Influence of the OGG1 Ser326Cys polymorphism on oxidatively damaged DNA and repair activity

Annie Aarup Jensen, Mille Løhr, Louise Eriksen, Morten Grønbæk, Elad Dorry, Steffen Loft, Peter Frost Møller

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Abstrakt

Oxidatively damaged DNA base lesions are considered to be mainly repaired by 8-oxoguanine DNA glycosylase (OGG1) mediated pathways. We investigated the effect of the OGG1 Ser326Cys polymorphism on the level and repair of oxidatively damaged DNA in mononuclear blood cells (MNBC) by means of the comet assay. We collected blood samples from 1,019 healthy subjects and genotyped for the OGG1 Ser326Cys polymorphism. We found 49 subjects homozygous for the variant genotype (Cys/Cys) and selected same numbers of age-matched subjects with the heterozygous (Ser/Cys) and homozygous wild-type genotype (Ser/Ser). Carriers of the Cys/Cys genotype had higher levels of formamidopyrimidine DNA glycosylase (FPG) sensitive sites in MNBC (0.31 ± 0.03 lesions/10(6)bp) compared to Ser/Ser (0.19 ± 0.02 lesions/10(6)bp, P
OriginalsprogEngelsk
TidsskriftFree Radical Biology & Medicine
Vol/bind52
Udgave nummer1
Sider (fra-til)118-25
Antal sider8
ISSN0891-5849
DOI
StatusUdgivet - 2012

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