Improving Fucoidan Yield from Fucus Brown Algae by Microwave Extraction

Signe Helle Ptak*, Knud Villy Christensen, Rafael Meichßner, Xavier Fretté

*Kontaktforfatter for dette arbejde

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Resumé

The bioactive sulfated polysaccharide from brown algae, fucoidan, can be used for a wide array of applications. As with other natural products, there are seasonal variabilities as well as variability within the investigated species, across regions, and from using different extraction procedures. In this study, the use of hot demineralized water and two variations of hot acidified water (10 mM Sulfuric acid and 100 mM hydrochloric acid) as extraction solvents for microwave extraction of fucoidan from three different brown algae of the Fucus genus (F. vesiculosus, F. serratus, and F. evanescens) were investigated. The effect on yield, degree of sulfation, and the molecular weight of the fucoidan from the different solvents at temperatures 80 °C, 100 °C, 120 °C, were tested. The Fucus used in this study were harvested in the Baltic Sea in the Kiel Fjord, Germany, during Summer and Autumn of 2017. Air dried F. vesiculosus from Brittany in France was also analyzed and used for optimization of the extraction method and as a reference sample. The extraction procedure was adapted and modified from the method provided by Fletcher et al, 2017. The extracts were purified by performing dialysis. The results showed that fucoidan yield is maximized by extracting with 10 mM sulfuric acid for all species investigated. A large seasonal variance between species was observed, and large differences in yield were also dependent on species. These results suggest that to maximize fucoidan yield, one should tailor the extraction method to the specific algae species used, however, MAE with 10 mM sulfuric acid proves a good general extraction method.
OriginalsprogEngelsk
BogserieChemical engineering transactions
Vol/bind74
Sider (fra-til)109-114
ISSN1974-9791
DOI
StatusUdgivet - 31. maj 2019
Begivenhed14th International Conference on Chemical and Process Engineering - RoyalCarlton Hotel, Via Montebello 8, Bologna, Italien
Varighed: 26. maj 201929. maj 2019
Konferencens nummer: 14
http://www.aidic.it/icheap14/

Konference

Konference14th International Conference on Chemical and Process Engineering
Nummer14
LokationRoyalCarlton Hotel, Via Montebello 8
LandItalien
ByBologna
Periode26/05/201929/05/2019
Internetadresse

Fingeraftryk

Algae
Microwaves
Sulfuric acid
Dialysis
Hydrochloric Acid
Water
Solvent extraction
Hydrochloric acid
Polysaccharides
Biological Products
fucoidan
Molecular weight
Air
sulfuric acid

Citer dette

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title = "Improving Fucoidan Yield from Fucus Brown Algae by Microwave Extraction",
abstract = "The bioactive sulfated polysaccharide from brown algae, fucoidan, can be used for a wide array of applications. As with other natural products, there are seasonal variabilities as well as variability within the investigated species, across regions, and from using different extraction procedures. In this study, the use of hot demineralized water and two variations of hot acidified water (10 mM Sulfuric acid and 100 mM hydrochloric acid) as extraction solvents for microwave extraction of fucoidan from three different brown algae of the Fucus genus (F. vesiculosus, F. serratus, and F. evanescens) were investigated. The effect on yield, degree of sulfation, and the molecular weight of the fucoidan from the different solvents at temperatures 80 °C, 100 °C, 120 °C, were tested. The Fucus used in this study were harvested in the Baltic Sea in the Kiel Fjord, Germany, during Summer and Autumn of 2017. Air dried F. vesiculosus from Brittany in France was also analyzed and used for optimization of the extraction method and as a reference sample. The extraction procedure was adapted and modified from the method provided by Fletcher et al, 2017. The extracts were purified by performing dialysis. The results showed that fucoidan yield is maximized by extracting with 10 mM sulfuric acid for all species investigated. A large seasonal variance between species was observed, and large differences in yield were also dependent on species. These results suggest that to maximize fucoidan yield, one should tailor the extraction method to the specific algae species used, however, MAE with 10 mM sulfuric acid proves a good general extraction method.",
author = "Ptak, {Signe Helle} and Christensen, {Knud Villy} and Rafael Meich{\ss}ner and Xavier Frett{\'e}",
year = "2019",
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Improving Fucoidan Yield from Fucus Brown Algae by Microwave Extraction. / Ptak, Signe Helle; Christensen, Knud Villy; Meichßner, Rafael; Fretté, Xavier.

