@article{991ea110fa0711deaefb000ea68e967b,
title = "Insulin and IGF-II, but not IGF-I, stimulate the in vitro regeneration of adult frog sciatic sensory axons",
abstract = "We used the in vitro regenerating frog sciatic nerve to look for effects of insulin and insulin-like growth factors I and II (IGF-I, IGF-II) on regeneration of sensory axons and on injury induced support cell proliferation in the outgrowth region. In nerves cultured for 11 days, a physiological dose (10 ng/ml, approximately 2 nM) of insulin or IGF-II increased ganglionic protein synthesis (by 20% and 50%, respectively) as well as the level of newly formed, radiolabelled axonal material distal to a crush injury (both by 80%), compared to untreated, paired controls. In addition, insulin increased the outgrowth distance of the furthest regenerating sensory axons by 10%. The preparation was particularly sensitive to insulin during the first 5 days of culturing. Furthermore, both insulin and IGF-II were found to inhibit proliferation of support cells in the outgrowth region in a manner suggesting effects via their individual receptors. The inhibition, about 30%, was observable after 4 but not 11 days in culture. It is not clear if this reflects a stimulated differentiation of some cells. By contrast, IGF-I lacked effects on both regeneration and proliferation. In conclusion, the results suggest that insulin and IGF-II are involved in the regulation of peripheral nerve regeneration.",
keywords = "Animals, Axons, Biological Transport, Cell Division, Culture Media, Serum-Free, DNA, Ganglia, Sensory, Insulin, Insulin-Like Growth Factor I, Insulin-Like Growth Factor II, Mannosephosphates, Nerve Regeneration, Nerve Tissue Proteins, Rana temporaria, Sciatic Nerve",
author = "M Edbladh and Svenningsen, {{\AA}sa Fex} and Ekstr{\"o}m, {P A} and A Edstr{\"o}m",
year = "1994",
language = "English",
volume = "641",
pages = "76--82",
journal = "Brain Research",
issn = "0006-8993",
publisher = "Elsevier",
number = "1",
}