Interlaboratory study of the IFCC method for alanine aminotransferase performed with use of a partly purified reference material

F. Schiele*, J. Muller, E. Colinet, G. Siest, P. Arzoglou, H. Brettschneider, D. H. Calam, F. Ceriotti, G. Ferard, J. Frei, A. Hadjivassiliou, J. C.M. Hafkenscheid, M. Horder, F. J. Gella, R. Rej, E. Schmidt, A. Vassault, V. Viette

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Abstrakt

We present the results of a study on performance of a reference material for alanine aminotransferase (ALT, EC 2.6.1.2) and the corresponding IFCC- approved method in an interlaboratory trial involving 13 laboratories. The ALT material was partly purified from pig heart (specific activity, 150 kU/g) and was essentially free of six potentially contaminating enzyme activities, including aspartate aminotransferase (EC 2.6.1.1). The partly purified ALT was lyophilized in a triethanolamine-buffered matrix, pH 6.4, containing bovine serum albumin and saccharose. Under these conditions, the predicted yearly loss of activity was 0.02% at 4 °C and <0.01% at -20 °C. The final blank-corrected results of the accepted set of data gave a mean (SD) of 128.5 (5.1) U/L. The among-laboratory SD was 4.6 U/L and the within-laboratory SD was 2.0 U/L. The certified ALT catalytic concentration in the reconstituted material was 129 U/L with a 0.95 confidence interval of ± 4 U/L.

OriginalsprogEngelsk
TidsskriftClinical Chemistry
Vol/bind38
Udgave nummer12
Sider (fra-til)2365-2371
Antal sider7
ISSN0009-9147
StatusUdgivet - 1. dec. 1992

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  • Citationsformater

    Schiele, F., Muller, J., Colinet, E., Siest, G., Arzoglou, P., Brettschneider, H., Calam, D. H., Ceriotti, F., Ferard, G., Frei, J., Hadjivassiliou, A., Hafkenscheid, J. C. M., Horder, M., Gella, F. J., Rej, R., Schmidt, E., Vassault, A., & Viette, V. (1992). Interlaboratory study of the IFCC method for alanine aminotransferase performed with use of a partly purified reference material. Clinical Chemistry, 38(12), 2365-2371.