LC-MS Analyses of Lipid Species in Skeletal Muscle Cells and Tissue

Marta Moreno-Torres, Jesper F Havelund, Nils J Faergeman

Publikation: Bidrag til bog/antologi/rapport/konference-proceedingBidrag til bog/antologiForskningpeer review

Resumé

Liquid chromatography-mass spectrometry (LC-MS) is a widely used methodology for measuring lipids at a global level. Combined with an optimal extraction method LC-MS enables the detection and characterization of a wide range of lipid species even of low abundance. Here, we describe two extraction- and LC-MS-based quantitative analytical methods for lipid, acyl-CoA, and acyl-carnitine analyses from either mouse C2C12 myotubes or mouse skeletal tissue. We also describe the use of 13C16-palmitate and its incorporation into acyl-carnitines to show how stable isotope tracers are metabolized within cells and therefore can be implemented for lipidomic flux analysis.

OriginalsprogEngelsk
TitelMyogenesis : Methods and protocols
RedaktørerSissel Beate Rønning
Udgivelses stedNew York
ForlagHumana Press
Publikationsdato2019
Sider213-228
Kapitel12
ISBN (Trykt)978-1-4939-8896-9
ISBN (Elektronisk)978-1-4939-8897-6
DOI
StatusUdgivet - 2019
NavnMethods in Molecular Biology
Nummer1889
ISSN1064-3745

Fingeraftryk

Liquid Chromatography
Skeletal Muscle
Lipids
Muscles
Palmitates
Isotopes

Citer dette

Moreno-Torres, M., Havelund, J. F., & Faergeman, N. J. (2019). LC-MS Analyses of Lipid Species in Skeletal Muscle Cells and Tissue. I S. B. Rønning (red.), Myogenesis: Methods and protocols (s. 213-228). New York: Humana Press. Methods in Molecular Biology, Nr. 1889 https://doi.org/10.1007/978-1-4939-8897-6_12
Moreno-Torres, Marta ; Havelund, Jesper F ; Faergeman, Nils J. / LC-MS Analyses of Lipid Species in Skeletal Muscle Cells and Tissue. Myogenesis: Methods and protocols. red. / Sissel Beate Rønning. New York : Humana Press, 2019. s. 213-228 (Methods in Molecular Biology; Nr. 1889).
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Moreno-Torres, M, Havelund, JF & Faergeman, NJ 2019, LC-MS Analyses of Lipid Species in Skeletal Muscle Cells and Tissue. i SB Rønning (red.), Myogenesis: Methods and protocols. Humana Press, New York, Methods in Molecular Biology, nr. 1889, s. 213-228. https://doi.org/10.1007/978-1-4939-8897-6_12

LC-MS Analyses of Lipid Species in Skeletal Muscle Cells and Tissue. / Moreno-Torres, Marta; Havelund, Jesper F; Faergeman, Nils J.

Myogenesis: Methods and protocols. red. / Sissel Beate Rønning. New York : Humana Press, 2019. s. 213-228 (Methods in Molecular Biology; Nr. 1889).

Publikation: Bidrag til bog/antologi/rapport/konference-proceedingBidrag til bog/antologiForskningpeer review

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N2 - Liquid chromatography-mass spectrometry (LC-MS) is a widely used methodology for measuring lipids at a global level. Combined with an optimal extraction method LC-MS enables the detection and characterization of a wide range of lipid species even of low abundance. Here, we describe two extraction- and LC-MS-based quantitative analytical methods for lipid, acyl-CoA, and acyl-carnitine analyses from either mouse C2C12 myotubes or mouse skeletal tissue. We also describe the use of 13C16-palmitate and its incorporation into acyl-carnitines to show how stable isotope tracers are metabolized within cells and therefore can be implemented for lipidomic flux analysis.

AB - Liquid chromatography-mass spectrometry (LC-MS) is a widely used methodology for measuring lipids at a global level. Combined with an optimal extraction method LC-MS enables the detection and characterization of a wide range of lipid species even of low abundance. Here, we describe two extraction- and LC-MS-based quantitative analytical methods for lipid, acyl-CoA, and acyl-carnitine analyses from either mouse C2C12 myotubes or mouse skeletal tissue. We also describe the use of 13C16-palmitate and its incorporation into acyl-carnitines to show how stable isotope tracers are metabolized within cells and therefore can be implemented for lipidomic flux analysis.

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Moreno-Torres M, Havelund JF, Faergeman NJ. LC-MS Analyses of Lipid Species in Skeletal Muscle Cells and Tissue. I Rønning SB, red., Myogenesis: Methods and protocols. New York: Humana Press. 2019. s. 213-228. (Methods in Molecular Biology; Nr. 1889). https://doi.org/10.1007/978-1-4939-8897-6_12