Induction of quiescence (G0) in bone marrow stromal stem cells enhances their stem cell characteristics

Mohammad Rumman, Abhijit Majumder, Linda Harkness, Balu Venugopal, M. B. Vinay, Malini S. Pillai, Moustapha Kassem*, Jyotsna Dhawan

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Resumé

Several studies have suggested that bone marrow stromal steam cells (BMSC) exist in a quiescent state (G0) within the in vivo niche; however, an explicit analysis of the biology of G0 state-BMSC has not been reported. We hypothesized that induction of G0 in BMSC might enhance their stem cell properties. Thus, we induced quiescence in BMSC in vitro by (a) suspension culture in a viscous medium or (b) culture on soft polyacrylamide substrate; and examined their molecular and functional phenotype. Induction of G0 was confirmed by bromo-deoxyuridine (BrdU) labelling and analysis of cell cycle gene expression. Upon reactivation and re-entry into cell cycle, G0 state-BMSC exhibited enhanced clonogenic self-renewal, preferential differentiation into osteoblastic rather than adipocytic cells and increased ectopic bone formation when implanted subcutaneously in vivo in immune-deficient mice, compared to asynchronous proliferating (pre-G0) BMSC. Global gene expression profiling revealed reprogramming of the transcriptome during G0 state including significant alterations in relevant pathways and expression of secreted factors, suggesting altered autocrine and paracrine signaling by G0 state-BMSC and a possible mechanism for enhanced bone formation. G0 state-BMSC might provide a clinically relevant model for understanding the in vivo biology of BMSC.

OriginalsprogEngelsk
TidsskriftStem Cell Research
Vol/bind30
Sider (fra-til)69-80
ISSN1873-5061
DOI
StatusUdgivet - jul. 2018

Fingeraftryk

Mesenchymal Stromal Cells
Osteogenesis
Autocrine Communication
Paracrine Communication
cdc Genes
Gene Expression Profiling
Transcriptome
Cell Cycle

Citer dette

Rumman, M., Majumder, A., Harkness, L., Venugopal, B., Vinay, M. B., Pillai, M. S., ... Dhawan, J. (2018). Induction of quiescence (G0) in bone marrow stromal stem cells enhances their stem cell characteristics. Stem Cell Research, 30, 69-80. https://doi.org/10.1016/j.scr.2018.05.010
Rumman, Mohammad ; Majumder, Abhijit ; Harkness, Linda ; Venugopal, Balu ; Vinay, M. B. ; Pillai, Malini S. ; Kassem, Moustapha ; Dhawan, Jyotsna. / Induction of quiescence (G0) in bone marrow stromal stem cells enhances their stem cell characteristics. I: Stem Cell Research. 2018 ; Bind 30. s. 69-80.
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title = "Induction of quiescence (G0) in bone marrow stromal stem cells enhances their stem cell characteristics",
abstract = "Several studies have suggested that bone marrow stromal steam cells (BMSC) exist in a quiescent state (G0) within the in vivo niche; however, an explicit analysis of the biology of G0 state-BMSC has not been reported. We hypothesized that induction of G0 in BMSC might enhance their stem cell properties. Thus, we induced quiescence in BMSC in vitro by (a) suspension culture in a viscous medium or (b) culture on soft polyacrylamide substrate; and examined their molecular and functional phenotype. Induction of G0 was confirmed by bromo-deoxyuridine (BrdU) labelling and analysis of cell cycle gene expression. Upon reactivation and re-entry into cell cycle, G0 state-BMSC exhibited enhanced clonogenic self-renewal, preferential differentiation into osteoblastic rather than adipocytic cells and increased ectopic bone formation when implanted subcutaneously in vivo in immune-deficient mice, compared to asynchronous proliferating (pre-G0) BMSC. Global gene expression profiling revealed reprogramming of the transcriptome during G0 state including significant alterations in relevant pathways and expression of secreted factors, suggesting altered autocrine and paracrine signaling by G0 state-BMSC and a possible mechanism for enhanced bone formation. G0 state-BMSC might provide a clinically relevant model for understanding the in vivo biology of BMSC.",
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author = "Mohammad Rumman and Abhijit Majumder and Linda Harkness and Balu Venugopal and Vinay, {M. B.} and Pillai, {Malini S.} and Moustapha Kassem and Jyotsna Dhawan",
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Rumman, M, Majumder, A, Harkness, L, Venugopal, B, Vinay, MB, Pillai, MS, Kassem, M & Dhawan, J 2018, 'Induction of quiescence (G0) in bone marrow stromal stem cells enhances their stem cell characteristics', Stem Cell Research, bind 30, s. 69-80. https://doi.org/10.1016/j.scr.2018.05.010

Induction of quiescence (G0) in bone marrow stromal stem cells enhances their stem cell characteristics. / Rumman, Mohammad; Majumder, Abhijit; Harkness, Linda; Venugopal, Balu; Vinay, M. B.; Pillai, Malini S.; Kassem, Moustapha; Dhawan, Jyotsna.

