Identifying sources and estimating glandular output of salivary TIMP-1

L Holten-Andersen, S Beier Jensen, A Bardow, J Harslund, M Thaysen-Andersen, U Lademann, P Autzen Usher, H Offenberg, P Højrup, J Reibel, H J Nielsen, N Brunner, B Nauntofte

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Objective. Tissue inhibitor of metalloproteinases 1 (TIMP-1) has been identified as a potential biomarker in diseases such as cancer, cardiovascular diseases and diabetes. Since TIMP-1 resides in most tissues and bodily fluids, we evaluated the potential of using saliva to obtain reproducible TIMP-1 measurements in a non-invasive manner. Material and methods. Samples of unstimulated and stimulated whole saliva and saliva collected from individual glands were analysed for TIMP-1 content. A TIMP-1 ELISA was validated for use in saliva testing and the most optimal sampling and handling procedures for reproducible measurements identified. Western blotting and MALDI-TOF mass spectrometry were used for confirmatory analyses. Results. The TIMP-1 ELISA was found suitable for saliva measurements. All saliva secretions contained TIMP-1, but in different concentrations ranging from 2.81 ng/mL in submandibular/sublingual saliva to 173.88 ng/mL in parotid saliva. TIMP-1 concentrations were influenced to a varying degree by fluctuations in flow. We found the lowest output in submandibular/sublingual saliva stimulated with 0.5 % citric acid (3.56 ng/min) and highest output in chewing-stimulated whole saliva (267.01 ng/min). Conclusion. This study shows that saliva contains authentic TIMP-1, the concentration of which was found to depend on gland type and salivary flow. Stimulated whole saliva is suggested as a reliable and easily accessible source for TIMP-1 determinations in bodily fluids.
OriginalsprogEngelsk
BogserieScandinavian Journal of Clinical and Laboratory Investigation. Supplement
Vol/bind68
Udgave nummer7
Sider (fra-til)548-554
Antal sider6
ISSN0085-591X
DOI
StatusUdgivet - 2008

Fingeraftryk

Tissue Inhibitor of Metalloproteinase-1
Saliva
Mastication
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry

Citer dette

Holten-Andersen, L., Beier Jensen, S., Bardow, A., Harslund, J., Thaysen-Andersen, M., Lademann, U., ... Nauntofte, B. (2008). Identifying sources and estimating glandular output of salivary TIMP-1. Scandinavian Journal of Clinical and Laboratory Investigation. Supplement, 68(7), 548-554. https://doi.org/10.1080/00365510701883180
Holten-Andersen, L ; Beier Jensen, S ; Bardow, A ; Harslund, J ; Thaysen-Andersen, M ; Lademann, U ; Autzen Usher, P ; Offenberg, H ; Højrup, P ; Reibel, J ; Nielsen, H J ; Brunner, N ; Nauntofte, B. / Identifying sources and estimating glandular output of salivary TIMP-1. I: Scandinavian Journal of Clinical and Laboratory Investigation. Supplement. 2008 ; Bind 68, Nr. 7. s. 548-554.
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title = "Identifying sources and estimating glandular output of salivary TIMP-1",
abstract = "Objective. Tissue inhibitor of metalloproteinases 1 (TIMP-1) has been identified as a potential biomarker in diseases such as cancer, cardiovascular diseases and diabetes. Since TIMP-1 resides in most tissues and bodily fluids, we evaluated the potential of using saliva to obtain reproducible TIMP-1 measurements in a non-invasive manner. Material and methods. Samples of unstimulated and stimulated whole saliva and saliva collected from individual glands were analysed for TIMP-1 content. A TIMP-1 ELISA was validated for use in saliva testing and the most optimal sampling and handling procedures for reproducible measurements identified. Western blotting and MALDI-TOF mass spectrometry were used for confirmatory analyses. Results. The TIMP-1 ELISA was found suitable for saliva measurements. All saliva secretions contained TIMP-1, but in different concentrations ranging from 2.81 ng/mL in submandibular/sublingual saliva to 173.88 ng/mL in parotid saliva. TIMP-1 concentrations were influenced to a varying degree by fluctuations in flow. We found the lowest output in submandibular/sublingual saliva stimulated with 0.5 {\%} citric acid (3.56 ng/min) and highest output in chewing-stimulated whole saliva (267.01 ng/min). Conclusion. This study shows that saliva contains authentic TIMP-1, the concentration of which was found to depend on gland type and salivary flow. Stimulated whole saliva is suggested as a reliable and easily accessible source for TIMP-1 determinations in bodily fluids.",
author = "L Holten-Andersen and {Beier Jensen}, S and A Bardow and J Harslund and M Thaysen-Andersen and U Lademann and {Autzen Usher}, P and H Offenberg and P H{\o}jrup and J Reibel and Nielsen, {H J} and N Brunner and B Nauntofte",
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volume = "68",
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Holten-Andersen, L, Beier Jensen, S, Bardow, A, Harslund, J, Thaysen-Andersen, M, Lademann, U, Autzen Usher, P, Offenberg, H, Højrup, P, Reibel, J, Nielsen, HJ, Brunner, N & Nauntofte, B 2008, 'Identifying sources and estimating glandular output of salivary TIMP-1', Scandinavian Journal of Clinical and Laboratory Investigation. Supplement, bind 68, nr. 7, s. 548-554. https://doi.org/10.1080/00365510701883180

