In vitro cell quality of articular chondrocytes assigned for autologous implantation in dependence of specific patient characteristics

Jan M Pestka, Hagen Schmal, Gian Salzmann, Jochen Hecky, Norbert P Südkamp, Philipp Niemeyer

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

OBJECTIVE: Autologous chondrocyte implantation (ACI) is a well-established therapeutic option for the treatment of cartilage defects of the knee joint. Since information concerning the cellular aspects of ACI is still limited, the aim of the present study was to investigate relevant differences between chondrocyte quality after in vitro cultivation and possible correlations with patient-specific factors.

DESIGN: Cell quality of 252 consecutive ACI patients was assessed after chondrocyte in vitro expansion by determination of the expression of cartilage relevant surface marker CD44 and cartilage-specific differentiation markers (aggrecan and collagen type II). All cell quality parameters were correlated with patient-specific parameters, such as age, size and defect location, number of defects and grade of joint degeneration according to the Kellgren-Lawrence classification.

RESULTS: Neither the expression of CD44, aggrecan or collagen type II nor cell density or viability after proliferation seemed to correlate with the grade of joint degeneration, defect aetiology or patient gender. However, chondrocytes harvested from the knee joints of patients at less than 20 years of age showed significantly higher expression rates of cartilage-specific markers when compared to older patients' chondrocytes.

CONCLUSIONS: The present study identifies relevant differences concerning chondrocyte quality after in vitro expansion in a highly preselected study population of 252 patients that from a surgical point of view were eligible for ACI. With the exception of patients aged 20 years or younger, no patient-specific parameters could be identified which might allow anticipation of cell quality in individual patients.

OriginalsprogEngelsk
TidsskriftArchives of Orthopaedic and Trauma Surgery
Vol/bind131
Udgave nummer6
Sider (fra-til)779-89
Antal sider11
ISSN0936-8051
DOI
StatusUdgivet - jun. 2011

Fingeraftryk

Chondrocytes
Joints
Collagen Type II
Knee Joint
In Vitro Techniques
Cell Survival

Citer dette

Pestka, Jan M ; Schmal, Hagen ; Salzmann, Gian ; Hecky, Jochen ; Südkamp, Norbert P ; Niemeyer, Philipp. / In vitro cell quality of articular chondrocytes assigned for autologous implantation in dependence of specific patient characteristics. I: Archives of Orthopaedic and Trauma Surgery. 2011 ; Bind 131, Nr. 6. s. 779-89.
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abstract = "OBJECTIVE: Autologous chondrocyte implantation (ACI) is a well-established therapeutic option for the treatment of cartilage defects of the knee joint. Since information concerning the cellular aspects of ACI is still limited, the aim of the present study was to investigate relevant differences between chondrocyte quality after in vitro cultivation and possible correlations with patient-specific factors.DESIGN: Cell quality of 252 consecutive ACI patients was assessed after chondrocyte in vitro expansion by determination of the expression of cartilage relevant surface marker CD44 and cartilage-specific differentiation markers (aggrecan and collagen type II). All cell quality parameters were correlated with patient-specific parameters, such as age, size and defect location, number of defects and grade of joint degeneration according to the Kellgren-Lawrence classification.RESULTS: Neither the expression of CD44, aggrecan or collagen type II nor cell density or viability after proliferation seemed to correlate with the grade of joint degeneration, defect aetiology or patient gender. However, chondrocytes harvested from the knee joints of patients at less than 20 years of age showed significantly higher expression rates of cartilage-specific markers when compared to older patients' chondrocytes.CONCLUSIONS: The present study identifies relevant differences concerning chondrocyte quality after in vitro expansion in a highly preselected study population of 252 patients that from a surgical point of view were eligible for ACI. With the exception of patients aged 20 years or younger, no patient-specific parameters could be identified which might allow anticipation of cell quality in individual patients.",
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In vitro cell quality of articular chondrocytes assigned for autologous implantation in dependence of specific patient characteristics. / Pestka, Jan M; Schmal, Hagen; Salzmann, Gian; Hecky, Jochen; Südkamp, Norbert P; Niemeyer, Philipp.

