Interpretation of Tandem Mass Spectrometry (MSMS) Spectra for Peptide Analysis

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Abstrakt

The aim of this chapter is to give a short introduction to peptide analysis by mass spectrometry (MS) and interpretation of fragment mass spectra. Through examples and guidelines we demonstrate how to understand and validate search results and how to perform de novo sequencing based on the often very complex fragmentation pattern obtained by tandem mass spectrometry (also referred to as MSMS). The focus is on simple rules for interpretation of MSMS spectra of tryptic as well as non-tryptic peptides.

OriginalsprogEngelsk
TitelPeptide antibodies : methods and protocols
Vol/bind1348
ForlagHumana Press
Publikationsdato2015
Sider83-102
ISBN (Trykt)978-1-4939-2998-6
DOI
StatusUdgivet - 2015
NavnMethods in Molecular Biology
Vol/bind1348
ISSN1064-3745

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Citationsformater

Hjernø, K., & Højrup, P. (2015). Interpretation of Tandem Mass Spectrometry (MSMS) Spectra for Peptide Analysis. I Peptide antibodies: methods and protocols (Bind 1348, s. 83-102). Humana Press. Methods in Molecular Biology, Bind. 1348 https://doi.org/10.1007/978-1-4939-2999-3_10