Kinetics of phycocyanobilin cleavage from C-phycocyanin by methanolysis

Chandrakant Ramkrishna Malwade; Maria Cinta Roda Serrat; Knud Villy Christensen; Xavier Fretté; Lars Porskjær  Christensen

doi:10.1016/B978-0-444-63428-3.50015-1

Kinetics of phycocyanobilin cleavage from C-phycocyanin by methanolysis

Chandrakant Ramkrishna Malwade, Maria Cinta Roda Serrat, Knud Villy Christensen, Xavier Fretté, Lars Porskjær Christensen

Institut for Grøn Teknologi

Publikation: Kapitel i bog/rapport/konference-proceeding › Konferencebidrag i proceedings › Forskning › peer review

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Abstract

Phycocyanobilin (PCB) is an important linear tetrapyrrolic molecule for food as well as pharmaceutical industry. It is obtained from blue-green algae, where it is attached covalently to phycobiliproteins (C-PC and APC) present in the light harvesting complexes. In this work, cleavage of PCB from phycobiliproteins present in the extract of Arthrospira platensis by methanolysis is investigated. Different initial concentrations (25 mg/mL, 10 mg/mL, and 5 mg/mL) of proteins are used in order to investigate the effect of protein aggregation on process yield. A kinetic model is developed by fitting the experimental data for methanolysis. Results show that the kinetics follows a pseudo first order kinetics and remains unaffected due to the different initial concentration of
phycobiliproteins. Moreover, yield of PCB in the cleavage process is found to be
proportional to the initial concentration of phycobiliproteins.

Originalsprog	Engelsk
Titel	Computer - Aided Chemical Engineering
Redaktører	Zdravko Kravanja, Miloš Bogataj
Udgivelsessted	Amsterdam
Forlag	Elsevier Masson
Publikationsdato	12. jun. 2016
Sider	61-66
ISBN (Trykt)	978-0-444-63428-3
ISBN (Elektronisk)	978-0-444-63873-1
DOI	https://doi.org/10.1016/B978-0-444-63428-3.50015-1
Status	Udgivet - 12. jun. 2016
Begivenhed	26th European Symposium on Computer Aided Process Engineering - Portoroz, Slovenien Varighed: 12. jun. 2016 → 15. jun. 2016 Konferencens nummer: 26

Konference

Konference	26th European Symposium on Computer Aided Process Engineering
Nummer	26
Land/Område	Slovenien
By	Portoroz
Periode	12/06/2016 → 15/06/2016

Navn	Computer Aided Chemical Engineering
Vol/bind	38
ISSN	1570-7946

Dokumenter og links

10.1016/B978-0-444-63428-3.50015-1

ESCAPE 26_Kinetics of Phycocyanobilin CleavageAnden version, 567 KB

26th European Symposium on Computer Aided Process Engineering
Malwade, C. R. (Deltager)
12. jun. 2016 → 15. jun. 2016
Aktivitet: Deltagelse i faglig begivenhed › Organisering af eller deltagelse i konference

Citationsformater

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title = "Kinetics of phycocyanobilin cleavage from C-phycocyanin by methanolysis",

abstract = "Phycocyanobilin (PCB) is an important linear tetrapyrrolic molecule for food as well as pharmaceutical industry. It is obtained from blue-green algae, where it is attached covalently to phycobiliproteins (C-PC and APC) present in the light harvesting complexes. In this work, cleavage of PCB from phycobiliproteins present in the extract of Arthrospira platensis by methanolysis is investigated. Different initial concentrations (25 mg/mL, 10 mg/mL, and 5 mg/mL) of proteins are used in order to investigate the effect of protein aggregation on process yield. A kinetic model is developed by fitting the experimental data for methanolysis. Results show that the kinetics follows a pseudo first order kinetics and remains unaffected due to the different initial concentration ofphycobiliproteins. Moreover, yield of PCB in the cleavage process is found to beproportional to the initial concentration of phycobiliproteins.",

keywords = "Methanolysis, reaction kinetics, food color, phycocyanobilin, Arthrospira platensis",

author = "Malwade, {Chandrakant Ramkrishna} and {Roda Serrat}, {Maria Cinta} and Christensen, {Knud Villy} and Xavier Frett{\'e} and Christensen, {Lars Porskj{\ae}r}",

year = "2016",

month = jun,

day = "12",

doi = "10.1016/B978-0-444-63428-3.50015-1",

language = "English",

isbn = "978-0-444-63428-3",

series = "Computer Aided Chemical Engineering",

publisher = "Elsevier Masson",

pages = "61--66",

editor = "{ Kravanja}, Zdravko and Milo{\v s} Bogataj",

booktitle = "Computer - Aided Chemical Engineering",

address = "France",

note = "26th European Symposium on Computer Aided Process Engineering, ESCAPE ; Conference date: 12-06-2016 Through 15-06-2016",

