Inhibition of yeast growth during long term exposure to laser light around 1064 nm

  • Thomas Aabo
  • , Ivan R. Perch-Nielsen
  • , Jeppe Seidelin Dam
  • , Darwin Palima
  • , Henrik Siegumfeldt
  • , Jesper Glückstad
  • , Nils Arneborg

Publikation: Kapitel i bog/rapport/konference-proceedingKonferencebidrag i proceedingsForskningpeer review

Abstract

We have studied the effect of a 1070 nm continuous wave Ytterbium fiber laser on exponentially growing Saccharomyces cerevisiae yeast cells over a span of 4 hours. The cells were immobilized onto Concanavalin A covered microscope slides and the growth was measured using the area increase of the cells in 2D. Using a continuous dual beam plane wave with a uniform spatial intensity distribution, we found that a continuous radiant flux through a single cell as low as 0.5 mW in 1.5 hours significantly changed the growth and division rate of S. cerevisiae. With the dual beam setup used we were able to successfully manipulate single S. cerevisiae cells in 3 dimensions with a minimum flux thorough the cell of 3.5 mW. In the regime investigated from 0.7 mW to 2.6 mW we found no threshold for the photo damage, but rather a continuous response to the increased accumulated dose.
OriginalsprogEngelsk
TitelComplex Light and Optical Forces III
ForlagSPIE - International Society for Optical Engineering
Publikationsdato2009
Artikelnummer722706
DOI
StatusUdgivet - 2009
Udgivet eksterntJa
BegivenhedSPIE OPTO: INTEGRATED OPTOELECTRONIC DEVICES - San Jose, USA
Varighed: 24. jan. 200929. jan. 2009

Konference

KonferenceSPIE OPTO: INTEGRATED OPTOELECTRONIC DEVICES
Land/OmrådeUSA
BySan Jose
Periode24/01/200929/01/2009
NavnProceedings of SPIE, the International Society for Optical Engineering
Vol/bind7227
ISSN0277-786X

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