Identifying sources and estimating glandular output of salivary TIMP-1. / Holten-Andersen, L; Beier Jensen, S; Bardow, A; Harslund, J; Thaysen-Andersen, M; Lademann, U; Autzen Usher, P; Offenberg, H; Højrup, P; Reibel, J; Nielsen, H J; Brunner, N; Nauntofte, B.

I: Scandinavian Journal of Clinical and Laboratory Investigation. Supplement, Bind 68, Nr. 7, 2008, s. 548-554.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Identifying sources and estimating glandular output of salivary TIMP-1

AU - Holten-Andersen, L

AU - Beier Jensen, S

AU - Bardow, A

AU - Harslund, J

AU - Thaysen-Andersen, M

AU - Lademann, U

AU - Autzen Usher, P

AU - Offenberg, H

AU - Højrup, P

AU - Reibel, J

AU - Nielsen, H J

AU - Brunner, N

AU - Nauntofte, B

PY - 2008

Y1 - 2008

N2 - Objective. Tissue inhibitor of metalloproteinases 1 (TIMP-1) has been identified as a potential biomarker in diseases such as cancer, cardiovascular diseases and diabetes. Since TIMP-1 resides in most tissues and bodily fluids, we evaluated the potential of using saliva to obtain reproducible TIMP-1 measurements in a non-invasive manner. Material and methods. Samples of unstimulated and stimulated whole saliva and saliva collected from individual glands were analysed for TIMP-1 content. A TIMP-1 ELISA was validated for use in saliva testing and the most optimal sampling and handling procedures for reproducible measurements identified. Western blotting and MALDI-TOF mass spectrometry were used for confirmatory analyses. Results. The TIMP-1 ELISA was found suitable for saliva measurements. All saliva secretions contained TIMP-1, but in different concentrations ranging from 2.81 ng/mL in submandibular/sublingual saliva to 173.88 ng/mL in parotid saliva. TIMP-1 concentrations were influenced to a varying degree by fluctuations in flow. We found the lowest output in submandibular/sublingual saliva stimulated with 0.5 % citric acid (3.56 ng/min) and highest output in chewing-stimulated whole saliva (267.01 ng/min). Conclusion. This study shows that saliva contains authentic TIMP-1, the concentration of which was found to depend on gland type and salivary flow. Stimulated whole saliva is suggested as a reliable and easily accessible source for TIMP-1 determinations in bodily fluids.

AB - Objective. Tissue inhibitor of metalloproteinases 1 (TIMP-1) has been identified as a potential biomarker in diseases such as cancer, cardiovascular diseases and diabetes. Since TIMP-1 resides in most tissues and bodily fluids, we evaluated the potential of using saliva to obtain reproducible TIMP-1 measurements in a non-invasive manner. Material and methods. Samples of unstimulated and stimulated whole saliva and saliva collected from individual glands were analysed for TIMP-1 content. A TIMP-1 ELISA was validated for use in saliva testing and the most optimal sampling and handling procedures for reproducible measurements identified. Western blotting and MALDI-TOF mass spectrometry were used for confirmatory analyses. Results. The TIMP-1 ELISA was found suitable for saliva measurements. All saliva secretions contained TIMP-1, but in different concentrations ranging from 2.81 ng/mL in submandibular/sublingual saliva to 173.88 ng/mL in parotid saliva. TIMP-1 concentrations were influenced to a varying degree by fluctuations in flow. We found the lowest output in submandibular/sublingual saliva stimulated with 0.5 % citric acid (3.56 ng/min) and highest output in chewing-stimulated whole saliva (267.01 ng/min). Conclusion. This study shows that saliva contains authentic TIMP-1, the concentration of which was found to depend on gland type and salivary flow. Stimulated whole saliva is suggested as a reliable and easily accessible source for TIMP-1 determinations in bodily fluids.

U2 - 10.1080/00365510701883180

DO - 10.1080/00365510701883180

M3 - Journal article

VL - 68

SP - 548

EP - 554

JO - Scandinavian Journal of Clinical and Laboratory Investigation. Supplement

JF - Scandinavian Journal of Clinical and Laboratory Investigation. Supplement

SN - 0085-591X

IS - 7

ER -