@article{1b2d99600ca311dfaefb000ea68e967b,
title = "Immunofluorescence-based screening identifies germ cell associated microRNA 302 as an antagonist to p63 expression",
abstract = "The tumor suppressor homologue p63 is required for proper skin and limb development, but specific isoforms of it also act as a {"}guardian of the germline.{"} To gain insight into the regulation of p63 expression, we performed immunofluorescence-based screening assays. Using a large collection of microRNA expression plasmids, we identified microRNAs of the 302 cluster as potent suppressors of p63 accumulation in various cell species. MiR-302 reduces p63 protein and mRNA levels through two target sites within the p63 3' untranslated region. In testicular cancer cells, endogenous miR-302 contributes to the suppression of p63. MiR-302 might also contribute to the elimination of p63 in mature oocytes. Thus, miR-302 appears as part of a stringent regulatory mechanism for p63 in germ cells, reminiscent of the tight control for p53 levels in somatic cells.",
keywords = "3' Untranslated Regions, Base Sequence, Cell Line, Tumor, Fluorescent Antibody Technique, Gene Expression Regulation, Neoplastic, Germ Cells, Humans, MicroRNAs, Molecular Sequence Data, RNA, Messenger, Trans-Activators, Tumor Suppressor Proteins",
author = "Scheel, \{Andreas Hans Joachim\} and Ulrike Beyer and Reuven Agami and Matthias Dobbelstein",
year = "2009",
month = may,
day = "1",
language = "English",
volume = "8",
pages = "1426--32",
journal = "Cell Cycle",
issn = "1538-4101",
publisher = "Taylor \& Francis",
number = "9",
}