Influence of stabilizer systems on the properties and phase behavior of supercooled smectic nanoparticles

Judith Kuntsche, Michel H J Koch, Frank Steiniger, Heike Bunjes

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Colloidal dispersions of cholesterol esters in the supercooled smectic state (supercooled smectic nanoparticles) are potential novel carrier systems for poorly water soluble drugs. As the supercooled smectic state is metastable, evaluation of its stability and of parameters influencing it is essential. In the present study, the effect of different emulsifiers on the stability of the supercooled smectic state of cholesteryl myristate (CM) nanoparticles and their crystallization was investigated. Nanoparticles were prepared by high-pressure melt homogenization and characterized by dynamic light scattering (DLS), laser diffraction combined with polarization intensity differential scattering (LD-PIDS), synchrotron radiation small-angle X-ray scattering (SAXS), differential scanning calorimetry (DSC) and transmission electron microscopy (TEM, negative staining and cryo-preparation). The various stabilizers resulted in clear differences in the crystallization behavior of the nanoparticles: stabilizers containing a fatty acid chain in their molecule (e.g. phospholipids, sodium oleate and sucrose monolaurate) induced a multiple crystallization event accompanied by a comparatively high recrystallization tendency. In contrast, the recrystallization tendency of nanoparticles stabilized with polymers (e.g. gelatin polysuccinate, poloxamer, poloxamine, polyvinyl alcohol) and sodium glycocholate was much lower and a single crystallization event was observed. The high stability against recrystallization during storage of smectic nanoparticles stabilized with polysorbate 80 in spite of the presence of a fatty acyl group in the molecule suggests that the polar head group (e.g. polyethylene glycol chains) of the emulsifier may also play a significant role.
OriginalsprogEngelsk
TidsskriftJournal of Colloid and Interface Science
Vol/bind350
Udgave nummer1
Sider (fra-til)229-239
ISSN0021-9797
DOI
StatusUdgivet - 2010

Fingeraftryk

Phase behavior
Crystallization
Nanoparticles
sucrose monolaurate
Sodium
Glycocholic Acid
Transmission electron microscopy
Polyvinyl Alcohol
Poloxamer
Molecules
Polysorbates
Cholesterol Esters
Polyvinyl alcohols
Cholesterol
Phospholipids
Sugar (sucrose)
Dynamic light scattering
Gelatin
Synchrotron radiation
X ray scattering

Citer dette

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title = "Influence of stabilizer systems on the properties and phase behavior of supercooled smectic nanoparticles",
abstract = "Colloidal dispersions of cholesterol esters in the supercooled smectic state (supercooled smectic nanoparticles) are potential novel carrier systems for poorly water soluble drugs. As the supercooled smectic state is metastable, evaluation of its stability and of parameters influencing it is essential. In the present study, the effect of different emulsifiers on the stability of the supercooled smectic state of cholesteryl myristate (CM) nanoparticles and their crystallization was investigated. Nanoparticles were prepared by high-pressure melt homogenization and characterized by dynamic light scattering (DLS), laser diffraction combined with polarization intensity differential scattering (LD-PIDS), synchrotron radiation small-angle X-ray scattering (SAXS), differential scanning calorimetry (DSC) and transmission electron microscopy (TEM, negative staining and cryo-preparation). The various stabilizers resulted in clear differences in the crystallization behavior of the nanoparticles: stabilizers containing a fatty acid chain in their molecule (e.g. phospholipids, sodium oleate and sucrose monolaurate) induced a multiple crystallization event accompanied by a comparatively high recrystallization tendency. In contrast, the recrystallization tendency of nanoparticles stabilized with polymers (e.g. gelatin polysuccinate, poloxamer, poloxamine, polyvinyl alcohol) and sodium glycocholate was much lower and a single crystallization event was observed. The high stability against recrystallization during storage of smectic nanoparticles stabilized with polysorbate 80 in spite of the presence of a fatty acyl group in the molecule suggests that the polar head group (e.g. polyethylene glycol chains) of the emulsifier may also play a significant role.",
keywords = "Calorimetry, Differential Scanning, Drug Stability, Drug Storage, Emulsifying Agents, Liquid Crystals, Microscopy, Electron, Transmission, Molecular Structure, Nanoparticles, Particle Size, Phase Transition",
author = "Judith Kuntsche and Koch, {Michel H J} and Frank Steiniger and Heike Bunjes",
note = "Copyright 2010 Elsevier Inc. All rights reserved.",
year = "2010",
doi = "10.1016/j.jcis.2010.06.018",
language = "English",
volume = "350",
pages = "229--239",
journal = "Journal of Colloid and Interface Science",
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Influence of stabilizer systems on the properties and phase behavior of supercooled smectic nanoparticles. / Kuntsche, Judith; Koch, Michel H J; Steiniger, Frank; Bunjes, Heike.

