Increased RANKL/OPG mRNA Ratio in Iliac Bone Biopsies From Women with Hip Fractures.

Basem Abdallah, L.S. Stilgren, Nis Nissen, Moustapha Kassem, H.R. Jørgensen, B. Abrahamsen

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Udgivelsesdato: 18.11.04
OriginalsprogEngelsk
TidsskriftCalcified Tissue International
Vol/bind76
Udgave nummer2
Sider (fra-til)90-97
Antal sider8
ISSN0171-967X
DOI
StatusUdgivet - 2005

Fingeraftryk

Osteoprotegerin
Messenger RNA
Bone Density
Actins
Interleukin-6
Interleukin-7
Osteogenesis
Hip
Real-Time Polymerase Chain Reaction
Tumor Necrosis Factor-alpha
Logistic Models
RNA

Bibliografisk note

Epub

Citer dette

Abdallah, Basem ; Stilgren, L.S. ; Nissen, Nis ; Kassem, Moustapha ; Jørgensen, H.R. ; Abrahamsen, B. / Increased RANKL/OPG mRNA Ratio in Iliac Bone Biopsies From Women with Hip Fractures. I: Calcified Tissue International. 2005 ; Bind 76, Nr. 2. s. 90-97.
@article{99fb4830de9f11db9628000ea68e967b,
title = "Increased RANKL/OPG mRNA Ratio in Iliac Bone Biopsies From Women with Hip Fractures.",
abstract = "RANKL (receptor activator of NF-κB) is a potent physiological inducer of osteoclastogenesis. Its actions are blocked by the decoy receptor osteoprotegerin (OPG), and treatment with OPG blocks bone resorption in postmenopausal women. Both positive and negative associations between serum OPG and bone mineral density (BMD) have been reported in the literature. We hypothesized that decreased OPG production relative to RANKL within bone itself could lead to increased risk of osteoporotic fracture. We included ten women with hip fracture (age 76.3 ± 8.0 years, N.S, hip BMD 0.686 ± 1.3 g/cm2, P < 0.05) and 24 women with osteoarthrosis of the hip (age 72.8 ± 7.2 years, hip BMD 0.832 ± 1.1 g/cm2). Transiliac biopsies were obtained at the time of surgery. Total RNA was extracted from biopsies and reverse-transcribed. Real-time quantification of mRNA was performed with a SYBR Green I real time PCR assay, calculating relative gene expession with normalization of results for β actin mRNA. Actin normalized mRNA levels for OPG and interleukin (IL)-6 were significantly lower in fracture patients, with a significantly higher RANKL/OPG ratio in patients with fractures. There was no significant difference in tumor necrosis factor (TNF), IL-1, IL-1ra, or IL-7 expression. IL-6 mRNA levels were lower in fracture patients (P < 0.05). The effect of increased RANKL/OPG ratio (Z = 2.08, P < 0.05) on fracture risk was additive to that of hip BMD T score (Z = −1.95, P < 0.05) when assessed using logistic regression. Elderly women with hip fractures exhibit an increased RANKL/OPG mRNA content of iliac bone. This is associated with increased fracture susceptibility, which is not in itself explained by low BMD.",
author = "Basem Abdallah and L.S. Stilgren and Nis Nissen and Moustapha Kassem and H.R. J{\o}rgensen and B. Abrahamsen",
note = "Epub",
year = "2005",
doi = "10.1007/s00223-004-0074-4",
language = "English",
volume = "76",
pages = "90--97",
journal = "Calcified Tissue International",
issn = "0171-967X",
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Increased RANKL/OPG mRNA Ratio in Iliac Bone Biopsies From Women with Hip Fractures. / Abdallah, Basem; Stilgren, L.S.; Nissen, Nis; Kassem, Moustapha; Jørgensen, H.R.; Abrahamsen, B.

I: Calcified Tissue International, Bind 76, Nr. 2, 2005, s. 90-97.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Increased RANKL/OPG mRNA Ratio in Iliac Bone Biopsies From Women with Hip Fractures.

