Identification of the subunit of cAMP receptor protein (CRP) that functionally interacts with CytR in CRP-CytR-mediated transcriptional repression.

K L Meibom, B H Kallipolitis, R H Ebright, P Valentin-Hansen

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Udgivelsesdato: 2000-Apr-21
OriginalsprogEngelsk
TidsskriftJournal of Biological Chemistry
Vol/bind275
Udgave nummer16
Sider (fra-til)11951-6
Antal sider5
ISSN0021-9258
DOI
StatusUdgivet - 21. apr. 2000

Fingeraftryk

Cyclic AMP Receptor Protein
Regulon
Protein Subunits
Dimers
Escherichia coli

Citer dette

@article{9c868520323511dda26c000ea68e967b,
title = "Identification of the subunit of cAMP receptor protein (CRP) that functionally interacts with CytR in CRP-CytR-mediated transcriptional repression.",
abstract = "At promoters of the Escherichia coli CytR regulon, the cAMP receptor protein (CRP) interacts with the repressor CytR to form transcriptionally inactive CRP-CytR-promoter or (CRP)(2)-CytR-promoter complexes. Here, using {"}oriented heterodimer{"} analysis, we show that only one subunit of the CRP dimer, the subunit proximal to CytR, functionally interacts with CytR in CRP-CytR-promoter and (CRP)(2)-CytR-promoter complexes. Our results provide information about the architecture of CRP-CytR-promoter and (CRP)(2)-CytR-promoter complexes and rule out the proposal that masking of activating region 2 of CRP is responsible for the transcriptional inactivity of the complexes.",
keywords = "Base Sequence, Cyclic AMP Receptor Protein, Escherichia coli, Escherichia coli Proteins, Models, Molecular, Molecular Sequence Data, Promoter Regions (Genetics), Protein Conformation, Repressor Proteins, Transcription, Genetic",
author = "Meibom, {K L} and Kallipolitis, {B H} and Ebright, {R H} and P Valentin-Hansen",
year = "2000",
month = "4",
day = "21",
doi = "10.1074/jbc.275.16.11951",
language = "English",
volume = "275",
pages = "11951--6",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology, Inc.",
number = "16",

}

Identification of the subunit of cAMP receptor protein (CRP) that functionally interacts with CytR in CRP-CytR-mediated transcriptional repression. / Meibom, K L; Kallipolitis, B H; Ebright, R H; Valentin-Hansen, P.

I: Journal of Biological Chemistry, Bind 275, Nr. 16, 21.04.2000, s. 11951-6.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Identification of the subunit of cAMP receptor protein (CRP) that functionally interacts with CytR in CRP-CytR-mediated transcriptional repression.

AU - Meibom, K L

AU - Kallipolitis, B H

AU - Ebright, R H

AU - Valentin-Hansen, P

PY - 2000/4/21

Y1 - 2000/4/21

N2 - At promoters of the Escherichia coli CytR regulon, the cAMP receptor protein (CRP) interacts with the repressor CytR to form transcriptionally inactive CRP-CytR-promoter or (CRP)(2)-CytR-promoter complexes. Here, using "oriented heterodimer" analysis, we show that only one subunit of the CRP dimer, the subunit proximal to CytR, functionally interacts with CytR in CRP-CytR-promoter and (CRP)(2)-CytR-promoter complexes. Our results provide information about the architecture of CRP-CytR-promoter and (CRP)(2)-CytR-promoter complexes and rule out the proposal that masking of activating region 2 of CRP is responsible for the transcriptional inactivity of the complexes.

AB - At promoters of the Escherichia coli CytR regulon, the cAMP receptor protein (CRP) interacts with the repressor CytR to form transcriptionally inactive CRP-CytR-promoter or (CRP)(2)-CytR-promoter complexes. Here, using "oriented heterodimer" analysis, we show that only one subunit of the CRP dimer, the subunit proximal to CytR, functionally interacts with CytR in CRP-CytR-promoter and (CRP)(2)-CytR-promoter complexes. Our results provide information about the architecture of CRP-CytR-promoter and (CRP)(2)-CytR-promoter complexes and rule out the proposal that masking of activating region 2 of CRP is responsible for the transcriptional inactivity of the complexes.

KW - Base Sequence

KW - Cyclic AMP Receptor Protein

KW - Escherichia coli

KW - Escherichia coli Proteins

KW - Models, Molecular

KW - Molecular Sequence Data

KW - Promoter Regions (Genetics)

KW - Protein Conformation

KW - Repressor Proteins

KW - Transcription, Genetic

U2 - 10.1074/jbc.275.16.11951

DO - 10.1074/jbc.275.16.11951

M3 - Journal article

C2 - 10766824

VL - 275

SP - 11951

EP - 11956

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 16

ER -