Label-free observation of DNA-encoded liposome fusion by surface plasmon resonance

Philipp M.G. Löffler; Nikolaj A. Risgaard; Bettina L. Svendsen; Katrine A. Jepsen; Alexander Rabe; Stefan Vogel

doi:10.1039/d3cc02793g

Label-free observation of DNA-encoded liposome fusion by surface plasmon resonance

Philipp M.G. Löffler, Nikolaj A. Risgaard, Bettina L. Svendsen, Katrine A. Jepsen, Alexander Rabe, Stefan Vogel^*

^*Kontaktforfatter

Syddansk Universitet

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

Abstract

Assembly and fusion between different populations of lipid nanoparticles was mediated by membrane-anchored lipidated nucleic acid (LiNA) strands and observed using surface plasmon resonance (SPR) as a label-free real-time assay. Irreversible membrane fusion was distinguished from reversible assembly by enzymatical cleavage of dsDNA tethers in situ. The assay enables user-friendly monitoring and application of membrane fusion in the context of liposomal drug delivery or synthetic biology.

Originalsprog	Engelsk
Tidsskrift	Chemical Communications
Vol/bind	59
Udgave nummer	70
Sider (fra-til)	10548-10551
ISSN	1359-7345
DOI	https://doi.org/10.1039/d3cc02793g
Status	Udgivet - 2023

Bibliografisk note

Publisher Copyright:
© 2023 The Royal Society of Chemistry.

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10.1039/d3cc02793g

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1 Ph.d.-afhandling

Programmed Chemistry in Lipid-Nanocompartments
Risgaard, N., 9. sep. 2022, Syddansk Universitet. Det Naturvidenskabelige Fakultet. 333 s.
Publikation: Afhandling › Ph.d.-afhandling

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abstract = "Assembly and fusion between different populations of lipid nanoparticles was mediated by membrane-anchored lipidated nucleic acid (LiNA) strands and observed using surface plasmon resonance (SPR) as a label-free real-time assay. Irreversible membrane fusion was distinguished from reversible assembly by enzymatical cleavage of dsDNA tethers in situ. The assay enables user-friendly monitoring and application of membrane fusion in the context of liposomal drug delivery or synthetic biology.",

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AU - Löffler, Philipp M.G.

AU - Risgaard, Nikolaj A.

AU - Svendsen, Bettina L.

AU - Jepsen, Katrine A.

AU - Rabe, Alexander

AU - Vogel, Stefan

PY - 2023

Y1 - 2023

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AB - Assembly and fusion between different populations of lipid nanoparticles was mediated by membrane-anchored lipidated nucleic acid (LiNA) strands and observed using surface plasmon resonance (SPR) as a label-free real-time assay. Irreversible membrane fusion was distinguished from reversible assembly by enzymatical cleavage of dsDNA tethers in situ. The assay enables user-friendly monitoring and application of membrane fusion in the context of liposomal drug delivery or synthetic biology.

U2 - 10.1039/d3cc02793g

DO - 10.1039/d3cc02793g

M3 - Journal article

C2 - 37566388

AN - SCOPUS:85168566080

SN - 1359-7345

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SP - 10548

EP - 10551

JO - Chemical Communications

JF - Chemical Communications

IS - 70

ER -

Label-free observation of DNA-encoded liposome fusion by surface plasmon resonance

Abstract

Bibliografisk note

Dokumenter og links

SDU link

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Programmed Chemistry in Lipid-Nanocompartments

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