Lipid droplets interact with mitochondria using SNAP23

Sara Jägerström, Sam Polesie, Ylva Wickström, Bengt R Johansson, Henrik D Schröder, Kurt Højlund, Pontus Boström

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Udgivelsesdato: Sep
OriginalsprogEngelsk
TidsskriftCell Biology International
Vol/bind33
Udgave nummer9
Sider (fra-til)934-40
Antal sider6
ISSN1065-6995
DOI
StatusUdgivet - 1. sep. 2009

Fingeraftryk

SNARE Proteins
Peroxisomes
Confocal Microscopy
Small Interfering RNA
Lipid Droplets
Electron Microscopy
Fibroblasts
In Vitro Techniques

Citer dette

Jägerström, S., Polesie, S., Wickström, Y., Johansson, B. R., Schröder, H. D., Højlund, K., & Boström, P. (2009). Lipid droplets interact with mitochondria using SNAP23. Cell Biology International, 33(9), 934-40. https://doi.org/10.1016/j.cellbi.2009.06.011
Jägerström, Sara ; Polesie, Sam ; Wickström, Ylva ; Johansson, Bengt R ; Schröder, Henrik D ; Højlund, Kurt ; Boström, Pontus. / Lipid droplets interact with mitochondria using SNAP23. I: Cell Biology International. 2009 ; Bind 33, Nr. 9. s. 934-40.
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title = "Lipid droplets interact with mitochondria using SNAP23",
abstract = "Triglyceride-containing lipid droplets (LD) are dynamic organelles stored on demand in all cells. These droplets grow through a fusion process mediated by SNARE proteins, including SNAP23. The droplets have also been shown to be highly motile and interact with other cell organelles, including peroxisomes and the endoplasmic reticulum. We have used electron and confocal microscopy to demonstrate that LD form complexes with mitochondria in NIH 3T3 fibroblasts. Using an in vitro system of purified LD and mitochondria, we also show the formation of the LD-mitochondria complex, in which cytosolic factors are involved. Moreover, the presence of LD markers in mitochondria isolated by subcellular fractionations is demonstrated. Finally, ablation of SNAP23 using siRNA reduced complex formation and beta oxidation, which suggests that the LD-mitochondria complex is functional in the cell.",
author = "Sara J{\"a}gerstr{\"o}m and Sam Polesie and Ylva Wickstr{\"o}m and Johansson, {Bengt R} and Schr{\"o}der, {Henrik D} and Kurt H{\o}jlund and Pontus Bostr{\"o}m",
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Jägerström, S, Polesie, S, Wickström, Y, Johansson, BR, Schröder, HD, Højlund, K & Boström, P 2009, 'Lipid droplets interact with mitochondria using SNAP23', Cell Biology International, bind 33, nr. 9, s. 934-40. https://doi.org/10.1016/j.cellbi.2009.06.011

Lipid droplets interact with mitochondria using SNAP23. / Jägerström, Sara; Polesie, Sam; Wickström, Ylva; Johansson, Bengt R; Schröder, Henrik D; Højlund, Kurt; Boström, Pontus.

I: Cell Biology International, Bind 33, Nr. 9, 01.09.2009, s. 934-40.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Lipid droplets interact with mitochondria using SNAP23

AU - Jägerström, Sara

AU - Polesie, Sam

AU - Wickström, Ylva

AU - Johansson, Bengt R

AU - Schröder, Henrik D

AU - Højlund, Kurt

AU - Boström, Pontus

PY - 2009/9/1

Y1 - 2009/9/1

N2 - Triglyceride-containing lipid droplets (LD) are dynamic organelles stored on demand in all cells. These droplets grow through a fusion process mediated by SNARE proteins, including SNAP23. The droplets have also been shown to be highly motile and interact with other cell organelles, including peroxisomes and the endoplasmic reticulum. We have used electron and confocal microscopy to demonstrate that LD form complexes with mitochondria in NIH 3T3 fibroblasts. Using an in vitro system of purified LD and mitochondria, we also show the formation of the LD-mitochondria complex, in which cytosolic factors are involved. Moreover, the presence of LD markers in mitochondria isolated by subcellular fractionations is demonstrated. Finally, ablation of SNAP23 using siRNA reduced complex formation and beta oxidation, which suggests that the LD-mitochondria complex is functional in the cell.

AB - Triglyceride-containing lipid droplets (LD) are dynamic organelles stored on demand in all cells. These droplets grow through a fusion process mediated by SNARE proteins, including SNAP23. The droplets have also been shown to be highly motile and interact with other cell organelles, including peroxisomes and the endoplasmic reticulum. We have used electron and confocal microscopy to demonstrate that LD form complexes with mitochondria in NIH 3T3 fibroblasts. Using an in vitro system of purified LD and mitochondria, we also show the formation of the LD-mitochondria complex, in which cytosolic factors are involved. Moreover, the presence of LD markers in mitochondria isolated by subcellular fractionations is demonstrated. Finally, ablation of SNAP23 using siRNA reduced complex formation and beta oxidation, which suggests that the LD-mitochondria complex is functional in the cell.

U2 - 10.1016/j.cellbi.2009.06.011

DO - 10.1016/j.cellbi.2009.06.011

M3 - Journal article

VL - 33

SP - 934

EP - 940

JO - Cell Biology International

JF - Cell Biology International

SN - 1065-6995

IS - 9

ER -