IFCC methods for the measurement of catalytic concentration of enzymes. I. General considerations concerning the determination of the catalytic concentration of an enzyme in the blood serum or plasma of man

G. N. Bowers, H. U. Bergmeyer, M. Horder, D. W. Moss

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The central consideration in these efforts to define an IFCC Method should be to achieve a blend of maximum reliability and optimum conditions. Any mechanism which establishes an IFCC Method in clinical enzymology should also include a means for change so that further research is not inhibited. The diagnostic value of determining the catalytic activity of an enzyme in blood serum or plasma which has made it important in medicine should be maintained or enhanced. Each method should contribute to differentiating healthy persons from individuals with diseases known to be associated with an elevation (or depression) of the catalytic activity. The effect on total activity of alterations in the proportions of multiple molecular forms of a particular enzyme found in human serum must also be considered. The major aim of an IFCC Method is to achieve maximum reproducibility of analysis both within each laboratory and between laboratories thorughout the world. Therefore, the sensitivity, precision, specificity and accuracy of the analytical measuring system must be of primary importance.

TidsskriftJournal of Clinical Chemistry and Clinical Biochemistry
Udgave nummer1
Sider (fra-til)89-95
Antal sider7
StatusUdgivet - 1. jan. 1980