Interaction of lipid nanoparticles with human epidermis and an organotypic cell culture model

Judith Kuntsche, Heike Bunjes, Alfred Fahr, Sari Pappinen, Seppo Rönkkö, Marjukka Suhonen, Arto Urtti

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Various lipid nanoparticle formulations were investigated with respect to (trans)dermal drug delivery with special regard to the mechanism of their effects on human and an organotypic cell culture epidermis. Potential alterations of stratum corneum lipid domains were studied using fluorescence assays with labeled liposomes and thermal analysis of isolated stratum corneum. Influences on the permeation of corticosterone were investigated and the occlusive properties of the nanoparticles were determined by measurements of the transepidermal water loss (TEWL). The penetration of a fluorescence dye was visualized by fluorescence microscopy of cross sections of human epidermis after incubation with cubic and solid lipid nanoparticles. Corticosterone permeation was limited when applied in matrix-type lipid nanoparticles (fat emulsion, smectic and solid lipid nanoparticles). An adhesion of solid lipid nanoparticles was clearly observed in thermal analysis as reflected by additional phase transitions probably caused by the nanoparticle matrix lipid. However, as for the other matrix-type nanoparticles, no distinct alterations of the phase transitions of the stratum corneum lipids were observed. Cubic nanoparticles led to the most predominant effect on skin permeation where the surface-active matrix lipid may act as penetration enhancer. An alteration of the stratum corneum lipids' thermal behavior as well as an interaction with fluorescence labeled liposomes was observed. Differences observed in permeation studies and thermal analysis of human and cell culture epidermis indicate that surface lipids, which are not present to the same extent in the cell culture model than in human epidermis, seem to play an important role.
OriginalsprogEngelsk
TidsskriftInternational Journal of Pharmaceutics
Vol/bind354
Udgave nummer1-2
Sider (fra-til)180-95
Antal sider16
ISSN0378-5173
DOI
StatusUdgivet - 2008

Fingeraftryk

Epidermis
Lipids
Fluorescence
Skin
Fluorescence Microscopy
Coloring Agents
Fats

Citer dette

Kuntsche, Judith ; Bunjes, Heike ; Fahr, Alfred ; Pappinen, Sari ; Rönkkö, Seppo ; Suhonen, Marjukka ; Urtti, Arto. / Interaction of lipid nanoparticles with human epidermis and an organotypic cell culture model. I: International Journal of Pharmaceutics. 2008 ; Bind 354, Nr. 1-2. s. 180-95.
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abstract = "Various lipid nanoparticle formulations were investigated with respect to (trans)dermal drug delivery with special regard to the mechanism of their effects on human and an organotypic cell culture epidermis. Potential alterations of stratum corneum lipid domains were studied using fluorescence assays with labeled liposomes and thermal analysis of isolated stratum corneum. Influences on the permeation of corticosterone were investigated and the occlusive properties of the nanoparticles were determined by measurements of the transepidermal water loss (TEWL). The penetration of a fluorescence dye was visualized by fluorescence microscopy of cross sections of human epidermis after incubation with cubic and solid lipid nanoparticles. Corticosterone permeation was limited when applied in matrix-type lipid nanoparticles (fat emulsion, smectic and solid lipid nanoparticles). An adhesion of solid lipid nanoparticles was clearly observed in thermal analysis as reflected by additional phase transitions probably caused by the nanoparticle matrix lipid. However, as for the other matrix-type nanoparticles, no distinct alterations of the phase transitions of the stratum corneum lipids were observed. Cubic nanoparticles led to the most predominant effect on skin permeation where the surface-active matrix lipid may act as penetration enhancer. An alteration of the stratum corneum lipids' thermal behavior as well as an interaction with fluorescence labeled liposomes was observed. Differences observed in permeation studies and thermal analysis of human and cell culture epidermis indicate that surface lipids, which are not present to the same extent in the cell culture model than in human epidermis, seem to play an important role.",
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Interaction of lipid nanoparticles with human epidermis and an organotypic cell culture model. / Kuntsche, Judith; Bunjes, Heike; Fahr, Alfred; Pappinen, Sari; Rönkkö, Seppo; Suhonen, Marjukka; Urtti, Arto.

