Immunoprofiling of peripheral blood from infectious bronchitis virus vaccinated MHC-B chicken lines – Monocyte MHC-II expression as a potential correlate of protection

Frederik T. Larsen, Bertrand Bed'Hom, Mohammad Naghizadeh, Rikke B. Kjærup, Siamak Zohari, Tina S. Dalgaard*

*Kontaktforfatter for dette arbejde

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Abstrakt

Vaccination programs are implemented in poultry farms to limit outbreaks and spread of infectious bronchitis virus (IBV), which is a substantial economic burden in the poultry industry. Immune correlates, used to predict vaccine efficacy, have proved difficult to find for IBV-vaccine-induced protection. To find correlates of IBV-vaccine-induced protection, hence, we employed a flow cytometric assay to quantify peripheral leucocyte subsets and expression of cell surface markers of six different non-vaccinated and vaccinated Major Histocompatibility Complex (MHC) haplotypes. Non-vaccinated and vaccinated MHC haplotypes presented differential leucocyte composition and IBV viral load. A strong effect of MHC-B, but not vaccination, on several leucocyte subsets resulted in positive correlations with IBV viral load based on MHC haplotype ranking. In addition, a strong effect of MHC-B and vaccination on monocyte MHC-II expression showed that animals with highest monocyte MHC-II expression had weakest vaccine-induced protection. In conclusion, we found several interesting MHC-B related immune correlates of protection and that flow cytometric analysis can be employed to study correlates of IBV-vaccine-induced protection.

OriginalsprogEngelsk
TidsskriftDevelopmental and Comparative Immunology
Vol/bind96
Sider (fra-til)93-102
ISSN0145-305X
DOI
StatusUdgivet - jul. 2019
Udgivet eksterntJa

Bibliografisk note

Funding Information:
This work was supported by the Danish Council for Independent Research (grant number 6111-00304B ) and Fjerkræafgiftsfonden (grant number FAF2017010 ).

Publisher Copyright:
© 2019 Elsevier Ltd

Copyright:
Copyright 2019 Elsevier B.V., All rights reserved.

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