Immuno-regulatory T Cells may be Involved in preserving CD4 T cell counts in HIV-infected Long Term Non-Progressors and Controllers

Julie Christine Gaardbo, Andreas Ronit, Hans Jakob Hartling, Lise M R Gjerdrum, Karoline Springborg, Elisabeth Methner Ralfkiær, Kristina Thorsteinsson, Henrik Ullum, Ase B Andersen, Susanne D Nielsen

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Background: HIV-infected controllers control viral replication and maintain normal CD4 T cell counts. Long-term nonprogressors (LTNPs) also maintain normal CD4 T cell counts but have ongoing viral replication. We hypothesized that immunoregulatory mechanisms are involved in preserved CD4 T cell counts in controllers and in LTNPs. Methods: Twenty HIV-infected viremic controllers, 5 elite controllers (ECs), and 14 LTNPs were included in this cross-sectional study. For comparison, 25 progressors and 34 healthy controls were included. Regulatory T cells (Tregs), Treg subpopulations, CD161+Th17 cells, and CD3+CD8+CD161(high)Tc17 cells in peripheral blood were measured using flow cytometry. Tregs in lymphoid tissue were determined in tonsil biopsies and evaluated using immunolabeling. The production of transforming growth factor beta (TGF-beta), interleukin (IL)-10, and IL-17 upon stimulation with phytohemagglutinin in peripheral blood was determined by Luminex. Results: All groups of HIV-infected patients displayed similar percentages of Tregs in both peripheral blood and lymphoid tissue. However, a larger percentage of Tregs in ECs and LTNPs were activated compared with that in controls, progressors, and viremic controllers. Further, ECs as the only group of HIV-infected patients, displayed elevated percentages of CD161+Th17 cells, preserved CD3+CD8+CD161(high)Tc17 cells, and preserved IL-10 production. Conclusions: Overall, Treg percentage was similar in both blood and lymphoid tissue in all groups of patients and controls. However, both ECs and LTNPs displayed a large proportion of activated Tregs suggesting immunoregulatory mechanisms to be involved in preserving CD4 T cell counts in HIV-infected nonprogressors.
OriginalsprogEngelsk
TidsskriftJ A I D S
Vol/bind65
Udgave nummer1
Sider (fra-til)10-18
ISSN1525-4135
DOI
StatusUdgivet - 2014

Citer dette

Gaardbo, J. C., Ronit, A., Hartling, H. J., Gjerdrum, L. M. R., Springborg, K., Ralfkiær, E. M., ... Nielsen, S. D. (2014). Immuno-regulatory T Cells may be Involved in preserving CD4 T cell counts in HIV-infected Long Term Non-Progressors and Controllers. J A I D S, 65(1), 10-18. https://doi.org/10.1097/QAI.0b013e3182a7c932
Gaardbo, Julie Christine ; Ronit, Andreas ; Hartling, Hans Jakob ; Gjerdrum, Lise M R ; Springborg, Karoline ; Ralfkiær, Elisabeth Methner ; Thorsteinsson, Kristina ; Ullum, Henrik ; Andersen, Ase B ; Nielsen, Susanne D. / Immuno-regulatory T Cells may be Involved in preserving CD4 T cell counts in HIV-infected Long Term Non-Progressors and Controllers. I: J A I D S. 2014 ; Bind 65, Nr. 1. s. 10-18.
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title = "Immuno-regulatory T Cells may be Involved in preserving CD4 T cell counts in HIV-infected Long Term Non-Progressors and Controllers",
abstract = "Background: HIV-infected controllers control viral replication and maintain normal CD4 T cell counts. Long-term nonprogressors (LTNPs) also maintain normal CD4 T cell counts but have ongoing viral replication. We hypothesized that immunoregulatory mechanisms are involved in preserved CD4 T cell counts in controllers and in LTNPs. Methods: Twenty HIV-infected viremic controllers, 5 elite controllers (ECs), and 14 LTNPs were included in this cross-sectional study. For comparison, 25 progressors and 34 healthy controls were included. Regulatory T cells (Tregs), Treg subpopulations, CD161+Th17 cells, and CD3+CD8+CD161(high)Tc17 cells in peripheral blood were measured using flow cytometry. Tregs in lymphoid tissue were determined in tonsil biopsies and evaluated using immunolabeling. The production of transforming growth factor beta (TGF-beta), interleukin (IL)-10, and IL-17 upon stimulation with phytohemagglutinin in peripheral blood was determined by Luminex. Results: All groups of HIV-infected patients displayed similar percentages of Tregs in both peripheral blood and lymphoid tissue. However, a larger percentage of Tregs in ECs and LTNPs were activated compared with that in controls, progressors, and viremic controllers. Further, ECs as the only group of HIV-infected patients, displayed elevated percentages of CD161+Th17 cells, preserved CD3+CD8+CD161(high)Tc17 cells, and preserved IL-10 production. Conclusions: Overall, Treg percentage was similar in both blood and lymphoid tissue in all groups of patients and controls. However, both ECs and LTNPs displayed a large proportion of activated Tregs suggesting immunoregulatory mechanisms to be involved in preserving CD4 T cell counts in HIV-infected nonprogressors.",
author = "Gaardbo, {Julie Christine} and Andreas Ronit and Hartling, {Hans Jakob} and Gjerdrum, {Lise M R} and Karoline Springborg and Ralfki{\ae}r, {Elisabeth Methner} and Kristina Thorsteinsson and Henrik Ullum and Andersen, {Ase B} and Nielsen, {Susanne D}",
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Gaardbo, JC, Ronit, A, Hartling, HJ, Gjerdrum, LMR, Springborg, K, Ralfkiær, EM, Thorsteinsson, K, Ullum, H, Andersen, AB & Nielsen, SD 2014, 'Immuno-regulatory T Cells may be Involved in preserving CD4 T cell counts in HIV-infected Long Term Non-Progressors and Controllers', J A I D S, bind 65, nr. 1, s. 10-18. https://doi.org/10.1097/QAI.0b013e3182a7c932

