Increased levels of deleted in malignant brain tumours 1 (DMBT1) in active bacteria-related appendicitis

Elke Kaemmerer, Ursula Schneider, Christina Klaus, Patrick Plum, Andrea Reinartz, Maximilian Adolf, Marcus Renner, Tim G A M Wolfs, Boris W Kramer, Norbert Wagner, Jan Mollenhauer, Nikolaus Gassler

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

    Resumé

    Kaemmerer E, Schneider U, Klaus C, Plum P, Reinartz A, Adolf M, Renner M, Wolfs T G A M, Kramer B W, Wagner N, Mollenhauer J & Gassler N (2012) Histopathology Increased levels of deleted in malignant brain tumours 1 (DMBT1) in active bacteria-related appendicitis Aims:  Deleted in malignant brain tumours 1 (DMBT1; gp340) is a secreted glycoprotein which is found in the surface lining epithelia of human small and large intestine. DMBT1 is suggested to play a role in enterocyte differentiation and surface protection from intestinal bacteria. The aim of this study was to elucidate DMBT1 expression in bacteria-related active intestinal inflammation such as appendicitis. Methods and results:  mRNA and protein levels of DMBT1 were analysed in surgical resections of 50 appendices (active inflammation: n = 25). In non-actively inflamed appendices, inter-individual differences in basal DMBT1 levels of enterocytes and some non-epithelial cells were found. In active appendicitis, enterocytic DMBT1 mRNA expression was increased approximately fivefold, which was paralleled by a corresponding increase of cytoplasmic and secreted DMBT1 protein levels. Increased DMBT1 expression was predominant in enterocytes adjacent to erosive lesions or ulcers. Conclusions:  Our data demonstrate that bacteria-related active inflammation results in a sharp increase of DMBT1 levels in enterocytes. These findings substantiate the view that DMBT1 is of functional relevance for host defence and modulation of the course of intestinal bacteria-related inflammatory responses.
    OriginalsprogEngelsk
    TidsskriftHistopathology
    Vol/bind60
    Udgave nummer4
    Sider (fra-til)561-569
    ISSN0309-0167
    DOI
    StatusUdgivet - 2012

    Fingeraftryk

    Enterocytes
    Messenger RNA
    Large Intestine
    Individuality
    Small Intestine
    Ulcer
    Proteins
    Epithelium

    Citer dette

    Kaemmerer, E., Schneider, U., Klaus, C., Plum, P., Reinartz, A., Adolf, M., ... Gassler, N. (2012). Increased levels of deleted in malignant brain tumours 1 (DMBT1) in active bacteria-related appendicitis. Histopathology, 60(4), 561-569. https://doi.org/10.1111/j.1365-2559.2011.04159.x
    Kaemmerer, Elke ; Schneider, Ursula ; Klaus, Christina ; Plum, Patrick ; Reinartz, Andrea ; Adolf, Maximilian ; Renner, Marcus ; Wolfs, Tim G A M ; Kramer, Boris W ; Wagner, Norbert ; Mollenhauer, Jan ; Gassler, Nikolaus. / Increased levels of deleted in malignant brain tumours 1 (DMBT1) in active bacteria-related appendicitis. I: Histopathology. 2012 ; Bind 60, Nr. 4. s. 561-569.
    @article{4e525eb4ca9b4c40bf0506de87171ae2,
    title = "Increased levels of deleted in malignant brain tumours 1 (DMBT1) in active bacteria-related appendicitis",
    abstract = "Kaemmerer E, Schneider U, Klaus C, Plum P, Reinartz A, Adolf M, Renner M, Wolfs T G A M, Kramer B W, Wagner N, Mollenhauer J & Gassler N (2012) Histopathology Increased levels of deleted in malignant brain tumours 1 (DMBT1) in active bacteria-related appendicitis Aims:  Deleted in malignant brain tumours 1 (DMBT1; gp340) is a secreted glycoprotein which is found in the surface lining epithelia of human small and large intestine. DMBT1 is suggested to play a role in enterocyte differentiation and surface protection from intestinal bacteria. The aim of this study was to elucidate DMBT1 expression in bacteria-related active intestinal inflammation such as appendicitis. Methods and results:  mRNA and protein levels of DMBT1 were analysed in surgical resections of 50 appendices (active inflammation: n = 25). In non-actively inflamed appendices, inter-individual differences in basal DMBT1 levels of enterocytes and some non-epithelial cells were found. In active appendicitis, enterocytic DMBT1 mRNA expression was increased approximately fivefold, which was paralleled by a corresponding increase of cytoplasmic and secreted DMBT1 protein levels. Increased DMBT1 expression was predominant in enterocytes adjacent to erosive lesions or ulcers. Conclusions:  Our data demonstrate that bacteria-related active inflammation results in a sharp increase of DMBT1 levels in enterocytes. These findings substantiate the view that DMBT1 is of functional relevance for host defence and modulation of the course of intestinal bacteria-related inflammatory responses.",
    author = "Elke Kaemmerer and Ursula Schneider and Christina Klaus and Patrick Plum and Andrea Reinartz and Maximilian Adolf and Marcus Renner and Wolfs, {Tim G A M} and Kramer, {Boris W} and Norbert Wagner and Jan Mollenhauer and Nikolaus Gassler",
    note = "{\circledC} 2012 Blackwell Publishing Ltd.",
    year = "2012",
    doi = "10.1111/j.1365-2559.2011.04159.x",
    language = "English",
    volume = "60",
    pages = "561--569",
    journal = "Histopathology",
    issn = "0309-0167",
    publisher = "Wiley-Blackwell",
    number = "4",