I: Stem Cell Research, Bind 30, 07.2018, s. 69-80.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Induction of quiescence (G0) in bone marrow stromal stem cells enhances their stem cell characteristics

AU - Rumman, Mohammad

AU - Majumder, Abhijit

AU - Harkness, Linda

AU - Venugopal, Balu

AU - Vinay, M. B.

AU - Pillai, Malini S.

AU - Kassem, Moustapha

AU - Dhawan, Jyotsna

PY - 2018/7

Y1 - 2018/7

N2 - Several studies have suggested that bone marrow stromal steam cells (BMSC) exist in a quiescent state (G0) within the in vivo niche; however, an explicit analysis of the biology of G0 state-BMSC has not been reported. We hypothesized that induction of G0 in BMSC might enhance their stem cell properties. Thus, we induced quiescence in BMSC in vitro by (a) suspension culture in a viscous medium or (b) culture on soft polyacrylamide substrate; and examined their molecular and functional phenotype. Induction of G0 was confirmed by bromo-deoxyuridine (BrdU) labelling and analysis of cell cycle gene expression. Upon reactivation and re-entry into cell cycle, G0 state-BMSC exhibited enhanced clonogenic self-renewal, preferential differentiation into osteoblastic rather than adipocytic cells and increased ectopic bone formation when implanted subcutaneously in vivo in immune-deficient mice, compared to asynchronous proliferating (pre-G0) BMSC. Global gene expression profiling revealed reprogramming of the transcriptome during G0 state including significant alterations in relevant pathways and expression of secreted factors, suggesting altered autocrine and paracrine signaling by G0 state-BMSC and a possible mechanism for enhanced bone formation. G0 state-BMSC might provide a clinically relevant model for understanding the in vivo biology of BMSC.

AB - Several studies have suggested that bone marrow stromal steam cells (BMSC) exist in a quiescent state (G0) within the in vivo niche; however, an explicit analysis of the biology of G0 state-BMSC has not been reported. We hypothesized that induction of G0 in BMSC might enhance their stem cell properties. Thus, we induced quiescence in BMSC in vitro by (a) suspension culture in a viscous medium or (b) culture on soft polyacrylamide substrate; and examined their molecular and functional phenotype. Induction of G0 was confirmed by bromo-deoxyuridine (BrdU) labelling and analysis of cell cycle gene expression. Upon reactivation and re-entry into cell cycle, G0 state-BMSC exhibited enhanced clonogenic self-renewal, preferential differentiation into osteoblastic rather than adipocytic cells and increased ectopic bone formation when implanted subcutaneously in vivo in immune-deficient mice, compared to asynchronous proliferating (pre-G0) BMSC. Global gene expression profiling revealed reprogramming of the transcriptome during G0 state including significant alterations in relevant pathways and expression of secreted factors, suggesting altered autocrine and paracrine signaling by G0 state-BMSC and a possible mechanism for enhanced bone formation. G0 state-BMSC might provide a clinically relevant model for understanding the in vivo biology of BMSC.

KW - Adhesion

KW - BMSC

KW - Cell cycle

KW - Osteoblastic differentiation

KW - Quiescence (G0)

KW - Reprogramming

KW - Substrate stiffness

KW - Suspension culture

KW - Transcriptome

KW - Cell Proliferation

KW - Humans

KW - Mesenchymal Stem Cells

KW - Animals

KW - Bone Marrow Cells/cytology

KW - Cell Differentiation

KW - Mice

KW - Stem Cells/cytology

U2 - 10.1016/j.scr.2018.05.010

DO - 10.1016/j.scr.2018.05.010

M3 - Journal article

C2 - 29803144

AN - SCOPUS:85047308513

VL - 30

SP - 69

EP - 80

JO - Stem Cell Research

JF - Stem Cell Research

SN - 1873-5061

ER -