I: Journal of Colloid and Interface Science, Bind 350, Nr. 1, 2010, s. 229-239.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Influence of stabilizer systems on the properties and phase behavior of supercooled smectic nanoparticles

AU - Kuntsche, Judith

AU - Koch, Michel H J

AU - Steiniger, Frank

AU - Bunjes, Heike

N1 - Copyright 2010 Elsevier Inc. All rights reserved.

PY - 2010

Y1 - 2010

N2 - Colloidal dispersions of cholesterol esters in the supercooled smectic state (supercooled smectic nanoparticles) are potential novel carrier systems for poorly water soluble drugs. As the supercooled smectic state is metastable, evaluation of its stability and of parameters influencing it is essential. In the present study, the effect of different emulsifiers on the stability of the supercooled smectic state of cholesteryl myristate (CM) nanoparticles and their crystallization was investigated. Nanoparticles were prepared by high-pressure melt homogenization and characterized by dynamic light scattering (DLS), laser diffraction combined with polarization intensity differential scattering (LD-PIDS), synchrotron radiation small-angle X-ray scattering (SAXS), differential scanning calorimetry (DSC) and transmission electron microscopy (TEM, negative staining and cryo-preparation). The various stabilizers resulted in clear differences in the crystallization behavior of the nanoparticles: stabilizers containing a fatty acid chain in their molecule (e.g. phospholipids, sodium oleate and sucrose monolaurate) induced a multiple crystallization event accompanied by a comparatively high recrystallization tendency. In contrast, the recrystallization tendency of nanoparticles stabilized with polymers (e.g. gelatin polysuccinate, poloxamer, poloxamine, polyvinyl alcohol) and sodium glycocholate was much lower and a single crystallization event was observed. The high stability against recrystallization during storage of smectic nanoparticles stabilized with polysorbate 80 in spite of the presence of a fatty acyl group in the molecule suggests that the polar head group (e.g. polyethylene glycol chains) of the emulsifier may also play a significant role.

AB - Colloidal dispersions of cholesterol esters in the supercooled smectic state (supercooled smectic nanoparticles) are potential novel carrier systems for poorly water soluble drugs. As the supercooled smectic state is metastable, evaluation of its stability and of parameters influencing it is essential. In the present study, the effect of different emulsifiers on the stability of the supercooled smectic state of cholesteryl myristate (CM) nanoparticles and their crystallization was investigated. Nanoparticles were prepared by high-pressure melt homogenization and characterized by dynamic light scattering (DLS), laser diffraction combined with polarization intensity differential scattering (LD-PIDS), synchrotron radiation small-angle X-ray scattering (SAXS), differential scanning calorimetry (DSC) and transmission electron microscopy (TEM, negative staining and cryo-preparation). The various stabilizers resulted in clear differences in the crystallization behavior of the nanoparticles: stabilizers containing a fatty acid chain in their molecule (e.g. phospholipids, sodium oleate and sucrose monolaurate) induced a multiple crystallization event accompanied by a comparatively high recrystallization tendency. In contrast, the recrystallization tendency of nanoparticles stabilized with polymers (e.g. gelatin polysuccinate, poloxamer, poloxamine, polyvinyl alcohol) and sodium glycocholate was much lower and a single crystallization event was observed. The high stability against recrystallization during storage of smectic nanoparticles stabilized with polysorbate 80 in spite of the presence of a fatty acyl group in the molecule suggests that the polar head group (e.g. polyethylene glycol chains) of the emulsifier may also play a significant role.

KW - Calorimetry, Differential Scanning

KW - Drug Stability

KW - Drug Storage

KW - Emulsifying Agents

KW - Liquid Crystals

KW - Microscopy, Electron, Transmission

KW - Molecular Structure

KW - Nanoparticles

KW - Particle Size

KW - Phase Transition

U2 - 10.1016/j.jcis.2010.06.018

DO - 10.1016/j.jcis.2010.06.018

M3 - Journal article

C2 - 20619846

VL - 350

SP - 229

EP - 239

JO - Journal of Colloid and Interface Science

JF - Journal of Colloid and Interface Science

SN - 0021-9797

IS - 1

ER -