    }

    Kaemmerer, E, Schneider, U, Klaus, C, Plum, P, Reinartz, A, Adolf, M, Renner, M, Wolfs, TGAM, Kramer, BW, Wagner, N, Mollenhauer, J & Gassler, N 2012, 'Increased levels of deleted in malignant brain tumours 1 (DMBT1) in active bacteria-related appendicitis', Histopathology, bind 60, nr. 4, s. 561-569. https://doi.org/10.1111/j.1365-2559.2011.04159.x

    Increased levels of deleted in malignant brain tumours 1 (DMBT1) in active bacteria-related appendicitis. / Kaemmerer, Elke; Schneider, Ursula; Klaus, Christina; Plum, Patrick; Reinartz, Andrea; Adolf, Maximilian; Renner, Marcus; Wolfs, Tim G A M; Kramer, Boris W; Wagner, Norbert; Mollenhauer, Jan; Gassler, Nikolaus.

    I: Histopathology, Bind 60, Nr. 4, 2012, s. 561-569.

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

    TY - JOUR

    T1 - Increased levels of deleted in malignant brain tumours 1 (DMBT1) in active bacteria-related appendicitis

    AU - Kaemmerer, Elke

    AU - Schneider, Ursula

    AU - Klaus, Christina

    AU - Plum, Patrick

    AU - Reinartz, Andrea

    AU - Adolf, Maximilian

    AU - Renner, Marcus

    AU - Wolfs, Tim G A M

    AU - Kramer, Boris W

    AU - Wagner, Norbert

    AU - Mollenhauer, Jan

    AU - Gassler, Nikolaus

    N1 - © 2012 Blackwell Publishing Ltd.

    PY - 2012

    Y1 - 2012

    N2 - Kaemmerer E, Schneider U, Klaus C, Plum P, Reinartz A, Adolf M, Renner M, Wolfs T G A M, Kramer B W, Wagner N, Mollenhauer J & Gassler N (2012) Histopathology Increased levels of deleted in malignant brain tumours 1 (DMBT1) in active bacteria-related appendicitis Aims:  Deleted in malignant brain tumours 1 (DMBT1; gp340) is a secreted glycoprotein which is found in the surface lining epithelia of human small and large intestine. DMBT1 is suggested to play a role in enterocyte differentiation and surface protection from intestinal bacteria. The aim of this study was to elucidate DMBT1 expression in bacteria-related active intestinal inflammation such as appendicitis. Methods and results:  mRNA and protein levels of DMBT1 were analysed in surgical resections of 50 appendices (active inflammation: n = 25). In non-actively inflamed appendices, inter-individual differences in basal DMBT1 levels of enterocytes and some non-epithelial cells were found. In active appendicitis, enterocytic DMBT1 mRNA expression was increased approximately fivefold, which was paralleled by a corresponding increase of cytoplasmic and secreted DMBT1 protein levels. Increased DMBT1 expression was predominant in enterocytes adjacent to erosive lesions or ulcers. Conclusions:  Our data demonstrate that bacteria-related active inflammation results in a sharp increase of DMBT1 levels in enterocytes. These findings substantiate the view that DMBT1 is of functional relevance for host defence and modulation of the course of intestinal bacteria-related inflammatory responses.

    AB - Kaemmerer E, Schneider U, Klaus C, Plum P, Reinartz A, Adolf M, Renner M, Wolfs T G A M, Kramer B W, Wagner N, Mollenhauer J & Gassler N (2012) Histopathology Increased levels of deleted in malignant brain tumours 1 (DMBT1) in active bacteria-related appendicitis Aims:  Deleted in malignant brain tumours 1 (DMBT1; gp340) is a secreted glycoprotein which is found in the surface lining epithelia of human small and large intestine. DMBT1 is suggested to play a role in enterocyte differentiation and surface protection from intestinal bacteria. The aim of this study was to elucidate DMBT1 expression in bacteria-related active intestinal inflammation such as appendicitis. Methods and results:  mRNA and protein levels of DMBT1 were analysed in surgical resections of 50 appendices (active inflammation: n = 25). In non-actively inflamed appendices, inter-individual differences in basal DMBT1 levels of enterocytes and some non-epithelial cells were found. In active appendicitis, enterocytic DMBT1 mRNA expression was increased approximately fivefold, which was paralleled by a corresponding increase of cytoplasmic and secreted DMBT1 protein levels. Increased DMBT1 expression was predominant in enterocytes adjacent to erosive lesions or ulcers. Conclusions:  Our data demonstrate that bacteria-related active inflammation results in a sharp increase of DMBT1 levels in enterocytes. These findings substantiate the view that DMBT1 is of functional relevance for host defence and modulation of the course of intestinal bacteria-related inflammatory responses.

    U2 - 10.1111/j.1365-2559.2011.04159.x

    DO - 10.1111/j.1365-2559.2011.04159.x

    M3 - Journal article

    VL - 60

    SP - 561

    EP - 569

    JO - Histopathology

    JF - Histopathology

    SN - 0309-0167

    IS - 4

    ER -