Immuno-regulatory T Cells may be Involved in preserving CD4 T cell counts in HIV-infected Long Term Non-Progressors and Controllers. / Gaardbo, Julie Christine; Ronit, Andreas; Hartling, Hans Jakob; Gjerdrum, Lise M R; Springborg, Karoline; Ralfkiær, Elisabeth Methner; Thorsteinsson, Kristina; Ullum, Henrik; Andersen, Ase B; Nielsen, Susanne D.

I: J A I D S, Bind 65, Nr. 1, 2014, s. 10-18.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Immuno-regulatory T Cells may be Involved in preserving CD4 T cell counts in HIV-infected Long Term Non-Progressors and Controllers

AU - Gaardbo, Julie Christine

AU - Ronit, Andreas

AU - Hartling, Hans Jakob

AU - Gjerdrum, Lise M R

AU - Springborg, Karoline

AU - Ralfkiær, Elisabeth Methner

AU - Thorsteinsson, Kristina

AU - Ullum, Henrik

AU - Andersen, Ase B

AU - Nielsen, Susanne D

PY - 2014

Y1 - 2014

N2 - Background: HIV-infected controllers control viral replication and maintain normal CD4 T cell counts. Long-term nonprogressors (LTNPs) also maintain normal CD4 T cell counts but have ongoing viral replication. We hypothesized that immunoregulatory mechanisms are involved in preserved CD4 T cell counts in controllers and in LTNPs. Methods: Twenty HIV-infected viremic controllers, 5 elite controllers (ECs), and 14 LTNPs were included in this cross-sectional study. For comparison, 25 progressors and 34 healthy controls were included. Regulatory T cells (Tregs), Treg subpopulations, CD161+Th17 cells, and CD3+CD8+CD161(high)Tc17 cells in peripheral blood were measured using flow cytometry. Tregs in lymphoid tissue were determined in tonsil biopsies and evaluated using immunolabeling. The production of transforming growth factor beta (TGF-beta), interleukin (IL)-10, and IL-17 upon stimulation with phytohemagglutinin in peripheral blood was determined by Luminex. Results: All groups of HIV-infected patients displayed similar percentages of Tregs in both peripheral blood and lymphoid tissue. However, a larger percentage of Tregs in ECs and LTNPs were activated compared with that in controls, progressors, and viremic controllers. Further, ECs as the only group of HIV-infected patients, displayed elevated percentages of CD161+Th17 cells, preserved CD3+CD8+CD161(high)Tc17 cells, and preserved IL-10 production. Conclusions: Overall, Treg percentage was similar in both blood and lymphoid tissue in all groups of patients and controls. However, both ECs and LTNPs displayed a large proportion of activated Tregs suggesting immunoregulatory mechanisms to be involved in preserving CD4 T cell counts in HIV-infected nonprogressors.

AB - Background: HIV-infected controllers control viral replication and maintain normal CD4 T cell counts. Long-term nonprogressors (LTNPs) also maintain normal CD4 T cell counts but have ongoing viral replication. We hypothesized that immunoregulatory mechanisms are involved in preserved CD4 T cell counts in controllers and in LTNPs. Methods: Twenty HIV-infected viremic controllers, 5 elite controllers (ECs), and 14 LTNPs were included in this cross-sectional study. For comparison, 25 progressors and 34 healthy controls were included. Regulatory T cells (Tregs), Treg subpopulations, CD161+Th17 cells, and CD3+CD8+CD161(high)Tc17 cells in peripheral blood were measured using flow cytometry. Tregs in lymphoid tissue were determined in tonsil biopsies and evaluated using immunolabeling. The production of transforming growth factor beta (TGF-beta), interleukin (IL)-10, and IL-17 upon stimulation with phytohemagglutinin in peripheral blood was determined by Luminex. Results: All groups of HIV-infected patients displayed similar percentages of Tregs in both peripheral blood and lymphoid tissue. However, a larger percentage of Tregs in ECs and LTNPs were activated compared with that in controls, progressors, and viremic controllers. Further, ECs as the only group of HIV-infected patients, displayed elevated percentages of CD161+Th17 cells, preserved CD3+CD8+CD161(high)Tc17 cells, and preserved IL-10 production. Conclusions: Overall, Treg percentage was similar in both blood and lymphoid tissue in all groups of patients and controls. However, both ECs and LTNPs displayed a large proportion of activated Tregs suggesting immunoregulatory mechanisms to be involved in preserving CD4 T cell counts in HIV-infected nonprogressors.

U2 - 10.1097/QAI.0b013e3182a7c932

DO - 10.1097/QAI.0b013e3182a7c932

M3 - Journal article

VL - 65

SP - 10

EP - 18

JO - J A I D S

JF - J A I D S

SN - 1525-4135

IS - 1

ER -