I: Archives of Orthopaedic and Trauma Surgery, Bind 131, Nr. 6, 06.2011, s. 779-89.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - In vitro cell quality of articular chondrocytes assigned for autologous implantation in dependence of specific patient characteristics

AU - Pestka, Jan M

AU - Schmal, Hagen

AU - Salzmann, Gian

AU - Hecky, Jochen

AU - Südkamp, Norbert P

AU - Niemeyer, Philipp

PY - 2011/6

Y1 - 2011/6

N2 - OBJECTIVE: Autologous chondrocyte implantation (ACI) is a well-established therapeutic option for the treatment of cartilage defects of the knee joint. Since information concerning the cellular aspects of ACI is still limited, the aim of the present study was to investigate relevant differences between chondrocyte quality after in vitro cultivation and possible correlations with patient-specific factors.DESIGN: Cell quality of 252 consecutive ACI patients was assessed after chondrocyte in vitro expansion by determination of the expression of cartilage relevant surface marker CD44 and cartilage-specific differentiation markers (aggrecan and collagen type II). All cell quality parameters were correlated with patient-specific parameters, such as age, size and defect location, number of defects and grade of joint degeneration according to the Kellgren-Lawrence classification.RESULTS: Neither the expression of CD44, aggrecan or collagen type II nor cell density or viability after proliferation seemed to correlate with the grade of joint degeneration, defect aetiology or patient gender. However, chondrocytes harvested from the knee joints of patients at less than 20 years of age showed significantly higher expression rates of cartilage-specific markers when compared to older patients' chondrocytes.CONCLUSIONS: The present study identifies relevant differences concerning chondrocyte quality after in vitro expansion in a highly preselected study population of 252 patients that from a surgical point of view were eligible for ACI. With the exception of patients aged 20 years or younger, no patient-specific parameters could be identified which might allow anticipation of cell quality in individual patients.

AB - OBJECTIVE: Autologous chondrocyte implantation (ACI) is a well-established therapeutic option for the treatment of cartilage defects of the knee joint. Since information concerning the cellular aspects of ACI is still limited, the aim of the present study was to investigate relevant differences between chondrocyte quality after in vitro cultivation and possible correlations with patient-specific factors.DESIGN: Cell quality of 252 consecutive ACI patients was assessed after chondrocyte in vitro expansion by determination of the expression of cartilage relevant surface marker CD44 and cartilage-specific differentiation markers (aggrecan and collagen type II). All cell quality parameters were correlated with patient-specific parameters, such as age, size and defect location, number of defects and grade of joint degeneration according to the Kellgren-Lawrence classification.RESULTS: Neither the expression of CD44, aggrecan or collagen type II nor cell density or viability after proliferation seemed to correlate with the grade of joint degeneration, defect aetiology or patient gender. However, chondrocytes harvested from the knee joints of patients at less than 20 years of age showed significantly higher expression rates of cartilage-specific markers when compared to older patients' chondrocytes.CONCLUSIONS: The present study identifies relevant differences concerning chondrocyte quality after in vitro expansion in a highly preselected study population of 252 patients that from a surgical point of view were eligible for ACI. With the exception of patients aged 20 years or younger, no patient-specific parameters could be identified which might allow anticipation of cell quality in individual patients.

KW - Adult

KW - Age Factors

KW - Aggrecans

KW - Antigens, CD44

KW - Biomarkers

KW - Biopsy

KW - Cartilage, Articular

KW - Cell Count

KW - Cell Differentiation

KW - Cell Survival

KW - Chondrocytes

KW - Collagen Type II

KW - Female

KW - Humans

KW - In Vitro Techniques

KW - Knee Joint

KW - Male

KW - Middle Aged

KW - Transplantation, Autologous

KW - Young Adult

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1007/s00402-010-1219-8

DO - 10.1007/s00402-010-1219-8

M3 - Journal article

C2 - 21165635

VL - 131

SP - 779

EP - 789

JO - Archives of Orthopaedic and Trauma Surgery

JF - Archives of Orthopaedic and Trauma Surgery

SN - 0936-8051

IS - 6

ER -