Interaction of human mesenchymal stem cells with osteopontin coated hydroxyapatite surfaces

Thomas Jensen, Alireza Dolatshahi-Pirouz, Morten Foss, Jørgen Baas, Jette Lovmand, Mogens Duch, Finn Skou Pedersen, Moustapha Kassem, Cody Bünger, Kjeld Søballe, Flemming Besenbacher

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Udgivelsesdato: 2010-Jan-1
OriginalsprogEngelsk
TidsskriftColloids and Surfaces B: Biointerfaces
Vol/bind75
Udgave nummer1
Sider (fra-til)186-93
Antal sider7
ISSN0927-7765
DOI
StatusUdgivet - 1. jan. 2010

Fingeraftryk

Osteopontin
stem cells
Durapatite
Stem cells
Mesenchymal Stromal Cells
Hydroxyapatite
interactions
locomotion
antibodies
Antibodies
attachment
orthopedics
Vinculin
milk
tissue engineering
Quartz crystal microbalances
Ellipsometry
Orthopedics
antigens
Cell membranes

Citer dette

Jensen, T., Dolatshahi-Pirouz, A., Foss, M., Baas, J., Lovmand, J., Duch, M., ... Besenbacher, F. (2010). Interaction of human mesenchymal stem cells with osteopontin coated hydroxyapatite surfaces. Colloids and Surfaces B: Biointerfaces, 75(1), 186-93. https://doi.org/10.1016/j.colsurfb.2009.08.029
Jensen, Thomas ; Dolatshahi-Pirouz, Alireza ; Foss, Morten ; Baas, Jørgen ; Lovmand, Jette ; Duch, Mogens ; Pedersen, Finn Skou ; Kassem, Moustapha ; Bünger, Cody ; Søballe, Kjeld ; Besenbacher, Flemming. / Interaction of human mesenchymal stem cells with osteopontin coated hydroxyapatite surfaces. I: Colloids and Surfaces B: Biointerfaces. 2010 ; Bind 75, Nr. 1. s. 186-93.
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title = "Interaction of human mesenchymal stem cells with osteopontin coated hydroxyapatite surfaces",
abstract = "In vitro studies of the initial attachment, spreading and motility of human bone mesenchymal stem cells have been carried out on bovine osteopontin (OPN) coated hydroxyapatite (HA) and gold (Au) model surfaces. The adsorption of OPN extracted from bovine milk was monitored by the quartz crystal microbalance with dissipation (QCM-D) and the ellipsometry techniques, and the OPN coated surfaces were further investigated by antigen-antibody interaction. It is shown that the OPN surface mass density is significantly lower and that the number of antibodies binding to the resulting OPN layers is significantly higher on the HA as compared to the Au surfaces. The initial attachment, spreading and motility of human mesenchymal stem cells show a larger cell area, a faster arrangement of vinculin in the basal cell membrane and more motile cells on the OPN coated HA surfaces as compared to the OPN coated Au surfaces and to the uncoated Au and HA surfaces. These in vitro results indicate that there may be great potential for OPN coated biomaterials, for instance as functional protein coatings or drug delivery systems on orthopaedic implants or scaffolds for tissue-engineering.",
author = "Thomas Jensen and Alireza Dolatshahi-Pirouz and Morten Foss and J{\o}rgen Baas and Jette Lovmand and Mogens Duch and Pedersen, {Finn Skou} and Moustapha Kassem and Cody B{\"u}nger and Kjeld S{\o}balle and Flemming Besenbacher",
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Jensen, T, Dolatshahi-Pirouz, A, Foss, M, Baas, J, Lovmand, J, Duch, M, Pedersen, FS, Kassem, M, Bünger, C, Søballe, K & Besenbacher, F 2010, 'Interaction of human mesenchymal stem cells with osteopontin coated hydroxyapatite surfaces', Colloids and Surfaces B: Biointerfaces, bind 75, nr. 1, s. 186-93. https://doi.org/10.1016/j.colsurfb.2009.08.029

Interaction of human mesenchymal stem cells with osteopontin coated hydroxyapatite surfaces. / Jensen, Thomas; Dolatshahi-Pirouz, Alireza; Foss, Morten; Baas, Jørgen; Lovmand, Jette; Duch, Mogens; Pedersen, Finn Skou; Kassem, Moustapha; Bünger, Cody; Søballe, Kjeld; Besenbacher, Flemming.

I: Colloids and Surfaces B: Biointerfaces, Bind 75, Nr. 1, 01.01.2010, s. 186-93.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Interaction of human mesenchymal stem cells with osteopontin coated hydroxyapatite surfaces

AU - Jensen, Thomas

AU - Dolatshahi-Pirouz, Alireza

AU - Foss, Morten

AU - Baas, Jørgen

AU - Lovmand, Jette

AU - Duch, Mogens

AU - Pedersen, Finn Skou

AU - Kassem, Moustapha

AU - Bünger, Cody

AU - Søballe, Kjeld

AU - Besenbacher, Flemming

PY - 2010/1/1

Y1 - 2010/1/1

N2 - In vitro studies of the initial attachment, spreading and motility of human bone mesenchymal stem cells have been carried out on bovine osteopontin (OPN) coated hydroxyapatite (HA) and gold (Au) model surfaces. The adsorption of OPN extracted from bovine milk was monitored by the quartz crystal microbalance with dissipation (QCM-D) and the ellipsometry techniques, and the OPN coated surfaces were further investigated by antigen-antibody interaction. It is shown that the OPN surface mass density is significantly lower and that the number of antibodies binding to the resulting OPN layers is significantly higher on the HA as compared to the Au surfaces. The initial attachment, spreading and motility of human mesenchymal stem cells show a larger cell area, a faster arrangement of vinculin in the basal cell membrane and more motile cells on the OPN coated HA surfaces as compared to the OPN coated Au surfaces and to the uncoated Au and HA surfaces. These in vitro results indicate that there may be great potential for OPN coated biomaterials, for instance as functional protein coatings or drug delivery systems on orthopaedic implants or scaffolds for tissue-engineering.

AB - In vitro studies of the initial attachment, spreading and motility of human bone mesenchymal stem cells have been carried out on bovine osteopontin (OPN) coated hydroxyapatite (HA) and gold (Au) model surfaces. The adsorption of OPN extracted from bovine milk was monitored by the quartz crystal microbalance with dissipation (QCM-D) and the ellipsometry techniques, and the OPN coated surfaces were further investigated by antigen-antibody interaction. It is shown that the OPN surface mass density is significantly lower and that the number of antibodies binding to the resulting OPN layers is significantly higher on the HA as compared to the Au surfaces. The initial attachment, spreading and motility of human mesenchymal stem cells show a larger cell area, a faster arrangement of vinculin in the basal cell membrane and more motile cells on the OPN coated HA surfaces as compared to the OPN coated Au surfaces and to the uncoated Au and HA surfaces. These in vitro results indicate that there may be great potential for OPN coated biomaterials, for instance as functional protein coatings or drug delivery systems on orthopaedic implants or scaffolds for tissue-engineering.

U2 - 10.1016/j.colsurfb.2009.08.029

DO - 10.1016/j.colsurfb.2009.08.029

M3 - Journal article

C2 - 19783129

VL - 75

SP - 186

EP - 193

JO - Colloids and Surfaces B: Biointerfaces

JF - Colloids and Surfaces B: Biointerfaces

SN - 0927-7765

IS - 1

ER -