Immobilization of lipase B within micron-sized poly-N-isopropylacrylamide hydrogel particles by solvent exchange

Kornelia Gawlitza, Changzhu Wu, Radostina Georgieva, Dayang Wang, Marion B. Ansorge-Schumacher, Regine Von Klitzing*

*Kontaktforfatter

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Abstract

The aim of the present work is the use of a water soluble enzyme in an organic solvent, still with a pronounced catalytic activity. Therefore, lipase B from Candida antarctica (CalB) is immobilized within micron-sized thermosensitive p-NIPAM hydrogel particles using a solvent exchange from polar to organic solvents. The absorbed amount of CalB is investigated at different immobilization temperatures. Confocal laser scanning microscopy (CLSM) shows that CalB is homogeneously distributed within the polymer network. An enhanced specific activity of CalB in n-hexane is achieved after immobilization within the p-NIPAM microgels. In order to get information on the supply of the substrate depending on the temperature, the activity is determined at different reaction temperatures. Additionally, the system is stable in the organic solvent, namely n-hexane, and shows a good reusability. This journal is

OriginalsprogEngelsk
TidsskriftPhysical Chemistry Chemical Physics
Vol/bind14
Udgave nummer27
Sider (fra-til)9594-9600
ISSN1463-9076
DOI
StatusUdgivet - 21. jul. 2012
Udgivet eksterntJa

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