Label free noninvasive spatially resolved NaCl concentration measurements using Coherent Anti-Stokes Raman Scattering microscopy applied to butter

Brian B. Jensen, Zachary J. Glover, Simon M.M. Pedersen, Ulf Andersen, Lars Duelund, Jonathan R. Brewer

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Imaging the microstructure of opaque composite foodstuffs and extracting quantitative chemical information about specific localized components is challenging. Herein, a method has been developed to determine spatially resolved concentrations of aqueous salt and applied to measure salt concentrations of water droplets in butter samples. This was done using Coherent Anti-Stokes Raman Scattering (CARS) microscopy which achieves non-invasive label free imaging based on visualization of specific chemical-bond vibrations. The concentration of salt in the dispersed water droplets in butter was determined based on the relative change in intensity of the CARS-signal at two distinct wavenumbers, which have been shown to be dependent on the inter-molecular coupling of water molecules and salt. The results provide the size and salt concentration distribution of the droplets in the samples. It is further shown that the average salt concentration in the whole sample can correctly be inferred from the concentration measured within the water droplets.

OriginalsprogEngelsk
Artikelnummer124881
TidsskriftFood Chemistry
Vol/bind297
Antal sider6
ISSN0308-8146
DOI
StatusUdgivet - 1. nov. 2019

Fingeraftryk

Raman spectroscopy
butter
Raman scattering
Labels
Microscopy
microscopy
Microscopic examination
salt concentration
Salts
droplets
Water
water
image analysis
chemical bonding
salts
Imaging techniques
vibration
sampling
Chemical bonds
microstructure

Citer dette

@article{515de140d1a34f9e865a49c084e8f7e5,
title = "Label free noninvasive spatially resolved NaCl concentration measurements using Coherent Anti-Stokes Raman Scattering microscopy applied to butter",
abstract = "Imaging the microstructure of opaque composite foodstuffs and extracting quantitative chemical information about specific localized components is challenging. Herein, a method has been developed to determine spatially resolved concentrations of aqueous salt and applied to measure salt concentrations of water droplets in butter samples. This was done using Coherent Anti-Stokes Raman Scattering (CARS) microscopy which achieves non-invasive label free imaging based on visualization of specific chemical-bond vibrations. The concentration of salt in the dispersed water droplets in butter was determined based on the relative change in intensity of the CARS-signal at two distinct wavenumbers, which have been shown to be dependent on the inter-molecular coupling of water molecules and salt. The results provide the size and salt concentration distribution of the droplets in the samples. It is further shown that the average salt concentration in the whole sample can correctly be inferred from the concentration measured within the water droplets.",
keywords = "Butter, CARS, Coherent Anti-Stokes Raman Scattering, Dairy, Oil-in-water emulsion, Raman, Salt",
author = "Jensen, {Brian B.} and Glover, {Zachary J.} and Pedersen, {Simon M.M.} and Ulf Andersen and Lars Duelund and Brewer, {Jonathan R.}",
year = "2019",
month = "11",
day = "1",
doi = "10.1016/j.foodchem.2019.05.155",
language = "English",
volume = "297",
journal = "Food Chemistry",
issn = "0308-8146",
publisher = "Elsevier",

}

Label free noninvasive spatially resolved NaCl concentration measurements using Coherent Anti-Stokes Raman Scattering microscopy applied to butter. / Jensen, Brian B.; Glover, Zachary J.; Pedersen, Simon M.M.; Andersen, Ulf; Duelund, Lars; Brewer, Jonathan R.

I: Food Chemistry, Bind 297, 124881, 01.11.2019.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Label free noninvasive spatially resolved NaCl concentration measurements using Coherent Anti-Stokes Raman Scattering microscopy applied to butter

AU - Jensen, Brian B.

AU - Glover, Zachary J.

AU - Pedersen, Simon M.M.

AU - Andersen, Ulf

AU - Duelund, Lars

AU - Brewer, Jonathan R.

PY - 2019/11/1

Y1 - 2019/11/1

N2 - Imaging the microstructure of opaque composite foodstuffs and extracting quantitative chemical information about specific localized components is challenging. Herein, a method has been developed to determine spatially resolved concentrations of aqueous salt and applied to measure salt concentrations of water droplets in butter samples. This was done using Coherent Anti-Stokes Raman Scattering (CARS) microscopy which achieves non-invasive label free imaging based on visualization of specific chemical-bond vibrations. The concentration of salt in the dispersed water droplets in butter was determined based on the relative change in intensity of the CARS-signal at two distinct wavenumbers, which have been shown to be dependent on the inter-molecular coupling of water molecules and salt. The results provide the size and salt concentration distribution of the droplets in the samples. It is further shown that the average salt concentration in the whole sample can correctly be inferred from the concentration measured within the water droplets.

AB - Imaging the microstructure of opaque composite foodstuffs and extracting quantitative chemical information about specific localized components is challenging. Herein, a method has been developed to determine spatially resolved concentrations of aqueous salt and applied to measure salt concentrations of water droplets in butter samples. This was done using Coherent Anti-Stokes Raman Scattering (CARS) microscopy which achieves non-invasive label free imaging based on visualization of specific chemical-bond vibrations. The concentration of salt in the dispersed water droplets in butter was determined based on the relative change in intensity of the CARS-signal at two distinct wavenumbers, which have been shown to be dependent on the inter-molecular coupling of water molecules and salt. The results provide the size and salt concentration distribution of the droplets in the samples. It is further shown that the average salt concentration in the whole sample can correctly be inferred from the concentration measured within the water droplets.

KW - Butter

KW - CARS

KW - Coherent Anti-Stokes Raman Scattering

KW - Dairy

KW - Oil-in-water emulsion

KW - Raman

KW - Salt

U2 - 10.1016/j.foodchem.2019.05.155

DO - 10.1016/j.foodchem.2019.05.155

M3 - Journal article

VL - 297

JO - Food Chemistry

JF - Food Chemistry

SN - 0308-8146

M1 - 124881

ER -