Isolation and characterization of a monoclonal anti-protein kinase CK2 beta-subunit antibody of the IgG class for the direct detection of CK2 beta-subunit in tissue cultures of various mammalian species and human tumors

W Nastainczyk, I Schmidt-Spaniol, B Boldyreff, O G Issinger

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Udgivelsesdato: 1995-Aug
OriginalsprogEngelsk
TidsskriftHybridoma
Vol/bind14
Udgave nummer4
Sider (fra-til)335-339
Antal sider4
ISSN0272-457X
StatusUdgivet - 1. aug. 1995

Fingeraftryk

Casein Kinase II
Neoplasms
Holoenzymes
Complex Mixtures
Immunoblotting
Cricetinae

Citer dette

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title = "Isolation and characterization of a monoclonal anti-protein kinase CK2 beta-subunit antibody of the IgG class for the direct detection of CK2 beta-subunit in tissue cultures of various mammalian species and human tumors",
abstract = "A murine monoclonal anti-protein kinase CK2 beta antibody was isolated and characterized. The antibody detects 1 pmol of purified recombinant CK2 beta-subunit after analysis on SDS-PAGE. Alternatively undenatured CK2 beta-subunit was detected by an ELISA assay either as recombinant CK2 beta-subunit or in the CK2 holoenzyme (alpha 2 beta 2). Here, concentrations of the first antibody of 1 ng/ml still allowed the detection of the subunit. Immunoblotting of crude cellular extracts from various tissue cultures (man, mouse, and hamster), from human tumors, and the nonneoplastic tissue allowed the detection of the CK2 beta-subunit. The detected epitope of this antibody was, as determined by the epitope analysis technique, 123GLSDI127.",
keywords = "Amino Acid Sequence, Animals, Antibodies, Monoclonal, Casein Kinase II, Cricetinae, Culture Techniques, Epitope Mapping, Humans, Immunoglobulin G, Mice, Mice, Inbred BALB C, Molecular Sequence Data, Protein-Serine-Threonine Kinases, Tumor Cells, Cultured",
author = "W Nastainczyk and I Schmidt-Spaniol and B Boldyreff and Issinger, {O G}",
year = "1995",
month = "8",
day = "1",
language = "English",
volume = "14",
pages = "335--339",
journal = "Hybridoma",
issn = "1554-0014",
publisher = "Mary Ann Liebert Incorporated",
number = "4",

}

Isolation and characterization of a monoclonal anti-protein kinase CK2 beta-subunit antibody of the IgG class for the direct detection of CK2 beta-subunit in tissue cultures of various mammalian species and human tumors. / Nastainczyk, W; Schmidt-Spaniol, I; Boldyreff, B; Issinger, O G.

I: Hybridoma, Bind 14, Nr. 4, 01.08.1995, s. 335-339.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Isolation and characterization of a monoclonal anti-protein kinase CK2 beta-subunit antibody of the IgG class for the direct detection of CK2 beta-subunit in tissue cultures of various mammalian species and human tumors

AU - Nastainczyk, W

AU - Schmidt-Spaniol, I

AU - Boldyreff, B

AU - Issinger, O G

PY - 1995/8/1

Y1 - 1995/8/1

N2 - A murine monoclonal anti-protein kinase CK2 beta antibody was isolated and characterized. The antibody detects 1 pmol of purified recombinant CK2 beta-subunit after analysis on SDS-PAGE. Alternatively undenatured CK2 beta-subunit was detected by an ELISA assay either as recombinant CK2 beta-subunit or in the CK2 holoenzyme (alpha 2 beta 2). Here, concentrations of the first antibody of 1 ng/ml still allowed the detection of the subunit. Immunoblotting of crude cellular extracts from various tissue cultures (man, mouse, and hamster), from human tumors, and the nonneoplastic tissue allowed the detection of the CK2 beta-subunit. The detected epitope of this antibody was, as determined by the epitope analysis technique, 123GLSDI127.

AB - A murine monoclonal anti-protein kinase CK2 beta antibody was isolated and characterized. The antibody detects 1 pmol of purified recombinant CK2 beta-subunit after analysis on SDS-PAGE. Alternatively undenatured CK2 beta-subunit was detected by an ELISA assay either as recombinant CK2 beta-subunit or in the CK2 holoenzyme (alpha 2 beta 2). Here, concentrations of the first antibody of 1 ng/ml still allowed the detection of the subunit. Immunoblotting of crude cellular extracts from various tissue cultures (man, mouse, and hamster), from human tumors, and the nonneoplastic tissue allowed the detection of the CK2 beta-subunit. The detected epitope of this antibody was, as determined by the epitope analysis technique, 123GLSDI127.

KW - Amino Acid Sequence

KW - Animals

KW - Antibodies, Monoclonal

KW - Casein Kinase II

KW - Cricetinae

KW - Culture Techniques

KW - Epitope Mapping

KW - Humans

KW - Immunoglobulin G

KW - Mice

KW - Mice, Inbred BALB C

KW - Molecular Sequence Data

KW - Protein-Serine-Threonine Kinases

KW - Tumor Cells, Cultured

M3 - Journal article

C2 - 8522344

VL - 14

SP - 335

EP - 339

JO - Hybridoma

JF - Hybridoma

SN - 1554-0014

IS - 4

ER -