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Malwade, CR, Roda Serrat, MC, Christensen, KV , Fretté, X & Christensen, LP 2016, Kinetics of phycocyanobilin cleavage from C-phycocyanin by methanolysis. i Z Kravanja & M Bogataj (red), Computer - Aided Chemical Engineering. Elsevier Masson, Amsterdam, Computer Aided Chemical Engineering, bind 38, s. 61-66, 26th European Symposium on Computer Aided Process Engineering, Portoroz, Slovenien, 12/06/2016. https://doi.org/10.1016/B978-0-444-63428-3.50015-1

Kinetics of phycocyanobilin cleavage from C-phycocyanin by methanolysis. / Malwade, Chandrakant Ramkrishna; Roda Serrat, Maria Cinta; Christensen, Knud Villy et al.
Computer - Aided Chemical Engineering. red. / Zdravko Kravanja; Miloš Bogataj. Amsterdam: Elsevier Masson, 2016. s. 61-66 (Computer Aided Chemical Engineering, Bind 38).

Publikation: Kapitel i bog/rapport/konference-proceeding › Konferencebidrag i proceedings › Forskning › peer review

TY - GEN

T1 - Kinetics of phycocyanobilin cleavage from C-phycocyanin by methanolysis

AU - Malwade, Chandrakant Ramkrishna

AU - Roda Serrat, Maria Cinta

AU - Christensen, Knud Villy

AU - Fretté, Xavier

AU - Christensen, Lars Porskjær

N1 - Conference code: 26

PY - 2016/6/12

Y1 - 2016/6/12

N2 - Phycocyanobilin (PCB) is an important linear tetrapyrrolic molecule for food as well as pharmaceutical industry. It is obtained from blue-green algae, where it is attached covalently to phycobiliproteins (C-PC and APC) present in the light harvesting complexes. In this work, cleavage of PCB from phycobiliproteins present in the extract of Arthrospira platensis by methanolysis is investigated. Different initial concentrations (25 mg/mL, 10 mg/mL, and 5 mg/mL) of proteins are used in order to investigate the effect of protein aggregation on process yield. A kinetic model is developed by fitting the experimental data for methanolysis. Results show that the kinetics follows a pseudo first order kinetics and remains unaffected due to the different initial concentration ofphycobiliproteins. Moreover, yield of PCB in the cleavage process is found to beproportional to the initial concentration of phycobiliproteins.

AB - Phycocyanobilin (PCB) is an important linear tetrapyrrolic molecule for food as well as pharmaceutical industry. It is obtained from blue-green algae, where it is attached covalently to phycobiliproteins (C-PC and APC) present in the light harvesting complexes. In this work, cleavage of PCB from phycobiliproteins present in the extract of Arthrospira platensis by methanolysis is investigated. Different initial concentrations (25 mg/mL, 10 mg/mL, and 5 mg/mL) of proteins are used in order to investigate the effect of protein aggregation on process yield. A kinetic model is developed by fitting the experimental data for methanolysis. Results show that the kinetics follows a pseudo first order kinetics and remains unaffected due to the different initial concentration ofphycobiliproteins. Moreover, yield of PCB in the cleavage process is found to beproportional to the initial concentration of phycobiliproteins.

KW - Methanolysis

KW - reaction kinetics

KW - food color

KW - phycocyanobilin

KW - Arthrospira platensis

U2 - 10.1016/B978-0-444-63428-3.50015-1

DO - 10.1016/B978-0-444-63428-3.50015-1

M3 - Article in proceedings

SN - 978-0-444-63428-3

T3 - Computer Aided Chemical Engineering

SP - 61

EP - 66

BT - Computer - Aided Chemical Engineering

A2 - Kravanja, Zdravko

A2 - Bogataj, Miloš

PB - Elsevier Masson

CY - Amsterdam

T2 - 26th European Symposium on Computer Aided Process Engineering

Y2 - 12 June 2016 through 15 June 2016

ER -

Kinetics of phycocyanobilin cleavage from C-phycocyanin by methanolysis

Abstract

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26th European Symposium on Computer Aided Process Engineering

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