Incorporation of (R)- and (S)-3´,4´-seco-thymidine into oligodeoxynucleotides: hybridization properties and enzymatic stability

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Abstrakt

Novel flexible ollgodeoxynucleotide analogues containing (R)- and (S)-3′,4′-seco-thymidine were synthesized on an automated DNA-synthesizer using the phosphoramidlte approach. Oligodeoxynucleotide analogues (17-mers) having one or three modifications In the middle or one or two modifications In the ends were evaluated with respect to hybridization properties and enzymatic stability. 3′-End-modlfled oligomers were stable towards 3′-exonuclease degradation and displayed acceptable hybridization properties.

OriginalsprogEngelsk
TidsskriftNucleic Acids Research
Vol/bind22
Udgave nummer5
Sider (fra-til)703-710
ISSN0305-1048
DOI
StatusUdgivet - 1994

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