AU - Abdallah, Basem

AU - Stilgren, L.S.

AU - Nissen, Nis

AU - Kassem, Moustapha

AU - Jørgensen, H.R.

AU - Abrahamsen, B.

N1 - Epub

PY - 2005

Y1 - 2005

N2 - RANKL (receptor activator of NF-κB) is a potent physiological inducer of osteoclastogenesis. Its actions are blocked by the decoy receptor osteoprotegerin (OPG), and treatment with OPG blocks bone resorption in postmenopausal women. Both positive and negative associations between serum OPG and bone mineral density (BMD) have been reported in the literature. We hypothesized that decreased OPG production relative to RANKL within bone itself could lead to increased risk of osteoporotic fracture. We included ten women with hip fracture (age 76.3 ± 8.0 years, N.S, hip BMD 0.686 ± 1.3 g/cm2, P < 0.05) and 24 women with osteoarthrosis of the hip (age 72.8 ± 7.2 years, hip BMD 0.832 ± 1.1 g/cm2). Transiliac biopsies were obtained at the time of surgery. Total RNA was extracted from biopsies and reverse-transcribed. Real-time quantification of mRNA was performed with a SYBR Green I real time PCR assay, calculating relative gene expession with normalization of results for β actin mRNA. Actin normalized mRNA levels for OPG and interleukin (IL)-6 were significantly lower in fracture patients, with a significantly higher RANKL/OPG ratio in patients with fractures. There was no significant difference in tumor necrosis factor (TNF), IL-1, IL-1ra, or IL-7 expression. IL-6 mRNA levels were lower in fracture patients (P < 0.05). The effect of increased RANKL/OPG ratio (Z = 2.08, P < 0.05) on fracture risk was additive to that of hip BMD T score (Z = −1.95, P < 0.05) when assessed using logistic regression. Elderly women with hip fractures exhibit an increased RANKL/OPG mRNA content of iliac bone. This is associated with increased fracture susceptibility, which is not in itself explained by low BMD.

AB - RANKL (receptor activator of NF-κB) is a potent physiological inducer of osteoclastogenesis. Its actions are blocked by the decoy receptor osteoprotegerin (OPG), and treatment with OPG blocks bone resorption in postmenopausal women. Both positive and negative associations between serum OPG and bone mineral density (BMD) have been reported in the literature. We hypothesized that decreased OPG production relative to RANKL within bone itself could lead to increased risk of osteoporotic fracture. We included ten women with hip fracture (age 76.3 ± 8.0 years, N.S, hip BMD 0.686 ± 1.3 g/cm2, P < 0.05) and 24 women with osteoarthrosis of the hip (age 72.8 ± 7.2 years, hip BMD 0.832 ± 1.1 g/cm2). Transiliac biopsies were obtained at the time of surgery. Total RNA was extracted from biopsies and reverse-transcribed. Real-time quantification of mRNA was performed with a SYBR Green I real time PCR assay, calculating relative gene expession with normalization of results for β actin mRNA. Actin normalized mRNA levels for OPG and interleukin (IL)-6 were significantly lower in fracture patients, with a significantly higher RANKL/OPG ratio in patients with fractures. There was no significant difference in tumor necrosis factor (TNF), IL-1, IL-1ra, or IL-7 expression. IL-6 mRNA levels were lower in fracture patients (P < 0.05). The effect of increased RANKL/OPG ratio (Z = 2.08, P < 0.05) on fracture risk was additive to that of hip BMD T score (Z = −1.95, P < 0.05) when assessed using logistic regression. Elderly women with hip fractures exhibit an increased RANKL/OPG mRNA content of iliac bone. This is associated with increased fracture susceptibility, which is not in itself explained by low BMD.

U2 - 10.1007/s00223-004-0074-4

DO - 10.1007/s00223-004-0074-4

M3 - Journal article

VL - 76

SP - 90

EP - 97

JO - Calcified Tissue International

JF - Calcified Tissue International

SN - 0171-967X

IS - 2

ER -