I: Chemical engineering transactions, Bind 74, 31.05.2019, s. 109-114.

Publikation: Bidrag til tidsskriftKonferenceartikelForskningpeer review

TY - GEN

T1 - Improving Fucoidan Yield from Fucus Brown Algae by Microwave Extraction

AU - Ptak, Signe Helle

AU - Christensen, Knud Villy

AU - Meichßner, Rafael

AU - Fretté, Xavier

PY - 2019/5/31

Y1 - 2019/5/31

N2 - The bioactive sulfated polysaccharide from brown algae, fucoidan, can be used for a wide array of applications. As with other natural products, there are seasonal variabilities as well as variability within the investigated species, across regions, and from using different extraction procedures. In this study, the use of hot demineralized water and two variations of hot acidified water (10 mM Sulfuric acid and 100 mM hydrochloric acid) as extraction solvents for microwave extraction of fucoidan from three different brown algae of the Fucus genus (F. vesiculosus, F. serratus, and F. evanescens) were investigated. The effect on yield, degree of sulfation, and the molecular weight of the fucoidan from the different solvents at temperatures 80 °C, 100 °C, 120 °C, were tested. The Fucus used in this study were harvested in the Baltic Sea in the Kiel Fjord, Germany, during Summer and Autumn of 2017. Air dried F. vesiculosus from Brittany in France was also analyzed and used for optimization of the extraction method and as a reference sample. The extraction procedure was adapted and modified from the method provided by Fletcher et al, 2017. The extracts were purified by performing dialysis. The results showed that fucoidan yield is maximized by extracting with 10 mM sulfuric acid for all species investigated. A large seasonal variance between species was observed, and large differences in yield were also dependent on species. These results suggest that to maximize fucoidan yield, one should tailor the extraction method to the specific algae species used, however, MAE with 10 mM sulfuric acid proves a good general extraction method.

AB - The bioactive sulfated polysaccharide from brown algae, fucoidan, can be used for a wide array of applications. As with other natural products, there are seasonal variabilities as well as variability within the investigated species, across regions, and from using different extraction procedures. In this study, the use of hot demineralized water and two variations of hot acidified water (10 mM Sulfuric acid and 100 mM hydrochloric acid) as extraction solvents for microwave extraction of fucoidan from three different brown algae of the Fucus genus (F. vesiculosus, F. serratus, and F. evanescens) were investigated. The effect on yield, degree of sulfation, and the molecular weight of the fucoidan from the different solvents at temperatures 80 °C, 100 °C, 120 °C, were tested. The Fucus used in this study were harvested in the Baltic Sea in the Kiel Fjord, Germany, during Summer and Autumn of 2017. Air dried F. vesiculosus from Brittany in France was also analyzed and used for optimization of the extraction method and as a reference sample. The extraction procedure was adapted and modified from the method provided by Fletcher et al, 2017. The extracts were purified by performing dialysis. The results showed that fucoidan yield is maximized by extracting with 10 mM sulfuric acid for all species investigated. A large seasonal variance between species was observed, and large differences in yield were also dependent on species. These results suggest that to maximize fucoidan yield, one should tailor the extraction method to the specific algae species used, however, MAE with 10 mM sulfuric acid proves a good general extraction method.

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DO - 10.3303/CET1974019

M3 - Conference article

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EP - 114

JO - Chemical engineering transactions

JF - Chemical engineering transactions

SN - 1974-9791

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