Inhibition of pulmonary surfactant adsorption by serum and the mechanisms of reversal by hydrophilic polymers: theory.

Joseph A Zasadzinski, T F Alig, Coralie Alonso, Jorge Bernardino de la Serna, Jesus Perez-Gil, H William Taeusch

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

A theory based on the Smolukowski analysis of colloid stability shows that the presence of charged, surface-active serum proteins at the alveolar air-liquid interface can severely reduce or eliminate the adsorption of lung surfactant from the subphase to the interface, consistent with the observations reported in the companion article (pages 1769-1779). Adding nonadsorbing, hydrophilic polymers to the subphase provides a depletion attraction between the surfactant aggregates and the interface, which can overcome the steric and electrostatic resistance to adsorption induced by serum. The depletion force increases with polymer concentration as well as with polymer molecular weight. Increasing the surfactant concentration has a much smaller effect than adding polymer, as is observed. Natural hydrophilic polymers, like the SP-A present in native surfactant, or hyaluronan, normally present in the alveolar fluids, can enhance adsorption in the presence of serum to eliminate inactivation.
Udgivelsesdato: 2005-Sep
OriginalsprogEngelsk
TidsskriftBiophysical Journal
Vol/bind89
Udgave nummer3
Sider (fra-til)1621-9
Antal sider8
ISSN0006-3495
DOI
StatusUdgivet - 1. sep. 2005

Citer dette

Zasadzinski, J. A., Alig, T. F., Alonso, C., de la Serna, J. B., Perez-Gil, J., & Taeusch, H. W. (2005). Inhibition of pulmonary surfactant adsorption by serum and the mechanisms of reversal by hydrophilic polymers: theory. Biophysical Journal, 89(3), 1621-9. https://doi.org/10.1529/biophysj.105.062646
Zasadzinski, Joseph A ; Alig, T F ; Alonso, Coralie ; de la Serna, Jorge Bernardino ; Perez-Gil, Jesus ; Taeusch, H William. / Inhibition of pulmonary surfactant adsorption by serum and the mechanisms of reversal by hydrophilic polymers: theory. I: Biophysical Journal. 2005 ; Bind 89, Nr. 3. s. 1621-9.
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abstract = "A theory based on the Smolukowski analysis of colloid stability shows that the presence of charged, surface-active serum proteins at the alveolar air-liquid interface can severely reduce or eliminate the adsorption of lung surfactant from the subphase to the interface, consistent with the observations reported in the companion article (pages 1769-1779). Adding nonadsorbing, hydrophilic polymers to the subphase provides a depletion attraction between the surfactant aggregates and the interface, which can overcome the steric and electrostatic resistance to adsorption induced by serum. The depletion force increases with polymer concentration as well as with polymer molecular weight. Increasing the surfactant concentration has a much smaller effect than adding polymer, as is observed. Natural hydrophilic polymers, like the SP-A present in native surfactant, or hyaluronan, normally present in the alveolar fluids, can enhance adsorption in the presence of serum to eliminate inactivation. Udgivelsesdato: 2005-Sep",
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Zasadzinski, JA, Alig, TF, Alonso, C, de la Serna, JB, Perez-Gil, J & Taeusch, HW 2005, 'Inhibition of pulmonary surfactant adsorption by serum and the mechanisms of reversal by hydrophilic polymers: theory.', Biophysical Journal, bind 89, nr. 3, s. 1621-9. https://doi.org/10.1529/biophysj.105.062646

Inhibition of pulmonary surfactant adsorption by serum and the mechanisms of reversal by hydrophilic polymers: theory. / Zasadzinski, Joseph A; Alig, T F; Alonso, Coralie; de la Serna, Jorge Bernardino; Perez-Gil, Jesus; Taeusch, H William.

I: Biophysical Journal, Bind 89, Nr. 3, 01.09.2005, s. 1621-9.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Inhibition of pulmonary surfactant adsorption by serum and the mechanisms of reversal by hydrophilic polymers: theory.

AU - Zasadzinski, Joseph A

AU - Alig, T F

AU - Alonso, Coralie

AU - de la Serna, Jorge Bernardino

AU - Perez-Gil, Jesus

AU - Taeusch, H William

PY - 2005/9/1

Y1 - 2005/9/1

N2 - A theory based on the Smolukowski analysis of colloid stability shows that the presence of charged, surface-active serum proteins at the alveolar air-liquid interface can severely reduce or eliminate the adsorption of lung surfactant from the subphase to the interface, consistent with the observations reported in the companion article (pages 1769-1779). Adding nonadsorbing, hydrophilic polymers to the subphase provides a depletion attraction between the surfactant aggregates and the interface, which can overcome the steric and electrostatic resistance to adsorption induced by serum. The depletion force increases with polymer concentration as well as with polymer molecular weight. Increasing the surfactant concentration has a much smaller effect than adding polymer, as is observed. Natural hydrophilic polymers, like the SP-A present in native surfactant, or hyaluronan, normally present in the alveolar fluids, can enhance adsorption in the presence of serum to eliminate inactivation. Udgivelsesdato: 2005-Sep

AB - A theory based on the Smolukowski analysis of colloid stability shows that the presence of charged, surface-active serum proteins at the alveolar air-liquid interface can severely reduce or eliminate the adsorption of lung surfactant from the subphase to the interface, consistent with the observations reported in the companion article (pages 1769-1779). Adding nonadsorbing, hydrophilic polymers to the subphase provides a depletion attraction between the surfactant aggregates and the interface, which can overcome the steric and electrostatic resistance to adsorption induced by serum. The depletion force increases with polymer concentration as well as with polymer molecular weight. Increasing the surfactant concentration has a much smaller effect than adding polymer, as is observed. Natural hydrophilic polymers, like the SP-A present in native surfactant, or hyaluronan, normally present in the alveolar fluids, can enhance adsorption in the presence of serum to eliminate inactivation. Udgivelsesdato: 2005-Sep

KW - Adsorption

KW - Biological Products

KW - Biophysics

KW - Blood Proteins

KW - Electrostatics

KW - Humans

KW - Hyaluronic Acid

KW - Inflammation

KW - Models, Statistical

KW - Models, Theoretical

KW - Molecular Weight

KW - Osmotic Pressure

KW - Phospholipids

KW - Polymers

KW - Pressure

KW - Proteins

KW - Pulmonary Alveoli

KW - Pulmonary Surfactants

KW - Surface Properties

KW - Surface Tension

KW - Surface-Active Agents

KW - Temperature

KW - Time Factors

U2 - 10.1529/biophysj.105.062646

DO - 10.1529/biophysj.105.062646

M3 - Journal article

VL - 89

SP - 1621

EP - 1629

JO - Biophysical Journal

JF - Biophysical Journal

SN - 0006-3495

IS - 3

ER -