In vitro and in vivo developmental competence of ovulated and in vitro matured porcine oocytes activated by electrical activation

Jie Zhu, Tim King, John Dobrinsky, Linda Harkness, Tricia Ferrier, Wim Bosma, Lori L Schreier, H David Guthrie, Paul DeSousa, Ian Wilmut

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Udgivelsesdato: 2003-null
OriginalsprogEngelsk
TidsskriftCloning and Stem Cells
Vol/bind5
Udgave nummer4
Sider (fra-til)355-65
Antal sider10
ISSN1536-2302
DOI
StatusUdgivet - 1. jan. 2003
Udgivet eksterntJa

Fingeraftryk

Mental Competency
Oocytes
In Vitro Techniques
Fetus
Embryonic Structures

Citer dette

Zhu, Jie ; King, Tim ; Dobrinsky, John ; Harkness, Linda ; Ferrier, Tricia ; Bosma, Wim ; Schreier, Lori L ; Guthrie, H David ; DeSousa, Paul ; Wilmut, Ian. / In vitro and in vivo developmental competence of ovulated and in vitro matured porcine oocytes activated by electrical activation. I: Cloning and Stem Cells. 2003 ; Bind 5, Nr. 4. s. 355-65.
@article{ffc022f0e30011dd990c000ea68e967b,
title = "In vitro and in vivo developmental competence of ovulated and in vitro matured porcine oocytes activated by electrical activation",
abstract = "The objective of this study was to evaluate the in vitro and in vivo developmental competence of parthenogenetic (parthenote) pig embryos derived from ovulated and in vitro matured (IVM) oocytes. A total of four experiments were carried out. These demonstrated that the mean blastocyst rates from stimulated ovulated and IVM pig oocytes were not significantly different (61{\%} vs. 46{\%}, p > 0.05) following in vitro culture. Both ovulated and IVM pig parthenotes were able to develop in vivo for 30 days. Parthenote fetuses collected 21 and 30 days post estrus were morphologically normal but significantly smaller and lighter than fertilized controls (p < 0.01). IVM pig parthenotes stopped development around 31 days post estrus.",
author = "Jie Zhu and Tim King and John Dobrinsky and Linda Harkness and Tricia Ferrier and Wim Bosma and Schreier, {Lori L} and Guthrie, {H David} and Paul DeSousa and Ian Wilmut",
year = "2003",
month = "1",
day = "1",
doi = "10.1089/153623003772032853",
language = "English",
volume = "5",
pages = "355--65",
journal = "Cellular Reprogramming",
issn = "2152-4971",
publisher = "Mary Ann Liebert Inc. Publishers",
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Zhu, J, King, T, Dobrinsky, J, Harkness, L, Ferrier, T, Bosma, W, Schreier, LL, Guthrie, HD, DeSousa, P & Wilmut, I 2003, 'In vitro and in vivo developmental competence of ovulated and in vitro matured porcine oocytes activated by electrical activation', Cloning and Stem Cells, bind 5, nr. 4, s. 355-65. https://doi.org/10.1089/153623003772032853

In vitro and in vivo developmental competence of ovulated and in vitro matured porcine oocytes activated by electrical activation. / Zhu, Jie; King, Tim; Dobrinsky, John; Harkness, Linda; Ferrier, Tricia; Bosma, Wim; Schreier, Lori L; Guthrie, H David; DeSousa, Paul; Wilmut, Ian.

I: Cloning and Stem Cells, Bind 5, Nr. 4, 01.01.2003, s. 355-65.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - In vitro and in vivo developmental competence of ovulated and in vitro matured porcine oocytes activated by electrical activation

AU - Zhu, Jie

AU - King, Tim

AU - Dobrinsky, John

AU - Harkness, Linda

AU - Ferrier, Tricia

AU - Bosma, Wim

AU - Schreier, Lori L

AU - Guthrie, H David

AU - DeSousa, Paul

AU - Wilmut, Ian

PY - 2003/1/1

Y1 - 2003/1/1

N2 - The objective of this study was to evaluate the in vitro and in vivo developmental competence of parthenogenetic (parthenote) pig embryos derived from ovulated and in vitro matured (IVM) oocytes. A total of four experiments were carried out. These demonstrated that the mean blastocyst rates from stimulated ovulated and IVM pig oocytes were not significantly different (61% vs. 46%, p > 0.05) following in vitro culture. Both ovulated and IVM pig parthenotes were able to develop in vivo for 30 days. Parthenote fetuses collected 21 and 30 days post estrus were morphologically normal but significantly smaller and lighter than fertilized controls (p < 0.01). IVM pig parthenotes stopped development around 31 days post estrus.

AB - The objective of this study was to evaluate the in vitro and in vivo developmental competence of parthenogenetic (parthenote) pig embryos derived from ovulated and in vitro matured (IVM) oocytes. A total of four experiments were carried out. These demonstrated that the mean blastocyst rates from stimulated ovulated and IVM pig oocytes were not significantly different (61% vs. 46%, p > 0.05) following in vitro culture. Both ovulated and IVM pig parthenotes were able to develop in vivo for 30 days. Parthenote fetuses collected 21 and 30 days post estrus were morphologically normal but significantly smaller and lighter than fertilized controls (p < 0.01). IVM pig parthenotes stopped development around 31 days post estrus.

U2 - 10.1089/153623003772032853

DO - 10.1089/153623003772032853

M3 - Journal article

VL - 5

SP - 355

EP - 365

JO - Cellular Reprogramming

JF - Cellular Reprogramming

SN - 2152-4971

IS - 4

ER -