Influence of pH and redox on mobilization of inositol hexakisphosphate from oligotrophic lake sediment

Kasper Reitzel*, Henning S. Jensen, Benjamin L. Turner, Charlotte Jørgensen

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Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

It has been suggested that inositol hexakisphosphate (IP6) contributes to the release of phosphorus (P) from lake sediments, but a mechanistic understanding remains elusive. We investigated the potential mobilization and mineralization of myo- and scyllo-IP6 from the sediment of an oligotrophic Danish lake known to contain high concentrations of inositol phosphates. Solution 31P NMR spectroscopy was used to determine changes in myo- and scyllo-IP6 in laboratory microcosms incubated under either oxic or anoxic conditions. In addition, we incubated sediment slurries adjusted to pH between 4.9 and 6.6, with and without addition of myo-IP6, and induced redox changes by adding starch and sulfate. We observed no significant changes in myo- or scyllo-IP6 after 1 year of incubation under anaerobic conditions. A sequential extraction procedure revealed that one half of the added myo-IP6 was recovered in the humic acid fraction (acid-insoluble organic matter) and the other half in the fulvic acid fraction (acid-soluble organic matter). Reduction in redox potential by starch addition did not mobilize myo-IP6, but myo-IP6 bound to humic acids was released to the pore water when the pH was increased to ≥ 5.8. This pH-induced mobilization of IP6 occurred in parallel with increases in dissolved iron and organic matter, suggesting the release of IP6 bound to humic acids through metal bridges. We conclude that myo-IP6 mobilization from this oligotrophic lake sediment is driven by changes in pH rather than by changes in the redox potential.

OriginalsprogEngelsk
TidsskriftBiogeochemistry
Vol/bind140
Udgave nummer1
Sider (fra-til)15-30
ISSN0168-2563
DOI
StatusUdgivet - 1. aug. 2018

Fingeraftryk

Phytic Acid
Humic Substances
humic acid
Lakes
mobilization
lacustrine deposit
Sediments
Biological materials
redox potential
organic matter
starch
anoxic conditions
Starch
acid
fulvic acid
oxic conditions
microcosm
sediment
Inositol Phosphates
nuclear magnetic resonance

Citer dette

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title = "Influence of pH and redox on mobilization of inositol hexakisphosphate from oligotrophic lake sediment",
abstract = "It has been suggested that inositol hexakisphosphate (IP6) contributes to the release of phosphorus (P) from lake sediments, but a mechanistic understanding remains elusive. We investigated the potential mobilization and mineralization of myo- and scyllo-IP6 from the sediment of an oligotrophic Danish lake known to contain high concentrations of inositol phosphates. Solution 31P NMR spectroscopy was used to determine changes in myo- and scyllo-IP6 in laboratory microcosms incubated under either oxic or anoxic conditions. In addition, we incubated sediment slurries adjusted to pH between 4.9 and 6.6, with and without addition of myo-IP6, and induced redox changes by adding starch and sulfate. We observed no significant changes in myo- or scyllo-IP6 after 1 year of incubation under anaerobic conditions. A sequential extraction procedure revealed that one half of the added myo-IP6 was recovered in the humic acid fraction (acid-insoluble organic matter) and the other half in the fulvic acid fraction (acid-soluble organic matter). Reduction in redox potential by starch addition did not mobilize myo-IP6, but myo-IP6 bound to humic acids was released to the pore water when the pH was increased to ≥ 5.8. This pH-induced mobilization of IP6 occurred in parallel with increases in dissolved iron and organic matter, suggesting the release of IP6 bound to humic acids through metal bridges. We conclude that myo-IP6 mobilization from this oligotrophic lake sediment is driven by changes in pH rather than by changes in the redox potential.",
keywords = "d-chiro-inositol hexakisphosphate, myo-inositol hexakisphosphate, neo-inositol hexakisphosphate, scyllo-inositol hexakisphosphate, Sequential phosphorus extraction, Solution P NMR spectroscopy",
author = "Kasper Reitzel and Jensen, {Henning S.} and Turner, {Benjamin L.} and Charlotte J{\o}rgensen",
year = "2018",
month = "8",
day = "1",
doi = "10.1007/s10533-018-0468-4",
language = "English",
volume = "140",
pages = "15--30",
journal = "Biogeochemistry",
issn = "0168-2563",
publisher = "Springer",
number = "1",

}

Influence of pH and redox on mobilization of inositol hexakisphosphate from oligotrophic lake sediment. / Reitzel, Kasper; Jensen, Henning S.; Turner, Benjamin L.; Jørgensen, Charlotte.

