Impaired hyperpolarization in regenerated endothelium after balloon catheter injury

R Köhler, S Brakemeier, M Kühn, C Behrens, R Real, C Degenhardt, H D Orzechowski, A R Pries, M Paul, J Hoyer

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Udgivelsesdato: 2001-Jul-20
OriginalsprogEngelsk
TidsskriftCirculation Research
Vol/bind89
Udgave nummer2
Sider (fra-til)174-9
Antal sider5
ISSN0009-7330
StatusUdgivet - 20. jul. 2001
Udgivet eksterntJa

Fingeraftryk

Endothelium
Catheters
Dilatation
Wounds and Injuries
Acetylcholine
Cell Membrane
Charybdotoxin
Patch-Clamp Techniques
Homeostasis
Polymerase Chain Reaction

Citer dette

Köhler, R., Brakemeier, S., Kühn, M., Behrens, C., Real, R., Degenhardt, C., ... Hoyer, J. (2001). Impaired hyperpolarization in regenerated endothelium after balloon catheter injury. Circulation Research, 89(2), 174-9.
Köhler, R ; Brakemeier, S ; Kühn, M ; Behrens, C ; Real, R ; Degenhardt, C ; Orzechowski, H D ; Pries, A R ; Paul, M ; Hoyer, J. / Impaired hyperpolarization in regenerated endothelium after balloon catheter injury. I: Circulation Research. 2001 ; Bind 89, Nr. 2. s. 174-9.
@article{a9e253a0b95e11deab49000ea68e967b,
title = "Impaired hyperpolarization in regenerated endothelium after balloon catheter injury",
abstract = "Ca(2+)-activated K(+) (K(Ca)) channels control endothelial Ca(2+) homeostasis and the formation of vasodilators. After angioplasty, dysfunction of the regenerated endothelium leads to abnormal vasoregulation. In this study, we tested the expression and function of K(Ca) channels in regenerated endothelium at 6 weeks after balloon catheter injury of rat carotid arteries (CAs) by using single-cell reverse transcription-polymerase chain reaction, patch-clamp techniques, and analysis of vasoreactivity. In single regenerated endothelial cells (ECs), the percentage of ECs expressing the K(Ca) genes, rSK3 (12+/-8{\%}) and rIK1 (22+/-9{\%}), was significantly lower compared with the percentage of native ECs expressing these genes (rSK3 58+/-8{\%}, rIK1 64+/-10{\%}). In patch-clamp experiments, K(Ca) currents and acetylcholine-induced hyperpolarization were markedly reduced in regenerated ECs (shift of membrane potential -6+/-3 mV) compared with those in native ECs (shift of membrane potential -21+/-5 mV). In pressure myograph experiments, acetylcholine-induced dilation was impaired in reendothelialized CAs compared with normal CAs. Intraluminal application of the K(Ca) blocker apamin and charybdotoxin inhibited dilation by 30{\%} in normal CAs but was without effect in reendothelialized CAs. Intraluminal application of 1-ethyl-2-benzimidazolinone (100 micromol/L), an opener of K(Ca) channels, evoked dilation by 29{\%} in normal CAs but had no effect in reendothelialized CAs. In conclusion, the impaired expression of K(Ca) channels in regenerated endothelium results in defective hyperpolarization and impaired dilation. Thus, the impaired K(Ca) channel function contributes to functional alterations of regenerated endothelium after angioplasty.",
author = "R K{\"o}hler and S Brakemeier and M K{\"u}hn and C Behrens and R Real and C Degenhardt and Orzechowski, {H D} and Pries, {A R} and M Paul and J Hoyer",
year = "2001",
month = "7",
day = "20",
language = "English",
volume = "89",
pages = "174--9",
journal = "Circulation Research",
issn = "0009-7330",
publisher = "Lippincott Williams & Wilkins",
number = "2",

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Köhler, R, Brakemeier, S, Kühn, M, Behrens, C, Real, R, Degenhardt, C, Orzechowski, HD, Pries, AR, Paul, M & Hoyer, J 2001, 'Impaired hyperpolarization in regenerated endothelium after balloon catheter injury', Circulation Research, bind 89, nr. 2, s. 174-9.

Impaired hyperpolarization in regenerated endothelium after balloon catheter injury. / Köhler, R; Brakemeier, S; Kühn, M; Behrens, C; Real, R; Degenhardt, C; Orzechowski, H D; Pries, A R; Paul, M; Hoyer, J.

