Influence of Very Early Exposure of Cefuroxime on Gut Microbiota Composition of Infants Born by Caesarean Section

Karen Angeliki Krogfelt, Shamrulazhar Bin Shamzir Kamal, Nana Hyldig, Lukasz Krych, Gorm Greisen, Gitte Zachariassen, Dennis Sandris Nielsen

Publikation: Konferencebidrag uden forlag/tidsskriftKonferenceabstrakt til konferenceForskningpeer review


Background: Cefuroxime is a broad-spectrum cephalosporin antibiotic. It is standard caesarean section (CS) procedure at many hospitals to administer the mother a single prophylactic dose (1500mg) before skin incision and hence before the umbilical cord is cut, indirectly exposing the foetus to the antibiotic. It remains unknown whether this very early exposure to antibiotics may influence gut microbiota (GM) colonization and bacterial antibiotic resistance. Objective: To investigate the influence of very early exposure of cefuroxime on GM composition and bacterial antibiotic resistance on term infants born by CS. Methods: 42 pregnant women (BMI<30) scheduled for elective CS were recruited at Odense University Hospital, Denmark. The mothers were randomly assigned to either receiving cefuroxime before skin incision or immediately after the umbilical cord were cut. Faecal samples were collected from all infants at 10 days. GM composition were determined by MiSeq-based tag-encoded 16S rRNA gene targeted high throughput amplicon sequencing. Bacteria were cultured on selective and non-selective agar plates (aerobically and anaerobically) from the faecal samples and the bacterial strains were isolated. Antimicrobial susceptibility tests were performed. Conclusion: The GM of both groups of infants were dominated by the genera Bifidobacterium, Clostridium, Veillonella and family Enterobacteriaceae. Testing with analysis of similarity (ANOSIM) on both unweighted and weighted UniFrac distance matrices revealed no major differences between the 2 groups. Enterobacteriaceae, Enterococcus spp., and Staphylococcus spp. were the major groups of bacteria isolated from both groups of infants. Antimicrobial susceptibility was performed with no differences were seen between the two treatments groups. In conclusion, short, early life exposure to cefuroxime does not have major effects on GM colonization and bacterial antibiotic resistance traits in infants born by CS.
StatusUdgivet - 2016