I: International Journal of Pharmaceutics, Bind 354, Nr. 1-2, 2008, s. 180-95.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Interaction of lipid nanoparticles with human epidermis and an organotypic cell culture model

AU - Kuntsche, Judith

AU - Bunjes, Heike

AU - Fahr, Alfred

AU - Pappinen, Sari

AU - Rönkkö, Seppo

AU - Suhonen, Marjukka

AU - Urtti, Arto

PY - 2008

Y1 - 2008

N2 - Various lipid nanoparticle formulations were investigated with respect to (trans)dermal drug delivery with special regard to the mechanism of their effects on human and an organotypic cell culture epidermis. Potential alterations of stratum corneum lipid domains were studied using fluorescence assays with labeled liposomes and thermal analysis of isolated stratum corneum. Influences on the permeation of corticosterone were investigated and the occlusive properties of the nanoparticles were determined by measurements of the transepidermal water loss (TEWL). The penetration of a fluorescence dye was visualized by fluorescence microscopy of cross sections of human epidermis after incubation with cubic and solid lipid nanoparticles. Corticosterone permeation was limited when applied in matrix-type lipid nanoparticles (fat emulsion, smectic and solid lipid nanoparticles). An adhesion of solid lipid nanoparticles was clearly observed in thermal analysis as reflected by additional phase transitions probably caused by the nanoparticle matrix lipid. However, as for the other matrix-type nanoparticles, no distinct alterations of the phase transitions of the stratum corneum lipids were observed. Cubic nanoparticles led to the most predominant effect on skin permeation where the surface-active matrix lipid may act as penetration enhancer. An alteration of the stratum corneum lipids' thermal behavior as well as an interaction with fluorescence labeled liposomes was observed. Differences observed in permeation studies and thermal analysis of human and cell culture epidermis indicate that surface lipids, which are not present to the same extent in the cell culture model than in human epidermis, seem to play an important role.

AB - Various lipid nanoparticle formulations were investigated with respect to (trans)dermal drug delivery with special regard to the mechanism of their effects on human and an organotypic cell culture epidermis. Potential alterations of stratum corneum lipid domains were studied using fluorescence assays with labeled liposomes and thermal analysis of isolated stratum corneum. Influences on the permeation of corticosterone were investigated and the occlusive properties of the nanoparticles were determined by measurements of the transepidermal water loss (TEWL). The penetration of a fluorescence dye was visualized by fluorescence microscopy of cross sections of human epidermis after incubation with cubic and solid lipid nanoparticles. Corticosterone permeation was limited when applied in matrix-type lipid nanoparticles (fat emulsion, smectic and solid lipid nanoparticles). An adhesion of solid lipid nanoparticles was clearly observed in thermal analysis as reflected by additional phase transitions probably caused by the nanoparticle matrix lipid. However, as for the other matrix-type nanoparticles, no distinct alterations of the phase transitions of the stratum corneum lipids were observed. Cubic nanoparticles led to the most predominant effect on skin permeation where the surface-active matrix lipid may act as penetration enhancer. An alteration of the stratum corneum lipids' thermal behavior as well as an interaction with fluorescence labeled liposomes was observed. Differences observed in permeation studies and thermal analysis of human and cell culture epidermis indicate that surface lipids, which are not present to the same extent in the cell culture model than in human epidermis, seem to play an important role.

KW - Animals

KW - Calorimetry, Differential Scanning

KW - Cell Culture Techniques

KW - Corticosterone

KW - Epidermis

KW - Fluorescent Dyes

KW - Humans

KW - Keratinocytes

KW - Lipids

KW - Microscopy, Fluorescence

KW - Nanoparticles

KW - Particle Size

KW - Permeability

KW - Phase Transition

KW - Rats

KW - Skin Absorption

KW - Tissue Adhesions

U2 - 10.1016/j.ijpharm.2007.08.028

DO - 10.1016/j.ijpharm.2007.08.028

M3 - Journal article

C2 - 17920216

VL - 354

SP - 180

EP - 195

JO - International Journal of Pharmaceutics

JF - International Journal of Pharmaceutics

SN - 0378-5173

IS - 1-2

ER -