In vivo and in vitro translation of the RNAs of four tobamoviruses

H Beier, K W Mundry, O G Issinger

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Udgivelsesdato: 1980-null
OriginalsprogEngelsk
TidsskriftIntervirology
Vol/bind14
Udgave nummer5-6
Sider (fra-til)292-299
Antal sider7
ISSN0300-5526
StatusUdgivet - 1980

Fingeraftryk

Tobamovirus
RNA
Proteins
Molecular Weight
Viruses
Peptides
Cell-Free System
Protoplasts
Reticulocytes
Viral RNA
Capsid Proteins
In Vitro Techniques

Citer dette

Beier, H ; Mundry, K W ; Issinger, O G. / In vivo and in vitro translation of the RNAs of four tobamoviruses. I: Intervirology. 1980 ; Bind 14, Nr. 5-6. s. 292-299.
@article{b20a6d305a4311de839d000ea68e967b,
title = "In vivo and in vitro translation of the RNAs of four tobamoviruses",
abstract = "The RNAs from four tobamoviruses [tobacco mosaic virus(TMV) vulgare, TMV dahlemense, TMV U2, and the cowpea strain of TMV (CcTMV)] were translated in a cell-free ribosome system from reticulocytes. Among the translation products found were two polypeptides with molecular weights of 170,000 and 120,000 which were produced by all the viral RNAs tested. These two polypeptides comigrated with the corresponding virus-specific proteins synthesized in tobacco protoplasts infected with any of the four tobamoviruses. A 30,000 molecular weight protein was encoded by all four RNAs. However, this protein was synthesized in different amounts and differed slightly in size for each virus. When TMV vulgare or dahlemense RNAs were used as templates, this protein was one of the major translation products, whereas trace amounts were produced in the presence of U2 or CcTMV RNA. Only the CcTMV RNA directed the in vitro synthesis of a protein which had about the same electrophoretic mobility as coat protein.",
keywords = "Molecular Weight, Plants, Toxic, Protein Biosynthesis, Protoplasts, RNA, Viral, Tobacco, Tobacco Mosaic Virus, Viral Proteins",
author = "H Beier and Mundry, {K W} and Issinger, {O G}",
year = "1980",
language = "English",
volume = "14",
pages = "292--299",
journal = "Intervirology",
issn = "0300-5526",
publisher = "S. Karger AG",
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In vivo and in vitro translation of the RNAs of four tobamoviruses. / Beier, H; Mundry, K W; Issinger, O G.

I: Intervirology, Bind 14, Nr. 5-6, 1980, s. 292-299.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - In vivo and in vitro translation of the RNAs of four tobamoviruses

AU - Beier, H

AU - Mundry, K W

AU - Issinger, O G

PY - 1980

Y1 - 1980

N2 - The RNAs from four tobamoviruses [tobacco mosaic virus(TMV) vulgare, TMV dahlemense, TMV U2, and the cowpea strain of TMV (CcTMV)] were translated in a cell-free ribosome system from reticulocytes. Among the translation products found were two polypeptides with molecular weights of 170,000 and 120,000 which were produced by all the viral RNAs tested. These two polypeptides comigrated with the corresponding virus-specific proteins synthesized in tobacco protoplasts infected with any of the four tobamoviruses. A 30,000 molecular weight protein was encoded by all four RNAs. However, this protein was synthesized in different amounts and differed slightly in size for each virus. When TMV vulgare or dahlemense RNAs were used as templates, this protein was one of the major translation products, whereas trace amounts were produced in the presence of U2 or CcTMV RNA. Only the CcTMV RNA directed the in vitro synthesis of a protein which had about the same electrophoretic mobility as coat protein.

AB - The RNAs from four tobamoviruses [tobacco mosaic virus(TMV) vulgare, TMV dahlemense, TMV U2, and the cowpea strain of TMV (CcTMV)] were translated in a cell-free ribosome system from reticulocytes. Among the translation products found were two polypeptides with molecular weights of 170,000 and 120,000 which were produced by all the viral RNAs tested. These two polypeptides comigrated with the corresponding virus-specific proteins synthesized in tobacco protoplasts infected with any of the four tobamoviruses. A 30,000 molecular weight protein was encoded by all four RNAs. However, this protein was synthesized in different amounts and differed slightly in size for each virus. When TMV vulgare or dahlemense RNAs were used as templates, this protein was one of the major translation products, whereas trace amounts were produced in the presence of U2 or CcTMV RNA. Only the CcTMV RNA directed the in vitro synthesis of a protein which had about the same electrophoretic mobility as coat protein.

KW - Molecular Weight

KW - Plants, Toxic

KW - Protein Biosynthesis

KW - Protoplasts

KW - RNA, Viral

KW - Tobacco

KW - Tobacco Mosaic Virus

KW - Viral Proteins

M3 - Journal article

VL - 14

SP - 292

EP - 299

JO - Intervirology

JF - Intervirology

SN - 0300-5526

IS - 5-6

ER -