Identification of Salt-Tolerant Sinorhizobium sp Strain BL3 Membrane Proteins Based on Proteomics

Waraporn Tanthanuch, Shabaz Mohammed, Rune Matthiesen, Montarop Yamabhai, Monchai Manassila, Ole Nørregaard Jensen, Nantakorn Boonkerd, Neung Teaumroong

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Sinorhizobium sp. BL3 is a salt-tolerant strain that can fix atmospheric nitrogen in symbiosis with leguminous host plants under salt-stress conditions. Since cell membranes are the first barrier to environmental change, it is interesting to explore the membrane proteins within this protective barrier under salt stress. The protein contents of membrane-enriched fractions obtained from BL3 were analyzed by nanoflow liquid chromatography interfaced with electrospray ionization tandem mass spectrometry. A total of 105 membrane proteins were identified. These proteins could be classified into 17 functional categories, the two biggest of which were energy production and conversion, and proteins not in clusters of orthologous groups (COGs). In addition, a comparative analysis of membrane proteins between salt-stressed and non-stressed BL3 cells was conducted using a membrane enrichment method and off-line SCX fractionation coupled to nanoLC-MS/MS. These techniques would be useful for further comparative analysis of membrane proteins that function in the response to environmental stress.
OriginalsprogEngelsk
TidsskriftMicrobes and Environments
Vol/bind25
Udgave nummer4
Sider (fra-til)275-280
ISSN1342-6311
DOI
StatusUdgivet - 1. jan. 2010

Fingeraftryk

Sinorhizobium
proteomics
membrane proteins
membrane
salt
salts
protein
salt stress
liquid chromatography
symbiosis
cell membranes
fractionation
host plants
proteins
protein content
environmental stress
energy
nitrogen
host plant
methodology

Citer dette

Tanthanuch, W., Mohammed, S., Matthiesen, R., Yamabhai, M., Manassila, M., Jensen, O. N., ... Teaumroong, N. (2010). Identification of Salt-Tolerant Sinorhizobium sp Strain BL3 Membrane Proteins Based on Proteomics. Microbes and Environments, 25(4), 275-280. https://doi.org/10.1264/jsme2.ME09185
Tanthanuch, Waraporn ; Mohammed, Shabaz ; Matthiesen, Rune ; Yamabhai, Montarop ; Manassila, Monchai ; Jensen, Ole Nørregaard ; Boonkerd, Nantakorn ; Teaumroong, Neung . / Identification of Salt-Tolerant Sinorhizobium sp Strain BL3 Membrane Proteins Based on Proteomics. I: Microbes and Environments. 2010 ; Bind 25, Nr. 4. s. 275-280.
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abstract = "Sinorhizobium sp. BL3 is a salt-tolerant strain that can fix atmospheric nitrogen in symbiosis with leguminous host plants under salt-stress conditions. Since cell membranes are the first barrier to environmental change, it is interesting to explore the membrane proteins within this protective barrier under salt stress. The protein contents of membrane-enriched fractions obtained from BL3 were analyzed by nanoflow liquid chromatography interfaced with electrospray ionization tandem mass spectrometry. A total of 105 membrane proteins were identified. These proteins could be classified into 17 functional categories, the two biggest of which were energy production and conversion, and proteins not in clusters of orthologous groups (COGs). In addition, a comparative analysis of membrane proteins between salt-stressed and non-stressed BL3 cells was conducted using a membrane enrichment method and off-line SCX fractionation coupled to nanoLC-MS/MS. These techniques would be useful for further comparative analysis of membrane proteins that function in the response to environmental stress.",
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Tanthanuch, W, Mohammed, S, Matthiesen, R, Yamabhai, M, Manassila, M, Jensen, ON, Boonkerd, N & Teaumroong, N 2010, 'Identification of Salt-Tolerant Sinorhizobium sp Strain BL3 Membrane Proteins Based on Proteomics', Microbes and Environments, bind 25, nr. 4, s. 275-280. https://doi.org/10.1264/jsme2.ME09185

Identification of Salt-Tolerant Sinorhizobium sp Strain BL3 Membrane Proteins Based on Proteomics. / Tanthanuch, Waraporn; Mohammed, Shabaz; Matthiesen, Rune; Yamabhai, Montarop; Manassila, Monchai; Jensen, Ole Nørregaard; Boonkerd, Nantakorn; Teaumroong, Neung .

I: Microbes and Environments, Bind 25, Nr. 4, 01.01.2010, s. 275-280.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Identification of Salt-Tolerant Sinorhizobium sp Strain BL3 Membrane Proteins Based on Proteomics

AU - Tanthanuch, Waraporn

AU - Mohammed, Shabaz

AU - Matthiesen, Rune

AU - Yamabhai, Montarop

AU - Manassila, Monchai

AU - Jensen, Ole Nørregaard

AU - Boonkerd, Nantakorn

AU - Teaumroong, Neung

PY - 2010/1/1

Y1 - 2010/1/1

N2 - Sinorhizobium sp. BL3 is a salt-tolerant strain that can fix atmospheric nitrogen in symbiosis with leguminous host plants under salt-stress conditions. Since cell membranes are the first barrier to environmental change, it is interesting to explore the membrane proteins within this protective barrier under salt stress. The protein contents of membrane-enriched fractions obtained from BL3 were analyzed by nanoflow liquid chromatography interfaced with electrospray ionization tandem mass spectrometry. A total of 105 membrane proteins were identified. These proteins could be classified into 17 functional categories, the two biggest of which were energy production and conversion, and proteins not in clusters of orthologous groups (COGs). In addition, a comparative analysis of membrane proteins between salt-stressed and non-stressed BL3 cells was conducted using a membrane enrichment method and off-line SCX fractionation coupled to nanoLC-MS/MS. These techniques would be useful for further comparative analysis of membrane proteins that function in the response to environmental stress.

AB - Sinorhizobium sp. BL3 is a salt-tolerant strain that can fix atmospheric nitrogen in symbiosis with leguminous host plants under salt-stress conditions. Since cell membranes are the first barrier to environmental change, it is interesting to explore the membrane proteins within this protective barrier under salt stress. The protein contents of membrane-enriched fractions obtained from BL3 were analyzed by nanoflow liquid chromatography interfaced with electrospray ionization tandem mass spectrometry. A total of 105 membrane proteins were identified. These proteins could be classified into 17 functional categories, the two biggest of which were energy production and conversion, and proteins not in clusters of orthologous groups (COGs). In addition, a comparative analysis of membrane proteins between salt-stressed and non-stressed BL3 cells was conducted using a membrane enrichment method and off-line SCX fractionation coupled to nanoLC-MS/MS. These techniques would be useful for further comparative analysis of membrane proteins that function in the response to environmental stress.

U2 - 10.1264/jsme2.ME09185

DO - 10.1264/jsme2.ME09185

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SP - 275

EP - 280

JO - Microbes and Environments

JF - Microbes and Environments

SN - 1342-6311

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ER -