Immunohistochemical characterization of cloned lamb nephropathy

Andrew J Dawson, Tim J King, Ian Wilmut, Linda M Harkness, Brian G Kelly, Susan M Rhind

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Udgivelsesdato: 2004-Dec
OriginalsprogEngelsk
TidsskriftJournal of Histochemistry and Cytochemistry
Vol/bind52
Udgave nummer12
Sider (fra-til)1657-64
Antal sider7
ISSN0022-1554
DOI
StatusUdgivet - 1. dec. 2004
Udgivet eksterntJa

Fingeraftryk

Intermediate Filaments
Mesoderm
Kidney
Desmin
Vimentin
Smooth Muscle
Up-Regulation
Hydronephrosis
Urinary Tract
Actins
Epithelial Cells
Databases
Wounds and Injuries

Citer dette

Dawson, A. J., King, T. J., Wilmut, I., Harkness, L. M., Kelly, B. G., & Rhind, S. M. (2004). Immunohistochemical characterization of cloned lamb nephropathy. Journal of Histochemistry and Cytochemistry, 52(12), 1657-64. https://doi.org/10.1369/jhc.4A6367.2004
Dawson, Andrew J ; King, Tim J ; Wilmut, Ian ; Harkness, Linda M ; Kelly, Brian G ; Rhind, Susan M. / Immunohistochemical characterization of cloned lamb nephropathy. I: Journal of Histochemistry and Cytochemistry. 2004 ; Bind 52, Nr. 12. s. 1657-64.
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title = "Immunohistochemical characterization of cloned lamb nephropathy",
abstract = "Kidneys from lambs derived by nuclear transfer are frequently abnormal and are characterized by an enlarged pelvis and narrow medulla, consistent with lower urinary tract obstruction and development of variable hydronephrosis. The precise pathogenesis of this entity is unknown. Immunohistochemical staining for intermediate filaments was used to further characterize the lesions seen in this condition and was compared with age-matched control tissue. Major findings were upregulation of cytokeratin on damaged tubules, desmin and vimentin in undifferentiated mesenchyme, and smooth muscle actin in mesenchyme and on smooth muscle {"}collars{"} around dilated tubules. In addition, some cases showed reexpression of vimentin and desmin on proximal tubular epithelial cells. Taken together, these findings provide a valuable database for tracking the expression of intermediate filaments throughout renal development in sheep and have further characterized the nature of the response to injury by the developing kidney, a response that is characterized by proliferation of mesenchyme and both reexpression and upregulation of intermediate filaments within renal cells. In addition, the study has confirmed that the changes in cloned lamb nephropathy are established by day 85 of development.",
author = "Dawson, {Andrew J} and King, {Tim J} and Ian Wilmut and Harkness, {Linda M} and Kelly, {Brian G} and Rhind, {Susan M}",
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doi = "10.1369/jhc.4A6367.2004",
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Dawson, AJ, King, TJ, Wilmut, I, Harkness, LM, Kelly, BG & Rhind, SM 2004, 'Immunohistochemical characterization of cloned lamb nephropathy', Journal of Histochemistry and Cytochemistry, bind 52, nr. 12, s. 1657-64. https://doi.org/10.1369/jhc.4A6367.2004

Immunohistochemical characterization of cloned lamb nephropathy. / Dawson, Andrew J; King, Tim J; Wilmut, Ian; Harkness, Linda M; Kelly, Brian G; Rhind, Susan M.

I: Journal of Histochemistry and Cytochemistry, Bind 52, Nr. 12, 01.12.2004, s. 1657-64.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Immunohistochemical characterization of cloned lamb nephropathy

AU - Dawson, Andrew J

AU - King, Tim J

AU - Wilmut, Ian

AU - Harkness, Linda M

AU - Kelly, Brian G

AU - Rhind, Susan M

PY - 2004/12/1

Y1 - 2004/12/1

N2 - Kidneys from lambs derived by nuclear transfer are frequently abnormal and are characterized by an enlarged pelvis and narrow medulla, consistent with lower urinary tract obstruction and development of variable hydronephrosis. The precise pathogenesis of this entity is unknown. Immunohistochemical staining for intermediate filaments was used to further characterize the lesions seen in this condition and was compared with age-matched control tissue. Major findings were upregulation of cytokeratin on damaged tubules, desmin and vimentin in undifferentiated mesenchyme, and smooth muscle actin in mesenchyme and on smooth muscle "collars" around dilated tubules. In addition, some cases showed reexpression of vimentin and desmin on proximal tubular epithelial cells. Taken together, these findings provide a valuable database for tracking the expression of intermediate filaments throughout renal development in sheep and have further characterized the nature of the response to injury by the developing kidney, a response that is characterized by proliferation of mesenchyme and both reexpression and upregulation of intermediate filaments within renal cells. In addition, the study has confirmed that the changes in cloned lamb nephropathy are established by day 85 of development.

AB - Kidneys from lambs derived by nuclear transfer are frequently abnormal and are characterized by an enlarged pelvis and narrow medulla, consistent with lower urinary tract obstruction and development of variable hydronephrosis. The precise pathogenesis of this entity is unknown. Immunohistochemical staining for intermediate filaments was used to further characterize the lesions seen in this condition and was compared with age-matched control tissue. Major findings were upregulation of cytokeratin on damaged tubules, desmin and vimentin in undifferentiated mesenchyme, and smooth muscle actin in mesenchyme and on smooth muscle "collars" around dilated tubules. In addition, some cases showed reexpression of vimentin and desmin on proximal tubular epithelial cells. Taken together, these findings provide a valuable database for tracking the expression of intermediate filaments throughout renal development in sheep and have further characterized the nature of the response to injury by the developing kidney, a response that is characterized by proliferation of mesenchyme and both reexpression and upregulation of intermediate filaments within renal cells. In addition, the study has confirmed that the changes in cloned lamb nephropathy are established by day 85 of development.

U2 - 10.1369/jhc.4A6367.2004

DO - 10.1369/jhc.4A6367.2004

M3 - Journal article

VL - 52

SP - 1657

EP - 1664

JO - Journal of Histochemistry and Cytochemistry

JF - Journal of Histochemistry and Cytochemistry

SN - 0022-1554

IS - 12

ER -