I: Circulation Research, Bind 89, Nr. 2, 20.07.2001, s. 174-9.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Impaired hyperpolarization in regenerated endothelium after balloon catheter injury

AU - Köhler, R

AU - Brakemeier, S

AU - Kühn, M

AU - Behrens, C

AU - Real, R

AU - Degenhardt, C

AU - Orzechowski, H D

AU - Pries, A R

AU - Paul, M

AU - Hoyer, J

PY - 2001/7/20

Y1 - 2001/7/20

N2 - Ca(2+)-activated K(+) (K(Ca)) channels control endothelial Ca(2+) homeostasis and the formation of vasodilators. After angioplasty, dysfunction of the regenerated endothelium leads to abnormal vasoregulation. In this study, we tested the expression and function of K(Ca) channels in regenerated endothelium at 6 weeks after balloon catheter injury of rat carotid arteries (CAs) by using single-cell reverse transcription-polymerase chain reaction, patch-clamp techniques, and analysis of vasoreactivity. In single regenerated endothelial cells (ECs), the percentage of ECs expressing the K(Ca) genes, rSK3 (12+/-8%) and rIK1 (22+/-9%), was significantly lower compared with the percentage of native ECs expressing these genes (rSK3 58+/-8%, rIK1 64+/-10%). In patch-clamp experiments, K(Ca) currents and acetylcholine-induced hyperpolarization were markedly reduced in regenerated ECs (shift of membrane potential -6+/-3 mV) compared with those in native ECs (shift of membrane potential -21+/-5 mV). In pressure myograph experiments, acetylcholine-induced dilation was impaired in reendothelialized CAs compared with normal CAs. Intraluminal application of the K(Ca) blocker apamin and charybdotoxin inhibited dilation by 30% in normal CAs but was without effect in reendothelialized CAs. Intraluminal application of 1-ethyl-2-benzimidazolinone (100 micromol/L), an opener of K(Ca) channels, evoked dilation by 29% in normal CAs but had no effect in reendothelialized CAs. In conclusion, the impaired expression of K(Ca) channels in regenerated endothelium results in defective hyperpolarization and impaired dilation. Thus, the impaired K(Ca) channel function contributes to functional alterations of regenerated endothelium after angioplasty.

AB - Ca(2+)-activated K(+) (K(Ca)) channels control endothelial Ca(2+) homeostasis and the formation of vasodilators. After angioplasty, dysfunction of the regenerated endothelium leads to abnormal vasoregulation. In this study, we tested the expression and function of K(Ca) channels in regenerated endothelium at 6 weeks after balloon catheter injury of rat carotid arteries (CAs) by using single-cell reverse transcription-polymerase chain reaction, patch-clamp techniques, and analysis of vasoreactivity. In single regenerated endothelial cells (ECs), the percentage of ECs expressing the K(Ca) genes, rSK3 (12+/-8%) and rIK1 (22+/-9%), was significantly lower compared with the percentage of native ECs expressing these genes (rSK3 58+/-8%, rIK1 64+/-10%). In patch-clamp experiments, K(Ca) currents and acetylcholine-induced hyperpolarization were markedly reduced in regenerated ECs (shift of membrane potential -6+/-3 mV) compared with those in native ECs (shift of membrane potential -21+/-5 mV). In pressure myograph experiments, acetylcholine-induced dilation was impaired in reendothelialized CAs compared with normal CAs. Intraluminal application of the K(Ca) blocker apamin and charybdotoxin inhibited dilation by 30% in normal CAs but was without effect in reendothelialized CAs. Intraluminal application of 1-ethyl-2-benzimidazolinone (100 micromol/L), an opener of K(Ca) channels, evoked dilation by 29% in normal CAs but had no effect in reendothelialized CAs. In conclusion, the impaired expression of K(Ca) channels in regenerated endothelium results in defective hyperpolarization and impaired dilation. Thus, the impaired K(Ca) channel function contributes to functional alterations of regenerated endothelium after angioplasty.

M3 - Journal article

C2 - 11463725

VL - 89

SP - 174

EP - 179

JO - Circulation Research

JF - Circulation Research

SN - 0009-7330

IS - 2

ER -

Köhler R, Brakemeier S, Kühn M, Behrens C, Real R, Degenhardt C et al. Impaired hyperpolarization in regenerated endothelium after balloon catheter injury. Circulation Research. 2001 jul 20;89(2):174-9.