I: Biogeochemistry, Bind 140, Nr. 1, 01.08.2018, s. 15-30.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Influence of pH and redox on mobilization of inositol hexakisphosphate from oligotrophic lake sediment

AU - Reitzel, Kasper

AU - Jensen, Henning S.

AU - Turner, Benjamin L.

AU - Jørgensen, Charlotte

PY - 2018/8/1

Y1 - 2018/8/1

N2 - It has been suggested that inositol hexakisphosphate (IP6) contributes to the release of phosphorus (P) from lake sediments, but a mechanistic understanding remains elusive. We investigated the potential mobilization and mineralization of myo- and scyllo-IP6 from the sediment of an oligotrophic Danish lake known to contain high concentrations of inositol phosphates. Solution 31P NMR spectroscopy was used to determine changes in myo- and scyllo-IP6 in laboratory microcosms incubated under either oxic or anoxic conditions. In addition, we incubated sediment slurries adjusted to pH between 4.9 and 6.6, with and without addition of myo-IP6, and induced redox changes by adding starch and sulfate. We observed no significant changes in myo- or scyllo-IP6 after 1 year of incubation under anaerobic conditions. A sequential extraction procedure revealed that one half of the added myo-IP6 was recovered in the humic acid fraction (acid-insoluble organic matter) and the other half in the fulvic acid fraction (acid-soluble organic matter). Reduction in redox potential by starch addition did not mobilize myo-IP6, but myo-IP6 bound to humic acids was released to the pore water when the pH was increased to ≥ 5.8. This pH-induced mobilization of IP6 occurred in parallel with increases in dissolved iron and organic matter, suggesting the release of IP6 bound to humic acids through metal bridges. We conclude that myo-IP6 mobilization from this oligotrophic lake sediment is driven by changes in pH rather than by changes in the redox potential.

AB - It has been suggested that inositol hexakisphosphate (IP6) contributes to the release of phosphorus (P) from lake sediments, but a mechanistic understanding remains elusive. We investigated the potential mobilization and mineralization of myo- and scyllo-IP6 from the sediment of an oligotrophic Danish lake known to contain high concentrations of inositol phosphates. Solution 31P NMR spectroscopy was used to determine changes in myo- and scyllo-IP6 in laboratory microcosms incubated under either oxic or anoxic conditions. In addition, we incubated sediment slurries adjusted to pH between 4.9 and 6.6, with and without addition of myo-IP6, and induced redox changes by adding starch and sulfate. We observed no significant changes in myo- or scyllo-IP6 after 1 year of incubation under anaerobic conditions. A sequential extraction procedure revealed that one half of the added myo-IP6 was recovered in the humic acid fraction (acid-insoluble organic matter) and the other half in the fulvic acid fraction (acid-soluble organic matter). Reduction in redox potential by starch addition did not mobilize myo-IP6, but myo-IP6 bound to humic acids was released to the pore water when the pH was increased to ≥ 5.8. This pH-induced mobilization of IP6 occurred in parallel with increases in dissolved iron and organic matter, suggesting the release of IP6 bound to humic acids through metal bridges. We conclude that myo-IP6 mobilization from this oligotrophic lake sediment is driven by changes in pH rather than by changes in the redox potential.

KW - d-chiro-inositol hexakisphosphate

KW - myo-inositol hexakisphosphate

KW - neo-inositol hexakisphosphate

KW - scyllo-inositol hexakisphosphate

KW - Sequential phosphorus extraction

KW - Solution P NMR spectroscopy

U2 - 10.1007/s10533-018-0468-4

DO - 10.1007/s10533-018-0468-4

M3 - Journal article

VL - 140

SP - 15

EP - 30

JO - Biogeochemistry

JF - Biogeochemistry

SN - 0168-2563

IS - 1

ER -