Investigation of the Use of Ceramic Membranes in Recovering Liquid Enzymes for Castor Oil Transesterification

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Resumé

The reuse of the liquid enzymes Eversa Transform and Resinase HT for castor oil transesterification was investigated. For each enzyme, the reactions were carried out at 35 °C with 6:1 methanol-to-oil molar ratio, 5 wt% enzyme and 5 wt% water by weight of oil, for 8 hours. After completion, the reaction mixture was centrifuged at 4000 rpm for 30 min and the enzyme phase was collected. The reuse of the enzyme solutions were tested using three different separation techniques. In the first, enzymes were reused after the centrifugation step without further separation. In the second, enzymes were reused after centrifugation followed by water diafiltration using an ultrafiltration membrane in order to remove glycerol and methanol from the recycle solution. In the third, the enzyme solution obtained after diafiltration was concentrated in the membrane set-up prior to its reuse. Tubular ceramic ultrafiltration membranes with MWCO of 15 and 25 kDa were used.
The experiments showed that ultrafiltration removed glycerol and methanol from the enzyme solution, increasing the enzyme activity during the transesterification. Furthermore, concentration of the enzyme solutions after the diafiltration had a positive effect on the FAME production when recycled enzyme solutions are used. For Eversa Transform, the biodiesel content increased from 67 % with reuse after centrifugation to 83 % when a concentrated and diafiltrated enzyme solution was used as catalyst. For Resinase HT, the biodiesel concentration improved from 69 to up to 79 %.
OriginalsprogEngelsk
BogserieChemical engineering transactions
Vol/bind74
Sider (fra-til)769-774
ISSN1974-9791
DOI
StatusUdgivet - 31. maj 2019
Begivenhed14th International Conference on Chemical and Process Engineering - RoyalCarlton Hotel, Via Montebello 8, Bologna, Italien
Varighed: 26. maj 201929. maj 2019
Konferencens nummer: 14
http://www.aidic.it/icheap14/

Konference

Konference14th International Conference on Chemical and Process Engineering
Nummer14
LokationRoyalCarlton Hotel, Via Montebello 8
LandItalien
ByBologna
Periode26/05/201929/05/2019
Internetadresse

Fingeraftryk

Castor Oil
Ceramic membranes
Transesterification
Enzymes
Liquids
Centrifugation
Ultrafiltration
Methanol
Biofuels
Biodiesel
Membranes
Glycerol
Oils
Mathematical transformations
Water
Enzyme activity

Citer dette

@inproceedings{724082871c8c418b8c1c4a17096eba23,
title = "Investigation of the Use of Ceramic Membranes in Recovering Liquid Enzymes for Castor Oil Transesterification",
abstract = "The reuse of the liquid enzymes Eversa Transform and Resinase HT for castor oil transesterification was investigated. For each enzyme, the reactions were carried out at 35 °C with 6:1 methanol-to-oil molar ratio, 5 wt{\%} enzyme and 5 wt{\%} water by weight of oil, for 8 hours. After completion, the reaction mixture was centrifuged at 4000 rpm for 30 min and the enzyme phase was collected. The reuse of the enzyme solutions were tested using three different separation techniques. In the first, enzymes were reused after the centrifugation step without further separation. In the second, enzymes were reused after centrifugation followed by water diafiltration using an ultrafiltration membrane in order to remove glycerol and methanol from the recycle solution. In the third, the enzyme solution obtained after diafiltration was concentrated in the membrane set-up prior to its reuse. Tubular ceramic ultrafiltration membranes with MWCO of 15 and 25 kDa were used. The experiments showed that ultrafiltration removed glycerol and methanol from the enzyme solution, increasing the enzyme activity during the transesterification. Furthermore, concentration of the enzyme solutions after the diafiltration had a positive effect on the FAME production when recycled enzyme solutions are used. For Eversa Transform, the biodiesel content increased from 67 {\%} with reuse after centrifugation to 83 {\%} when a concentrated and diafiltrated enzyme solution was used as catalyst. For Resinase HT, the biodiesel concentration improved from 69 to up to 79 {\%}.",
author = "Andrade, {Thalles Allan} and Massimiliano Errico and Christensen, {Knud Villy}",
year = "2019",
month = "5",
day = "31",
doi = "10.3303/CET1974129",
language = "English",
volume = "74",
pages = "769--774",
journal = "Chemical engineering transactions",
issn = "1974-9791",
publisher = "AIDIC",

}

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T1 - Investigation of the Use of Ceramic Membranes in Recovering Liquid Enzymes for Castor Oil Transesterification

AU - Andrade, Thalles Allan

AU - Errico, Massimiliano

AU - Christensen, Knud Villy

PY - 2019/5/31

Y1 - 2019/5/31

N2 - The reuse of the liquid enzymes Eversa Transform and Resinase HT for castor oil transesterification was investigated. For each enzyme, the reactions were carried out at 35 °C with 6:1 methanol-to-oil molar ratio, 5 wt% enzyme and 5 wt% water by weight of oil, for 8 hours. After completion, the reaction mixture was centrifuged at 4000 rpm for 30 min and the enzyme phase was collected. The reuse of the enzyme solutions were tested using three different separation techniques. In the first, enzymes were reused after the centrifugation step without further separation. In the second, enzymes were reused after centrifugation followed by water diafiltration using an ultrafiltration membrane in order to remove glycerol and methanol from the recycle solution. In the third, the enzyme solution obtained after diafiltration was concentrated in the membrane set-up prior to its reuse. Tubular ceramic ultrafiltration membranes with MWCO of 15 and 25 kDa were used. The experiments showed that ultrafiltration removed glycerol and methanol from the enzyme solution, increasing the enzyme activity during the transesterification. Furthermore, concentration of the enzyme solutions after the diafiltration had a positive effect on the FAME production when recycled enzyme solutions are used. For Eversa Transform, the biodiesel content increased from 67 % with reuse after centrifugation to 83 % when a concentrated and diafiltrated enzyme solution was used as catalyst. For Resinase HT, the biodiesel concentration improved from 69 to up to 79 %.

AB - The reuse of the liquid enzymes Eversa Transform and Resinase HT for castor oil transesterification was investigated. For each enzyme, the reactions were carried out at 35 °C with 6:1 methanol-to-oil molar ratio, 5 wt% enzyme and 5 wt% water by weight of oil, for 8 hours. After completion, the reaction mixture was centrifuged at 4000 rpm for 30 min and the enzyme phase was collected. The reuse of the enzyme solutions were tested using three different separation techniques. In the first, enzymes were reused after the centrifugation step without further separation. In the second, enzymes were reused after centrifugation followed by water diafiltration using an ultrafiltration membrane in order to remove glycerol and methanol from the recycle solution. In the third, the enzyme solution obtained after diafiltration was concentrated in the membrane set-up prior to its reuse. Tubular ceramic ultrafiltration membranes with MWCO of 15 and 25 kDa were used. The experiments showed that ultrafiltration removed glycerol and methanol from the enzyme solution, increasing the enzyme activity during the transesterification. Furthermore, concentration of the enzyme solutions after the diafiltration had a positive effect on the FAME production when recycled enzyme solutions are used. For Eversa Transform, the biodiesel content increased from 67 % with reuse after centrifugation to 83 % when a concentrated and diafiltrated enzyme solution was used as catalyst. For Resinase HT, the biodiesel concentration improved from 69 to up to 79 %.

U2 - 10.3303/CET1974129

DO - 10.3303/CET1974129

M3 - Conference article

VL - 74

SP - 769

EP - 774

JO - Chemical engineering transactions

JF - Chemical engineering transactions

SN - 1974-9791

ER -