@article{ca5e9e60d94d11dc860c000ea68e967b,
title = "Identification of human T cell targets recognized during Chlamydia trachomatis genital infection",
abstract = "The specificity of the human T cell response to Chlamydia trachomatis was investigated by stimulating lymphocytes from 16 case patients with urogenital infection by use of a size-fractionated serovar D lysate. Considerable heterogeneity was found among case patients, and multiple protein fractions were recognized in each specimen. Mass spectrometry analysis of the 30-42-kDa T cell-stimulating region identified 10 C. trachomatis proteins. Of these, CT583, CT603, and CT610 were identified as strong antigens that induced significantly higher levels of IFN- gamma secretion in PBMCs from case patients, compared with PBMCs from control donors. All 3 proteins were recognized in specimens from case patients infected with serovars D-F, the most prevalent serovars. McDonald-Kreitman and Tajima's D tests involving clinical isolates from the same samples showed evidence for frequency-dependent selection on ct583. We predict that CT583 is a target of acquired protective immune responses in humans.",
keywords = "Case-Control Studies, Chlamydia Infections, Chlamydia trachomatis, DNA Primers, DNA, Bacterial, Humans, Polymerase Chain Reaction, T-Lymphocytes",
author = "Olsen, {Anja Weinreich} and Frank Follmann and Peter H{\o}jrup and Robert Leah and Carsten Sand and Peter Andersen and Michael Theisen",
year = "2007",
month = nov,
day = "15",
doi = "10.1086/522524",
language = "English",
volume = "196",
pages = "1546--1552",
journal = "The Journal of Infectious Diseases",
issn = "0022-1899",
publisher = "Oxford University Press",
number = "10",
}