Immunolocalization of RANK and RANKL along the root surface and in the periodontal membrane of human primary and permanent teeth

Marie-Louise Bastholm Bille, Axel Bjarke Korsgaard Thomsen, Thomas Levin Andersen, Inger Kjær

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Abstract Objective. Root resorption, impaired tooth eruption and early tooth loss have been described in relation to diseases that involve defects in the RANK-RANKL-OPG-expression. The aim of the present immunhistochemical study was to localize and compare the reactions for RANK and membrane-bound RANKL along root surfaces and in the periodontal membrane in close proximity to the root surface of human primary and permanent teeth. Materials and methods. The material comprised extracted human teeth (11 primary teeth and six permanent teeth) from 10 different patients. Paraffin sections were prepared of each tooth and sections of each tooth were immunohistochemically stained with antibodies specific for membrane-bound RANKL and RANK. Results. The root surface and the periodontal membrane in close proximity to the root surface did not show immunoreactivity for RANKL. RANKL was only located in odontoblasts and in cells along denticles in one primary tooth. RANK was located in mononuclear cells in the pulp and in multinucleated odontoclasts along resorbed root surfaces and along resorbed dentin surfaces in the pulp in primary teeth and one permanent tooth. Conclusions. This study demonstrated RANK positivity in resorption areas in primary and permanent teeth. RANKL was positive in the pulp of one primary tooth. RANK expression in odontoclasts and RANKL expression in the pulp may indicate that RANK/RANKL play a role during resorption.
OriginalsprogEngelsk
TidsskriftActa Odontologica Scandinavica
Vol/bind70
Udgave nummer4
Sider (fra-til)265-271
ISSN0001-6357
DOI
StatusUdgivet - 2012

Fingeraftryk

Membranes
Osteoclasts
Dental Pulp Calcification
Root Resorption
Odontoblasts
Tooth Loss
Paraffin

Citer dette

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title = "Immunolocalization of RANK and RANKL along the root surface and in the periodontal membrane of human primary and permanent teeth",
abstract = "Abstract Objective. Root resorption, impaired tooth eruption and early tooth loss have been described in relation to diseases that involve defects in the RANK-RANKL-OPG-expression. The aim of the present immunhistochemical study was to localize and compare the reactions for RANK and membrane-bound RANKL along root surfaces and in the periodontal membrane in close proximity to the root surface of human primary and permanent teeth. Materials and methods. The material comprised extracted human teeth (11 primary teeth and six permanent teeth) from 10 different patients. Paraffin sections were prepared of each tooth and sections of each tooth were immunohistochemically stained with antibodies specific for membrane-bound RANKL and RANK. Results. The root surface and the periodontal membrane in close proximity to the root surface did not show immunoreactivity for RANKL. RANKL was only located in odontoblasts and in cells along denticles in one primary tooth. RANK was located in mononuclear cells in the pulp and in multinucleated odontoclasts along resorbed root surfaces and along resorbed dentin surfaces in the pulp in primary teeth and one permanent tooth. Conclusions. This study demonstrated RANK positivity in resorption areas in primary and permanent teeth. RANKL was positive in the pulp of one primary tooth. RANK expression in odontoclasts and RANKL expression in the pulp may indicate that RANK/RANKL play a role during resorption.",
author = "Bille, {Marie-Louise Bastholm} and Thomsen, {Axel Bjarke Korsgaard} and Andersen, {Thomas Levin} and Inger Kj{\ae}r",
year = "2012",
doi = "10.3109/00016357.2011.641585",
language = "English",
volume = "70",
pages = "265--271",
journal = "Acta Odontologica Scandinavica",
issn = "0001-6357",
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Immunolocalization of RANK and RANKL along the root surface and in the periodontal membrane of human primary and permanent teeth. / Bille, Marie-Louise Bastholm; Thomsen, Axel Bjarke Korsgaard; Andersen, Thomas Levin; Kjær, Inger.

I: Acta Odontologica Scandinavica, Bind 70, Nr. 4, 2012, s. 265-271.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Immunolocalization of RANK and RANKL along the root surface and in the periodontal membrane of human primary and permanent teeth

AU - Bille, Marie-Louise Bastholm

AU - Thomsen, Axel Bjarke Korsgaard

AU - Andersen, Thomas Levin

AU - Kjær, Inger

PY - 2012

Y1 - 2012

N2 - Abstract Objective. Root resorption, impaired tooth eruption and early tooth loss have been described in relation to diseases that involve defects in the RANK-RANKL-OPG-expression. The aim of the present immunhistochemical study was to localize and compare the reactions for RANK and membrane-bound RANKL along root surfaces and in the periodontal membrane in close proximity to the root surface of human primary and permanent teeth. Materials and methods. The material comprised extracted human teeth (11 primary teeth and six permanent teeth) from 10 different patients. Paraffin sections were prepared of each tooth and sections of each tooth were immunohistochemically stained with antibodies specific for membrane-bound RANKL and RANK. Results. The root surface and the periodontal membrane in close proximity to the root surface did not show immunoreactivity for RANKL. RANKL was only located in odontoblasts and in cells along denticles in one primary tooth. RANK was located in mononuclear cells in the pulp and in multinucleated odontoclasts along resorbed root surfaces and along resorbed dentin surfaces in the pulp in primary teeth and one permanent tooth. Conclusions. This study demonstrated RANK positivity in resorption areas in primary and permanent teeth. RANKL was positive in the pulp of one primary tooth. RANK expression in odontoclasts and RANKL expression in the pulp may indicate that RANK/RANKL play a role during resorption.

AB - Abstract Objective. Root resorption, impaired tooth eruption and early tooth loss have been described in relation to diseases that involve defects in the RANK-RANKL-OPG-expression. The aim of the present immunhistochemical study was to localize and compare the reactions for RANK and membrane-bound RANKL along root surfaces and in the periodontal membrane in close proximity to the root surface of human primary and permanent teeth. Materials and methods. The material comprised extracted human teeth (11 primary teeth and six permanent teeth) from 10 different patients. Paraffin sections were prepared of each tooth and sections of each tooth were immunohistochemically stained with antibodies specific for membrane-bound RANKL and RANK. Results. The root surface and the periodontal membrane in close proximity to the root surface did not show immunoreactivity for RANKL. RANKL was only located in odontoblasts and in cells along denticles in one primary tooth. RANK was located in mononuclear cells in the pulp and in multinucleated odontoclasts along resorbed root surfaces and along resorbed dentin surfaces in the pulp in primary teeth and one permanent tooth. Conclusions. This study demonstrated RANK positivity in resorption areas in primary and permanent teeth. RANKL was positive in the pulp of one primary tooth. RANK expression in odontoclasts and RANKL expression in the pulp may indicate that RANK/RANKL play a role during resorption.

U2 - 10.3109/00016357.2011.641585

DO - 10.3109/00016357.2011.641585

M3 - Journal article

C2 - 22397362

VL - 70

SP - 265

EP - 271

JO - Acta Odontologica Scandinavica

JF - Acta Odontologica Scandinavica

SN - 0001-6357

IS - 4

ER -