary large interfacial area of the emulsion improved the bioactivity of Escherichia coli (E. Coli) cells up to 137 times compared to a standard biphasic system. The emulsion was stabilized by a biocompatible polymer and prepared by gentle shaking by hand, which resulted in good cell viability. Moreover, the encapsulated cells could be easily recycled, and the activity remained more than 70% after five cycles. This work provides a promising approach for utilizing whole-cell catalysts for efficient organic catalysis.

KW - Biocatalysis

KW - Block copolymer

KW - Compartmentalization

KW - Living-cell catalysis

KW - Multiple emulsion

U2 - 10.1016/j.biortech.2019.122221

DO - 10.1016/j.biortech.2019.122221

M3 - Journal article

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JO - Bioresource Technology

JF - Bioresource Technology

SN - 0960-8524

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